Replication of neurovirulent equine herpesvirus type 1 (EHV-1) in CD172a(+) monocytic cells.
Abstract
Equine herpesvirus type 1 (EHV-1) is responsible for respiratory disorders, abortion and myeloencephalopathy (EHM) in horses. Two pathotypes of EHV-1 strains are circulating in the field: neurovirulent (N) and non-neurovirulent (NN). For both strains, CD172a+ monocytic cells are one of the main carrier cells of EHV-1 during primary infection, allowing the virus to invade the horse's body. Recently, we showed that EHV-1 NN strains showed a restricted and delayed replication in CD172a+ cells. Here we characterize the in vitro replication kinetics of two EHV-1N strains in CD172a+ cells and investigate if the replication of these strains is similarly silenced as shown for EHV-1 NN strains. We found that EHV-1N replication was restricted to 7-8% in CD172a+ cells compared to 100% in control RK-13 cells. EHV-1N replication was not delayed in CD172a+ cells but virus production was significant lower (10 3.0 TCID 50/10 5 inoculated cells) than in RK-13 cells (10 8.5 TCID 50/10 5 inoculated cells). Approximately 0.04% of CD172a+ cells produced and transmitted infectious EHV-1 to neighbour cells compared to 65% of RK-13 cells. Unlike what we observed for the NN strain, pretreatment of CD172a+ cells with histone deacetylases inhibitors (HDACi) did not influence the replication of EHV-1N strains in these cells. Overall, these results show that the EHV-1 replication of N strains in CD172a+ cells differs from that observed for NN strains, which may contribute to their different pathogeneses in vivo.
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Self-citationEntry of equid herpesvirus 1 into CD172a+ monocytic cells.
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The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1
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BackgroundSutton, G., Thieulent, C., Fortier, C. I., Hue, E. S., Marcillaud‐Pitel, C., Pléau, A., Deslis, A., Guitton, E., Paillot, R., & Pronost, S. L. (2020) · Viruses · doi:10.3390/v12101160 · Cited by 38 · see citing works
“In accordance with other studies, the authors suggested that N752 had adapted its immune evasion strategy after co-evolving from D752 strains with the host immune system [52,54,55].”
ResultEquine Herpesvirus 1 Bridles T Lymphocytes To Reach Its Target Organs
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“cells following infection with neurovirulent EHV1 variants (15).”
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Equine Herpesvirus Infections: Treatment Progress and Challenges in Horses and Donkeys
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“Viral replication remains highly restricted in primary carrier cells, particularly CD172a+ monocytic cells, with non-neurovirulent strains specifically delaying replication through epigenetic mechanisms involving silencing of viral gene expression by histone deacetylases (HDACs) [195,196].”
Equine histones are mobilized within equid alphaherpesvirus 1 (EHV1) replication compartments
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Mucosal T cell activation pathways are upregulated by equine herpesvirus type 1 infection
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“As the infection progresses, immune cells in the URT may become infected, facilitating viral trafficking to the lymph nodes, and establishing a cell-associated viremia [20–24].”
- Authors
- Laval, K., Van Cleemput, J., Poelaert, K. C., Brown, I. K., & Nauwynck, H. J.
- Affiliations
- Ghent University (BE)
- Corresponding author
- Nauwynck, Hans J.
- Venue
- Comparative Immunology Microbiology and Infectious Diseases
- Publisher
- Elsevier BV
- Published
- 2017-02
- Volume
- 50
- Pages
- 58-62
- ISSN
- 0147-9571 (ISSN-L)
- OpenAlex
- W2555142972
- Cited by
- 14
- References
- 14
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 3 · 2017: 1
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Comparative Immunology Microbiology and Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Funders
- Belgian Science Policy (BELSPO)
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Keywords
- equine herpesvirus 1, viral replication kinetics, myeloencephalopathy, histone deacetylase inhibitors, cell-to-cell transmission, RK13 cells, horses, viral pathogenesis
- MeSH terms
- Animals, Cells, Cultured, Herpesvirus 1, Equid, Horses, Monocytes, Virus Replication
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