Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions
Abstract
Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1μm in length and 0.02-0.1μm in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology.
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Most DESs proposed so far in the literature have a hydrophilic character and are unstable in water, leading to separation of both components.
“Most of the DESs proposed so far in the open literature have a hydrophilic character and thus are unstable in water [30,31], leading to the separation of both components.”
From Development of hydrophobic deep eutectic solvents for extraction of pesticides from aqueous environments (2017) · cites this paper as [30]
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Self-citationMohammad-Zaheri, M., Mousavi-Jarrahi, Z., Ahmadi, S., Rezaeifar, F., Ghasemi, A., Ghahremani, M., Mamashli, F., Meratan, A. A., & Moosavi‐Movahedi, A. A. (2026) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.6c04016
- Authors
- Silva, N. H. C. S., Pinto, R. J. B., Freire, C. S. R., & Marrucho, I. M.
- Affiliations
- Instituto de Tecnologia Química e Biológica António Xavier (PT)
- University of Aveiro (PT)
- University of Lisbon (PT)
- Corresponding author
- Marrucho, Isabel M.
- Venue
- Colloids and Surfaces B: Biointerfaces
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 147
- Pages
- 36-44
- ISSN
- 0927-7765 (ISSN-L)
- OpenAlex
- W2465203846
- Cited by
- 47
- References
- 83
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 4 · 2020: 7 · 2019: 2 · 2018: 4 · 2017: 3
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Colloids and Surfaces B Biointerfaces (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
- Funders
- Fundação para a Ciência e Tecnologia (SFRH/BD/85690/2012)
- Research Unit GREEN-it (UID/Multi/04551/2013)
- CICECO-Aveiro Institute of Materials (FCTUID/CTM/50011/2013)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- hen egg white lysozyme, deep eutectic solvents, acetic acid, protein fibrillation, amyloid fibrils, nanofiber morphology, pH and temperature effects
- MeSH terms
- Animals, Chickens, Female, Hydrogen-Ion Concentration, Kinetics, Models, Molecular, Muramidase, Solvents, Tissue Engineering, Tissue Scaffolds, Nanofibers
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