Cited by 47Supramolecular Self-Assembly in MaterialsProtein purification and stabilityAlzheimer's disease research and treatments

Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions

Silva, Nuno H.C.S., Pinto, Ricardo J.B., Freire, Carmen S.R., Marrucho, Isabel M.·2016·Colloids and Surfaces B: Biointerfaces

Abstract

Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1μm in length and 0.02-0.1μm in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology.

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Crossref · 10.1016/j.colsurfb.2016.07.005

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Authors
Silva, N. H. C. S., Pinto, R. J. B., Freire, C. S. R., & Marrucho, I. M.
Affiliations
  • Instituto de Tecnologia Química e Biológica António Xavier (PT)
  • University of Aveiro (PT)
  • University of Lisbon (PT)
Corresponding author
Marrucho, Isabel M.
Venue
Colloids and Surfaces B: Biointerfaces
Publisher
Elsevier BV
Published
2016-11
Volume
147
Pages
36-44
ISSN
0927-7765 (ISSN-L)
OpenAlex
W2465203846
Cited by
47
References
83
Citations by year
2026: 3 · 2025: 5 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 4 · 2020: 7 · 2019: 2 · 2018: 4 · 2017: 3
FWCI
1.10
Type
journal article
Language
en
Open access
closed
Locations
  • Colloids and Surfaces B Biointerfaces (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
Funders
  • Fundação para a Ciência e Tecnologia (SFRH/BD/85690/2012)
  • Research Unit GREEN-it (UID/Multi/04551/2013)
  • CICECO-Aveiro Institute of Materials (FCTUID/CTM/50011/2013)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
hen egg white lysozyme, deep eutectic solvents, acetic acid, protein fibrillation, amyloid fibrils, nanofiber morphology, pH and temperature effects
MeSH terms
Animals, Chickens, Female, Hydrogen-Ion Concentration, Kinetics, Models, Molecular, Muramidase, Solvents, Tissue Engineering, Tissue Scaffolds, Nanofibers

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