Adult zebrafish model of bacterial meningitis in Streptococcus agalactiae infection
Abstract
Streptococcus agalactiae (Group B Streptococcus, GBS) is the major cause of severe bacterial disease and meningitis in newborns. The zebrafish (Danio rerio) has recently emerged as a valuable and powerful vertebrate model for the study of human streptococcal infections. In the present study we demonstrate that adult zebrafish are susceptible to GBS infection through the intraperitoneal and intramuscular routes of infection. Following intraperitoneal challenge with GBS, zebrafish developed a fulminant infection 24-48 h post-injection, with signs of pathogenesis including severe inflammation at the injection site and meningoencephalitis. Quantification of blood and brain bacterial load confirmed that GBS is capable of replicating in the zebrafish bloodstream and penetrating the blood-brain barrier, resulting in the induction of host inflammatory immune responses in the brain. Additionally, we show that GBS mutants previously described as avirulent in the mice model, have an impaired ability to cause meningitis in this new in vivo model. Taken together, our data demonstrates that adult zebrafish may be used as a bacterial meningitis model as a means for deciphering the pathogenesis and development of invasive GBS disease.
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Adult zebrafish have been used to model Streptococcus iniae and GBS.
“Adult zebrafish have been used to model Streptococcus iniae and, recently, GBS (32,33).”
From Bacterial induction of Snail1 contributes to blood-brain barrier disruption (2015) · cites this paper as [32]
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- Authors
- Patterson, H., Saralahti, A., Parikka, M., Dramsi, S., Trieu-Cuot, P., Poyart, C., Rounioja, S., & Rämet, M.
- Affiliations
- Tampere University (FI)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Rounioja, Samuli
- Venue
- Developmental & Comparative Immunology
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 38
- Issue
- 3
- Pages
- 447-455
- ISSN
- 0145-305X (ISSN-L)
- OpenAlex
- W2055555815
- Cited by
- 83
- References
- 55
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 7 · 2021: 5 · 2020: 10 · 2019: 8 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 10 · 2014: 7 · 2013: 2
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental & Comparative Immunology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Pirkanmaan Sairaanhoitopiiri
- Biocenter Finland
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Keywords
- Streptococcus agalactiae, bacterial meningitis, zebrafish model, adult zebrafish, meningoencephalitis, blood-brain barrier, neuroinflammation, invasive streptococcal infection, peritoneal infection, intramuscular infection, bacterial load
- MeSH terms
- Animals, Blood-Brain Barrier, Brain, Disease Models, Animal, Meningoencephalitis, Streptococcal Infections, Streptococcus agalactiae, Zebrafish, Meningitis, Bacterial, Bacterial Load
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