Season-long expression of Cry1Ac and Cry2Ab proteins in Bollgard II cotton in Australia
Abstract
Bollgard II cotton has been grown commercially in Australia since 2003 for control of the primary target species Helicoverpa armigera (Hübner) and Helicoverpa punctigera (Wallengren) Larvae of both species have been reported to survive at low frequencies on Bollgard II with larvae >8 mm recorded in between 7 and 18% of the area planted to Bollgard II cotton between 2005/06 and 2007/08. F1 and F2 tests have shown that this is not due to the presence of resistance genes in the surviving larvae. To understand if fluctuations in the expression of the Cry proteins in Bollgard II allow some larvae to survive, plant tissue samples were taken from five Bollgard II cultivars throughout the growing season within fields and from different farms within a production region between 2007 and 2010. The data indicate that the expression of both Cry proteins is similar to the known resistance-monitoring diagnostic concentrations and relatively uniform between fields within a farm and between farms within a region, with less than one-third of the tests at this level of variation being significant. However, there were intra-seasonal changes in expression of both Cry proteins and differences in expression between plant structures and between cultivars for both Cry proteins. Further work is needed to establish if this variation in Cry protein content in Bollgard II cotton affects the control of Helicoverpa spp. in the field or whether plant-physiological and pest-behavioural factors underlie the occasional occurrence of Helicoverpa larval survival on Bollgard II cotton.
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Concentrations of Bt proteins can vary considerably in different plant tissues, across developmental stages, and among different Bt proteins and transformation events.
“However, concentrations can vary considerably in different plant tissues, across different developmental stages, and among different Bt proteins and transformation events (Eisenring et al., 2017;Knight et al., 2013;Nguyen andJehle 2007, 2009;Obrist et al., 2006a;Svobodová et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Knight et al., 2013
Cites
Experimental Design and Data Analysis for Biologists
Quinn, G. P., & Keough, M. J. (2002) · doi:10.1017/cbo9780511806384 · Cited by 10,674 · see citing works
Experimental Design and Data Analysis for Biologists
Reid, J. R. W. (2003) · Austral Ecology · doi:10.1046/j.1442-9993.2003.01318.x · Cited by 1,542 · see citing works
Gould, F. L., Anderson, A., Jones, A., Sumerford, D. V., Heckel, D. G., López, J. D., Micinski, S., Leonard, R., & Laster, M. L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.8.3519 · Cited by 330 · see citing works
F2 Screen for Rare Resistance Alleles
Andow, D. A., & Alstad, D. N. (1998) · Journal of Economic Entomology · doi:10.1093/jee/91.3.572 · Cited by 308 · see citing works
Greenplate, J. T. (1999) · Journal of Economic Entomology · doi:10.1093/jee/92.6.1377 · Cited by 245 · see citing works
Variability of Endotoxin Expression in Bt Transgenic Cotton
Dong, H., & Li, W. J. (2006) · Journal of Agronomy and Crop Science · doi:10.1111/j.1439-037x.2006.00240.x · Cited by 168 · see citing works
Adamczyk, J. J., Adams, L. C., & Hardee, D. D. (2001) · Journal of Economic Entomology · doi:10.1603/0022-0493-94.6.1589 · Cited by 153 · see citing works
Olsen, K., Daly, J. C., Holt, H. E., & Finnegan, E. J. (2005) · Journal of Economic Entomology · doi:10.1603/0022-0493-98.3.1007 · Cited by 152 · see citing works
Adamczyk, J. J., Hardee, D. D., Adams, L. C., & Sumerford, D. V. (2001) · Journal of Economic Entomology · doi:10.1603/0022-0493-94.1.284 · Cited by 149 · see citing works
Liao, C., Heckel, D. G., & Akhurst, R. J. (2002) · Journal of Invertebrate Pathology · doi:10.1016/s0022-2011(02)00035-6 · Cited by 148 · see citing works
Gore, J., Leonard, B. R., & Adamczyk, J. J. (2001) · Journal of Economic Entomology · doi:10.1603/0022-0493-94.6.1445 · Cited by 136 · see citing works
The effect of high temperature on the insecticidal properties of Bt Cotton
CHEN, D., Ye, G., YANG, C., CHEN, Y., & Wu, Y. (2004) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2004.04.004 · Cited by 123 · see citing works
Stone, T. B., & Sims, S. R. (1993) · Journal of Economic Entomology · doi:10.1093/jee/86.4.989 · Cited by 114 · see citing works
Greenplate, J. T., Mullins, J. W., Penn, S. R., Dahm, A., Reich, B. J., Osborn, J. A., Rahn, P. R., Ruschke, L. G., & Shappley, Z. W. (2003) · Journal of Applied Entomology · doi:10.1046/j.1439-0418.2003.00766.x · Cited by 113 · see citing works
Behavior of Bollworm (Lepidoptera: Noctuidae) Larvae on Genetically Engineered Cotton
Gore, J., Leonard, B. R., CHURCH, G. E., & Cook, D. R. (2002) · Journal of Economic Entomology · doi:10.1603/0022-0493-95.4.763 · Cited by 98 · see citing works
Mahon, R. J., Olsen, K., Downes, S. J., & Addison, S. J. (2007) · Journal of Economic Entomology · doi:10.1603/0022-0493(2007)100[1844:foacrt]2.0.co;2 · Cited by 96 · see citing works
Luo, Z., Dong, H., Li, W., Ming, Z., & Zhu, Y. (2008) · Crop Protection · doi:10.1016/j.cropro.2008.06.006 · Cited by 82 · see citing works
Adamczyk, J. J., Holloway, J. W., CHURCH, G. E., Leonard, B. R., & Graves, J. B. (1998) · Journal of Economic Entomology · doi:10.1093/jee/91.2.539 · Cited by 62 · see citing works
Bird, L. J., & Akhurst, R. J. (2006) · Journal of Invertebrate Pathology · doi:10.1016/j.jip.2006.08.005 · Cited by 55 · see citing works
Gore, J., Adamczyk, J. J., & Blanco, C. A. (2005) · Journal of Economic Entomology · doi:10.1603/0022-0493-98.1.88 · Cited by 52 · see citing works
Deployment and Impact of Transgenic Bt Cotton in Australia
Fitt, G. P. (2003) · Springer eBooks · doi:10.1007/978-1-4615-0177-0_8 · Cited by 43 · see citing works
The Economic and Environmental Impacts of Agbiotech. A Global Perspective
Bolton, S. (2003) · Ecological Economics · doi:10.1016/j.ecolecon.2003.07.010 · Cited by 36 · see citing works
Lu, B., Downes, S. J., Wilson, L., Gregg, P. C., Knight, K., Kauter, G., & McCorkell, B. (2011) · Entomologia Experimentalis et Applicata · doi:10.1111/j.1570-7458.2011.01135.x · Cited by 36 · see citing works
Sims, S. R., Pershing, J. C., & Reich, B. J. (1996) · Journal of Entomological Science · doi:10.18474/0749-8004-31.3.340 · Cited by 27 · see citing works
Addison, S. J., & Rogers, D. J. (2010) · Journal of Economic Entomology · doi:10.1603/ec09369 · Cited by 24 · see citing works
Singh, G., Rup, P. J., & Koul, O. (2008) · Journal of Insect Behavior · doi:10.1007/s10905-008-9139-y · Cited by 15 · see citing works
Flagged first, then by citations
2 of the 28 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231 · see citing works
“considerably in different plant tissues, across different developmental stages, and among different Bt proteins and transformation events (Eisenring et al., 2017; Knight et al., 2013; Nguyen and Jehle 2007, 2009; Obrist et al., 2006a; Svobodová et al., 2017).”
BackgroundNaik, V. C. B., Kumbhare, S., Kranthi, S., Satija, U., & Kranthi, K. R. (2018) · Pest Management Science · doi:10.1002/ps.5038 · Cited by 162 · see citing works
Behavior as a mechanism of insecticide resistance: evaluation of the evidence
Zalucki, M. P., & Furlong, M. J. (2017) · Current Opinion in Insect Science · doi:10.1016/j.cois.2017.05.006 · Cited by 117 · see citing works
Liu, L., Gao, M., Song, Y., Liu, S., Wu, Y., Carrière, Y., & Yang, Y. (2016) · Evolutionary Applications · doi:10.1111/eva.12438 · Cited by 39 · see citing works
Meissle, M., & Romeis, J. (2017) · Insect Science · doi:10.1111/1744-7917.12468 · Cited by 33 · see citing works
Carrière, Y., Degain, B. A., Unnithan, G. C., Harpold, V. S., Li, X., & Tabashnik, B. E. (2019) · Journal of Economic Entomology · doi:10.1093/jee/toz236 · Cited by 28 · see citing works
Fluctuating temperatures influence the susceptibility of pest insects to biological control agents
Ghazanfar, M. U., Hagenbucher, S., Romeis, J., Grabenweger, G., & Meissle, M. (2020) · Journal of Pest Science · doi:10.1007/s10340-020-01215-9 · Cited by 26 · see citing works
Knight, K., Head, G., & Rogers, D. J. (2021) · Pest Management Science · doi:10.1002/ps.6490 · Cited by 24 · see citing works
Multitrophic Cry-protein flow in a dual-gene Bt-cotton field
Eisenring, M., Romeis, J., Naranjo, S. E., & Meissle, M. (2017) · Agriculture, Ecosystems & Environment · doi:10.1016/j.agee.2017.07.009 · Cited by 17 · see citing works
Luong, T. T. A., Zalucki, M. P., Perkins, L. E., & Downes, S. J. (2017) · Austral Entomology · doi:10.1111/aen.12265 · Cited by 17 · see citing works
Bt insecticidal efficacy variation and agronomic regulation in Bt cotton
Liu, Z., H, M. A. A. E., Huimin, W. U., Zhou, M., ZHANG, X., Chen, Y., & Chen, D. (2019) · Journal of Cotton Research · doi:10.1186/s42397-019-0042-1 · Cited by 16 · see citing works
Bt, Not a Threat to Propylea japonica
Zhao, C., Wu, L., Luo, J., Niu, L., Wang, C., Zhu, X., Wang, L., Zhao, P., Zhang, S., & Cui, J. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.00758 · Cited by 13 · see citing works
Knight, K., Head, G., & Rogers, J. (2015) · Crop Protection · doi:10.1016/j.cropro.2015.10.013 · Cited by 10 · see citing works
Saleem, M. F., Shakoor, A., Shahid, M. A., Cheema, M., Shakeel, A., Shahid, M., Tahir, M. U., & Bilal, M. F. (2018) · Industrial Crops and Products · doi:10.1016/j.indcrop.2018.07.084 · Cited by 9 · see citing works
Holman, S., Grundy, P., Spafford, H., & Furlong, M. J. (2025) · Journal of Economic Entomology · doi:10.1093/jee/toaf089 · Cited by 8 · see citing works
Chen, Y., Meissle, M., Xue, J., Zhang, N., Ma, S., Guo, A., Liu, B., Peng, Y., Song, X., Yang, Y., et al. (2023) · Plants · doi:10.3390/plants12234057 · Cited by 6 · see citing works
Tang, J., Lu, J., Zhang, C., Yu, S., Ding, Z., Soe, E. T., & Liang, G. (2023) · Journal of Pest Science · doi:10.1007/s10340-023-01646-0 · Cited by 6 · see citing works
Inhibition of protein degradation increases the Bt protein concentration in Bt cotton
Liu, Y., Li, H., Chen, Y., Tambel, L. I. M., Liu, Z., Wu, S., Sun, S., Zhang, X., & De-hua, C. H. E. N. (2023) · Journal of Integrative Agriculture · doi:10.1016/j.jia.2023.06.033 · Cited by 6 · see citing works
Dhanaraj, A. L., Willse, A., & Kamath, S. P. (2018) · Transgenic Research · doi:10.1007/s11248-018-0102-1 · Cited by 6 · see citing works
Kim, Y.-J., Kloos, S., Romeis, J., & Meissle, M. (2020) · Journal of Pest Science · doi:10.1007/s10340-020-01260-4 · Cited by 6 · see citing works
Zhou, M., Chen, C., Tambel, L. I. M., Chen, Y., ZHANG, X., Chen, Y., & Chen, D. (2021) · Journal of Cotton Research · doi:10.1186/s42397-021-00084-6 · Cited by 3 · see citing works
Khakwani, K., CENGIZ, R., NASEER, S., ASIF, M., & Sarwar, G. (2022) · Applied Ecology and Environmental Research · doi:10.15666/aeer/2002_11991213 · Cited by 3 · see citing works
Holman, S., Grundy, P., Spafford, H., & Furlong, M. J. (2026) · Entomologia Experimentalis et Applicata · doi:10.1111/eea.70086 · Cited by 2 · see citing works
Response of the Propylea japonica Microbiota to Treatment with Cry1B Protein
Diao, F., Li, Y., Gao, X., Luo, J., Zhu, X., Wang, L., Zhang, K., Li, D., Ji, J., & Cui, J. (2023) · Genes · doi:10.3390/genes14112008 · Cited by 2 · see citing works
Little, N. S., Paulk, R. T., Elkins, B. H., & Allen, K. C. (2024) · Southwestern Entomologist · doi:10.3958/059.049.0215 · Cited by 1 · see citing works
Keim, D., Chaudhry, M. R., Kranthi, S., & Ethridge, D. (2021) · Springer eBooks · doi:10.1007/978-3-030-64504-5_17
Naik, V. C. B., Saonerkar, T. D., Chandrashekar, N., Pusadkar, P., Kranthi, S., Verma, P., Kumbhare, S., Gokte‐Narkhedkar, N., & Prasad, Y. G. (2022) · Journal of Environmental Biology · doi:10.22438/jeb/43/5/mrn-2069
Shrilakshmi, R. G., & Ss, U. (2024) · International Journal of Advanced Biochemistry Research · doi:10.33545/26174693.2024.v8.i3b.686
Shrilakshmi, R. G., & Udikeri, S. S. (2024) · Journal of Farm Sciences · doi:10.61475/jfs.2023.v36i3.04
Kaur, J., & Aggarwal, N. (2024) · Research Square · doi:10.21203/rs.3.rs-4443363/v1
Little, N. S., Paulk, R. T., Elkins, B. H., Mullen, R. M., & Allen, K. C. (2026) · Crop Protection · doi:10.1016/j.cropro.2026.107543
Holman, S. R., Grundy, P., Spafford, H., & Furlong, M. J. (2026) · Journal of Economic Entomology · doi:10.1093/jee/toag187
- Authors
- Knight, K., Head, G., & Rogers, J.
- Affiliations
- Monsanto (United States) (US)
- Corresponding author
- Knight, Kristen
- ORCID
- Head, Graham
- Venue
- Crop Protection
- Publisher
- Elsevier BV
- Published
- 2013-02
- Volume
- 44
- Pages
- 50-58
- ISSN
- 0261-2194 (ISSN-L)
- OpenAlex
- W2069789155
- Cited by
- 32
- References
- 28
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 1 · 2015: 1
- FWCI
- 0.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Crop Protection (publishedVersion)
- Funders
- Commonwealth Scientific and Industrial Research Organisation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Keywords
- Cry1Ac, Cry2Ab, Bt cotton, Helicoverpa armigera, Helicoverpa punctigera, insect resistance, larval survival, intraseasonal variability, plant tissue, cultivar differences, resistance monitoring
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