The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Large-Scale Analysis of CRISPR/Cas9 Cell-Cycle Knockouts Reveals the Diversity of p53-Dependent Responses to Cell-Cycle Defects
Open accessAbstract
SUMMARY Defining the genes that are essential for cellular proliferation is critical for understanding organismal development and identifying high-value targets for disease therapies. However, the requirements for cell-cycle progression in human cells remain incompletely understood. To elucidate the consequences of acute and chronic elimination of cell-cycle proteins, we generated and characterized inducible CRISPR/Cas9 knockout human cell lines targeting 209 genes involved in diverse cell-cycle processes. We performed single-cell microscopic analyses to systematically establish the effects of the knockouts on subcellular architecture. To define variations in cell-cycle requirements between cultured cell lines, we generated knockouts across cell lines of diverse origins. We demonstrate that p53 modulates the phenotype of specific cell-cycle defects through distinct mechanisms, depending on the defect. This work provides a resource to broadly facilitate robust and long-term depletion of cell-cycle proteins and reveals insights into the requirements for cell-cycle progression.
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Cites
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Self-citationJoint single-cell measurements of nuclear proteins and RNA in vivo
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Metadata
- Authors
- McKinley, K. L., & Cheeseman, I. M.
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2017-02
- Volume
- 40
- Issue
- 4
- Pages
- 405-420.e2
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2588526630
- Cited by
- 172
- References
- 10
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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