Cited by 83Zebrafish Biomedical Research ApplicationsNeonatal and Maternal InfectionsBacterial Infections and Vaccines

Adult zebrafish model of bacterial meningitis in Streptococcus agalactiae infection

Patterson, Hayley, Saralahti, Anni, Parikka, Mataleena, Dramsi, Shaynoor, Trieu-Cuot, Patrick, Poyart, Claire, Rounioja, Samuli, Rämet, Mika·2012·Developmental & Comparative Immunology

Abstract

Streptococcus agalactiae (Group B Streptococcus, GBS) is the major cause of severe bacterial disease and meningitis in newborns. The zebrafish (Danio rerio) has recently emerged as a valuable and powerful vertebrate model for the study of human streptococcal infections. In the present study we demonstrate that adult zebrafish are susceptible to GBS infection through the intraperitoneal and intramuscular routes of infection. Following intraperitoneal challenge with GBS, zebrafish developed a fulminant infection 24-48 h post-injection, with signs of pathogenesis including severe inflammation at the injection site and meningoencephalitis. Quantification of blood and brain bacterial load confirmed that GBS is capable of replicating in the zebrafish bloodstream and penetrating the blood-brain barrier, resulting in the induction of host inflammatory immune responses in the brain. Additionally, we show that GBS mutants previously described as avirulent in the mice model, have an impaired ability to cause meningitis in this new in vivo model. Taken together, our data demonstrates that adult zebrafish may be used as a bacterial meningitis model as a means for deciphering the pathogenesis and development of invasive GBS disease.

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Crossref · 10.1016/j.dci.2012.07.007

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Authors
Patterson, H., Saralahti, A., Parikka, M., Dramsi, S., Trieu-Cuot, P., Poyart, C., Rounioja, S., & Rämet, M.
Affiliations
  • Tampere University (FI)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
Rounioja, Samuli
Venue
Developmental & Comparative Immunology
Publisher
Elsevier BV
Published
2012-11
Volume
38
Issue
3
Pages
447-455
ISSN
0145-305X (ISSN-L)
OpenAlex
W2055555815
Cited by
83
References
55
Citations by year
2026: 5 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 7 · 2021: 5 · 2020: 10 · 2019: 8 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 10 · 2014: 7 · 2013: 2
FWCI
2.88
Type
journal article
Language
en
Open access
closed
Locations
  • Developmental & Comparative Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
  • Pirkanmaan Sairaanhoitopiiri
  • Biocenter Finland
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
Keywords
Streptococcus agalactiae, bacterial meningitis, zebrafish model, adult zebrafish, meningoencephalitis, blood-brain barrier, neuroinflammation, invasive streptococcal infection, peritoneal infection, intramuscular infection, bacterial load
MeSH terms
Animals, Blood-Brain Barrier, Brain, Disease Models, Animal, Meningoencephalitis, Streptococcal Infections, Streptococcus agalactiae, Zebrafish, Meningitis, Bacterial, Bacterial Load

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