Multitrophic Cry-protein flow in a dual-gene Bt-cotton field
Open accessAbstract
The transfer of plant-produced insecticidal Cry-proteins in the arthropod food web can be affected by a number of environmental and ecological factors. Despite this fact, most studies documenting multitrophic Cry-protein acquisition patterns in arthropods are conducted under controlled conditions whereas the number of field studies is limited. Such field studies, however, are valuable to understand multitrophic allocation dynamics of Cry-proteins under ecologically realistic conditions and are therefore important for the interpretation and design of laboratory hazard studies on genetically engineered (GE) crops. We thus sampled arthropods and plant structures in a field planted with GE dual-gene cotton plants producing the Cry-proteins Cry1Ac and Cry2Ab from Bacillus thuringiensis Berliner over the growing season. Cry-protein concentrations in field-collected plants, herbivores, and predators were quantified and compared with arthropods subjected to tri-trophic laboratory feeding assays. Both, field studies and laboratory assays showed that Cry-protein concentrations strongly decreased with increasing trophic level to values mostly below the detection limit in predators. Under field conditions, in-planta Cry-protein concentrations varied between plant structures and over the season. Concentrations in arthropods were mainly associated with feeding mode, feeding location on the plant, and to a lesser degree seasonality. Compared to plants, arthropods showed lower Cry2Ab:Cry1Ac ratios indicating that Cry2Ab might be less stable than Cry1Ac. Of all predators collected in the field study, we measured highest Cry-protein levels in jumping and crab spiders, predatory flies and some predatory hemipterans. This emphasizes the relevance of these groups for the risk assessment of GE cotton.
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Concentrations of Bt proteins can vary considerably in different plant tissues, across developmental stages, and among different Bt proteins and transformation events.
“However, concentrations can vary considerably in different plant tissues, across different developmental stages, and among different Bt proteins and transformation events (Eisenring et al., 2017;Knight et al., 2013;Nguyen andJehle 2007, 2009;Obrist et al., 2006a;Svobodová et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Eisenring et al., 2017
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Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231 · see citing works
“…is supported by evidence from ELISA measurements of field collected arthropods from Bt maize (Harwood et al., 2005; Meissle and Romeis, 2009a; Obrist et al., 2006a, Peterson et al., 2009), cotton (Eisenring et al., 2017; Torres et al., 2006), soybean (Yu et al., 2014), and rice (Li et al., 2017b).”
BackgroundSelf-citation
- Authors
- Eisenring, M., Romeis, J., Naranjo, S. E., & Meissle, M.
- Affiliations
- Agroscope (CH)
- Agricultural Research Service (US)
- Corresponding author
- Meissle, Michael
- Venue
- Agriculture, Ecosystems & Environment
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 247
- Pages
- 283-289
- ISSN
- 0167-8809 (ISSN-L)
- OpenAlex
- W2735191336
- Cited by
- 17
- References
- 41
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 4
- FWCI
- 2.96
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (am, 314 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Agriculture Ecosystems & Environment (acceptedVersion)
- Funders
- Swiss National Science Foundation (31003A-149794)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Keywords
- Cry proteins, Bt cotton, Cry1Ac, Cry2Ab, Bacillus thuringiensis, arthropod food webs, trophic levels, predators, herbivores, Salticidae, Thomisidae, predatory flies
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