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Cited by 43Hormonal and reproductive studiesClinical Laboratory Practices and Quality ControlAnalytical Methods in Pharmaceuticals

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Direct measurement of serum free testosterone by ultrafiltration followed by liquid chromatography tandem mass spectrometry

Chen, Yu, Yazdanpanah, Mehrdad, Wang, Xiao Yan, Hoffman, Barry R., Diamandis, Eleftherios P., Wong, Pui-Yuen·2010·Clinical Biochemistry
DOI 10.1016/j.clinbiochem.2009.12.005
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Abstract

Background Currently there is no reliable method suitable for routine measurement of serum free testosterone (FT). Aim To develop such a method involving liquid chromatography tandem mass spectrometry (LC-IDMS/MS) that directly detects and quantifies the FT present in serum. Methods Ultrafiltrate testosterone obtained from 0.5 mL of serum was partially purified by liquid/liquid extraction and quantified using an Agilent 1200 Series HPLC system coupled to an API 5000 mass spectrometer equipped with an atmospheric pressure chemical ionization ion source. Using split samples serum free testosterone was compared between direct ultrafiltration (UF) coupled LC-MS/MS, analogue FT immunoassay, free testosterone calculated from mass action equations (cFT) and with equilibrium dialysis (ED) coupled LC-MS/MS. Results Total imprecision determined over twenty runs was Conclusion We present a relatively simple UF coupled LC-MS/MS definitive method that measures serum free testosterone. The method is relatively fast, reliable and is suitable for the routine clinical laboratory practice.

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Crossref · 10.1016/j.clinbiochem.2009.12.005

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Metadata

Authors
Chen, Y., Yazdanpanah, M., Wang, X. Y., Hoffman, B. R., Diamandis, E. P., & Wong, P.-Y.
Affiliations
  • University of Toronto (CA)
  • University Health Network (CA)
  • Mount Sinai Hospital (CA)
Corresponding author
Wong, Pui-Yuen
ORCID
Venue
Clinical Biochemistry
Publisher
Elsevier BV
Published
2010-03
Volume
43
Issue
4-5
Pages
490-496
ISSN
0009-9120 (ISSN-L)
DOI
10.1016/j.clinbiochem.2009.12.005
OpenAlex
W2035349952
Cited by
43
References
2
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 6 · 2012: 8
FWCI
3.15
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Clinical Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
Keywords
serum free testosterone, ultrafiltration, liquid chromatography-tandem mass spectrometry, equilibrium dialysis, immunoassay comparison, mass action equations, total testosterone
MeSH terms
Adult, Chromatography, Liquid, Humans, Male, Reference Standards, Testosterone, Ultrafiltration, Serum, Tandem Mass Spectrometry, Limit of Detection

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