Accelerated bone growth in vitro by the conjugation of BMP2 peptide with hydroxyapatite on titanium alloy
Abstract
Titanium alloys have been widely used in orthopedic practice due to their inherent bioactivity, however it is still insufficient to truly and reliably incorporate into living bone. In this work, polydopamine film was employed to induce the growth of hydroxyapatite (HA) on titanium alloy to enhance its osteoconductivity. Bone morphogenetic protein-2 (BMP2) peptide was absorbed into the HA particles for osteoinductivity. The precipitation of HA and the existence of BMP2 peptide were examined by X-ray diffraction, X-ray photoelectron spectroscopy and fluorescence microscopy. The dissolution of HA and the release of BMP2 peptide were monitored by measuring the concentrations of calcium ions and BMP2 peptide in phosphate buffered saline solution, respectively. The effect of BMP2 peptide incorporated into HA coating on bone growth was evaluated in vitro by cell culture tests, including cell attachment, alkaline phosphatase (ALP) activity, and gene expression. The results show that the HA particles grown on the substrate are mediated by the polydopamine film. The BMP2 peptide is distributed uniformly on HA-coated substrate and released in a sustained manner. Moreover, the conjunction of HA and BMP2 peptide increases cell adhesion, ALP activity and gene expression of osteogenic markers, which are potentially useful in the development of enhanced orthopedic medical devices.
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Compared to traditional surface modification approaches, PDA-assisted surface modification may remedy some drawbacks from traditional approaches.
“Compared to traditional surface modification approaches including physical deposition/absorption, chemical modification/grafting, and plasma technology, the PDA-assisted surface modification may remedy some drawbacks from traditional approaches [71].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [71]
The effect of polydopamine on osteoblast proliferation and calcium deposition is further strengthened in combination with growth factors.
“It has been reported that PDA enhances the proliferation and calcium deposition of osteoblast cells and the effect can be further strengthened in the combination of growth factors [71, 72].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [71]
Polydopamine enhances proliferation and calcium deposition of osteoblast cells.
“It has been reported that PDA enhances the proliferation and calcium deposition of osteoblast cells and the effect can be further strengthened in the combination of growth factors [71, 72].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [71]
PDA-assisted surface modification to Ti and Ti alloys offers osteoinductivity to bioinert metallic bone biosubstitutes.
“In addition, the PDA-assisted surface modification to Ti and Ti alloys can also offer the osteoinductivity and osteoconductivity to bioinert metallic bone biosubstitutes and enhance their bioactivities regarding the viability, proliferation, and differentiation of cell and material mineralization [23, 71, 72, 88].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [71]
PDA-assisted surface modification to Ti and Ti alloys enhances cell viability, proliferation, differentiation, and material mineralization.
“In addition, the PDA-assisted surface modification to Ti and Ti alloys can also offer the osteoinductivity and osteoconductivity to bioinert metallic bone biosubstitutes and enhance their bioactivities regarding the viability, proliferation, and differentiation of cell and material mineralization [23, 71, 72, 88].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [71]
PDA-assisted surface modification to Ti and Ti alloys offers osteoconductivity to bioinert metallic bone biosubstitutes.
“In addition, the PDA-assisted surface modification to Ti and Ti alloys can also offer the osteoinductivity and osteoconductivity to bioinert metallic bone biosubstitutes and enhance their bioactivities regarding the viability, proliferation, and differentiation of cell and material mineralization [23, 71, 72, 88].”
From Polydopamine-Assisted Surface Modification for Bone Biosubstitutes (2016) · cites this paper as [71]
Cites
Mussel‐Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite Crystallization
Ryu, J., Ku, S. H., Lee, H., & Park, C. B. (2010) · Advanced Functional Materials · doi:10.1002/adfm.200902347 · Cited by 778 · see citing works
The effect of VEGF functionalization of titanium on endothelial cells in vitro
Poh, C. K., Shi, Z., Lim, T. Y., Neoh, K. G., & Wang, W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2009.11.042 · Cited by 236 · see citing works
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Polydopamine-Assisted Surface Modification for Bone Biosubstitutes
Huang, S., Liang, N., Hu, Y., Zhou, X., & Abidi, N. (2016) · BioMed Research International · doi:10.1155/2016/2389895 · Cited by 97 · see citing works
Xu, Y., Li, H., Wu, J., Yang, Q., Jiang, D., & Qiao, B. (2018) · International Journal of Nanomedicine · doi:10.2147/ijn.s181137 · Cited by 42 · see citing works
- Authors
- Cai, Y., Wang, X., Poh, C. K., Tan, H. C., Soe, M. T., Zhang, S., & Wang, W.
- Affiliations
- National University of Singapore (SG)
- Nanyang Technological University (SG)
- Corresponding author
- Wang, Xiaoyan
- Wang, Wilson
- Venue
- Colloids and Surfaces B: Biointerfaces
- Publisher
- Elsevier BV
- Published
- 2014-04
- Volume
- 116
- Pages
- 681-686
- ISSN
- 0927-7765 (ISSN-L)
- OpenAlex
- W1967869386
- Cited by
- 44
- References
- 2
- Citations by year
- 2025: 2 · 2023: 3 · 2022: 1 · 2021: 3 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 6 · 2015: 7 · 2014: 1
- FWCI
- 2.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Colloids and Surfaces B Biointerfaces (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Physical Sciences › Materials Science › Materials Chemistry › Titanium Alloys Microstructure and Properties
- Keywords
- BMP-2, hydroxyapatite coating, titanium alloys, polydopamine films, osteoinduction, osteoconductivity, bone growth, alkaline phosphatase activity, osteogenic differentiation, orthopedic implants, sustained drug release
- MeSH terms
- Alkaline Phosphatase, Alloys, Bone Development, Cell Adhesion, Humans, Surface Properties, Titanium, Durapatite, Cell Line, Tumor, Bone Morphogenetic Protein 2
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