Cited by 965Epigenetics and DNA MethylationMuscle Physiology and DisordersGenomics and Chromatin Dynamics

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Acute Exercise Remodels Promoter Methylation in Human Skeletal Muscle

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Barrès, Romain, Yan, Jie, Egan, Brendan, Treebak, Jonas Thue, Rasmussen, Morten, Fritz, Tomas, Caidahl, Kenneth, Krook, Anna, O'Gorman, Donal J., Zierath, Juleen R.·2012·Cell Metabolism

DOI 10.1016/j.cmet.2012.01.001

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Abstract

DNA methylation is a covalent biochemical modification controlling chromatin structure and gene expression. Exercise elicits gene expression changes that trigger structural and metabolic adaptations in skeletal muscle. We determined whether DNA methylation plays a role in exercise-induced gene expression. Whole genome methylation was decreased in skeletal muscle biopsies obtained from healthy sedentary men and women after acute exercise. Exercise induced a dose-dependent expression of PGC-1α, PDK4, and PPAR-δ, together with a marked hypomethylation on each respective promoter. Similarly, promoter methylation of PGC-1α, PDK4, and PPAR-δ was markedly decreased in mouse soleus muscles 45 min after ex vivo contraction. In L6 myotubes, caffeine exposure induced gene hypomethylation in parallel with an increase in the respective mRNA content. Collectively, our results provide evidence that acute gene activation is associated with a dynamic change in DNA methylation in skeletal muscle and suggest that DNA hypomethylation is an early event in contraction-induced gene activation.

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A tissue specific atlas of gene promoter DNA methylation variability and the clinical value of its assessment
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Background

Authors: Barrès, R., Yan, J., Egan, B., Treebak, J. T., Rasmussen, M., Fritz, T., Caidahl, K., Krook, A., O'Gorman, D. J., & Zierath, J. R.
Affiliations: University of Copenhagen (DK)
Karolinska University Hospital (SE)
Corresponding author: Zierath, Juleen R.
ORCID: Barrès, Romain
Yan, Jie
Egan, Brendan
Treebak, Jonas Thue
Rasmussen, Morten
Caidahl, Kenneth
Krook, Anna
Zierath, Juleen R.
Venue: Cell Metabolism
Publisher: Elsevier BV
Published: 2012-03
Volume: 15
Issue: 3
Pages: 405-411
ISSN: 1550-4131 (ISSN-L)
DOI: 10.1016/j.cmet.2012.01.001
OpenAlex: W2113728034
Cited by: 965
References: 32
Citations by year: 2026: 65 · 2025: 64 · 2024: 61 · 2023: 39 · 2022: 67 · 2021: 73 · 2020: 53 · 2019: 67 · 2018: 75 · 2017: 79 · 2016: 65 · 2015: 77 · 2014: 76 · 2013: 75 · 2012: 28
FWCI: 32.94
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 503 d)
Locations: Cell Metabolism (publishedVersion)
PubMed (publishedVersion)
Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords: DNA methylation, promoter hypomethylation, acute exercise, skeletal muscle, PGC-1α, PDK4, PPARδ, gene expression regulation, muscle contraction, caffeine, soleus muscle, L6 myotubes, epigenetic regulation
MeSH terms: Adult, Animals, Female, Humans, Male, Mice, Inbred C57BL, Exercise, Muscle, Skeletal, DNA Methylation, Mice, Young Adult

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Acute Exercise Remodels Promoter Methylation in Human Skeletal Muscle

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