The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization
Open accessAbstract
Summary The earliest aspects of human embryogenesis remain mysterious. To model patterning events in the human embryo we used colonies of human embryonic stem cells (hESCs) grown on micropatterned substrate and differentiated with BMP4. These gastruloids recapitulate the embryonic arrangement of the mammalian germ layers and provide an assay to assess the structural and signaling mechanisms patterning the human gastrula. Structurally, high-density hESCs lateralize their TGF-β receptors to their lateral side in the center of the colony, while maintaining apical localization of receptors at the edge. This relocalization insulates cells at the center from apically applied ligands while maintaining response to basally presented ones. Additionally, BMP4 directly induces the expression of its own inhibitor, Noggin, generating a reaction-diffusion mechanism that underlies patterning. We develop a quantitative model that integrates edge sensing and inhibitors, to predict human fate positioning in gastruloids, and potentially the human embryo.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A method to recapitulate early embryonic spatial patterning in human embryonic stem cells
Warmflash, A., Sorre, B., Etoc, F., Siggia, E. D., & Brivanlou, A. H. (2014) · Nature Methods · doi:10.1038/nmeth.3016 · Cited by 885
Self-citationMaking a commitment: cell lineage allocation and axis patterning in the early mouse embryo
Arnold, S. J., & Robertson, E. J. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2618 · Cited by 816 · cited 6×
“A uthor M anuscript A uthor M anuscript A uthor M anuscript A uthor M anuscript NOGGIN as a direct BMP target; CHX cyclohexamide, SB and IWP2 respectively inhibit Activin/Nodal and Wnt signaling.”
BackgroundInfluentialCellular dynamics in the early mouse embryo: from axis formation to gastrulation
Nowotschin, S., & Hadjantonakis, A.-K. (2010) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2010.05.008 · Cited by 79
Cited by
Progress and potential in organoid research
Rossi, G., Manfrin, A., & Lutolf, M. P. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0051-9 · Cited by 1,182
Engineering Stem Cell Self-organization to Build Better Organoids
Brassard, J. A., & Lutolf, M. P. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.05.005 · Cited by 373
Mechanochemical Feedback Loops in Development and Disease
Hannezo, E., & Heisenberg, C.-P. (2019) · Cell · doi:10.1016/j.cell.2019.05.052 · Cited by 353
BMP4 patterns Smad activity and generates stereotyped cell fate organization in spinal organoids
Duval, N., Vaslin, C., Barata, T. C., Frarma, Y., Contremoulins, V., Baudin, X., Nedelec, S., & Ribes, V. C. (2019) · Development · doi:10.1242/dev.175430 · Cited by 93
Kim, Y., Jang, H., Seo, K., Kim, J. H., Lee, B., Cho, H. M., Kim, H. J., Yang, E., Kim, H., Gim, J.-A., et al. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.11.005 · Cited by 33
Metadata
- Authors
- Etoc, F., Metzger, J., Ruzo, A., Kirst, C., Yoney, A., Ozair, M. Z., Brivanlou, A. H., & Siggia, E. D.
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 39
- Issue
- 3
- Pages
- 302-315
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2535856472
- Cited by
- 343
- References
- 3
- FWCI
- 14.60
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
