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Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation

Roberts, Melissa A., Deol, Kirandeep K., Mathiowetz, Alyssa J., Lange, Mike, Leto, Dara E., Stevenson, Julian, Hashemi, Sayed Hadi, Morgens, David W., Easter, Emilee, Heydari, Kartoosh, Nalls, Mike A., Bassik, Michael C., Kampmann, Martin, Kopito, Ron R., Faghri, Faraz, Olzmann, James A.·2023·Developmental Cell
DOI 10.1016/j.devcel.2023.07.001
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  1. Olzmann, 2019, Dynamics and functions of lipid droplets, Nat. Rev. Mol. Cell Biol., 20, 137, 10.1038/s41580-018-0085-z · DOI · Paper page
  2. Walther, 2017, Lipid droplet biogenesis, Annu. Rev. Cell Dev. Biol., 33, 491, 10.1146/annurev-cellbio-100616-060608 · DOI
  3. Renne, 2020, Lipid droplet biogenesis: a mystery “unmixing”?, Semin. Cell Dev. Biol., 108, 14, 10.1016/j.semcdb.2020.03.001 · DOI
  4. Thiam, 2021, Lipid droplet nucleation, Trends Cell Biol., 31, 108, 10.1016/j.tcb.2020.11.006 · DOI
  5. Bersuker, 2017, Establishing the lipid droplet proteome: mechanisms of lipid droplet protein targeting and degradation, Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1862, 1166, 10.1016/j.bbalip.2017.06.006 · DOI
  6. Krahmer, 2013, Protein correlation profiles identify lipid droplet proteins with high confidence, Mol. Cell. Proteomics, 12, 1115, 10.1074/mcp.M112.020230 · DOI
  7. Liu, 2004, Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic, J. Biol. Chem., 279, 3787, 10.1074/jbc.M311945200 · DOI
  8. Roberts, 2020, Protein quality control and lipid droplet metabolism, Annu. Rev. Cell Dev. Biol., 36, 115, 10.1146/annurev-cellbio-031320-101827 · DOI
  9. Kimmel, 2016, The perilipins: major cytosolic lipid droplet-associated proteins and their roles in cellular lipid storage, mobilization, and systemic homeostasis, Annu. Rev. Nutr., 36, 471, 10.1146/annurev-nutr-071813-105410 · DOI
  10. Najt, 2022, Perilipins at a glance, J. Cell Sci., 135, jcs259501, 10.1242/jcs.259501 · DOI
  11. Orlicky, 2019, Perilipin-2 promotes obesity and progressive fatty liver disease in mice through mechanistically distinct hepatocyte and extra-hepatocyte actions, J. Physiol., 597, 1565, 10.1113/JP277140 · DOI
  12. Griffin, 2021, Integrated action of autophagy and adipose tissue triglyceride lipase ameliorates diet-induced hepatic steatosis in liver-specific PLIN2 knockout mice, Cells, 10, 1016, 10.3390/cells10051016 · DOI
  13. Najt, 2016, Liver-specific loss of perilipin 2 alleviates diet-induced hepatic steatosis, inflammation, and fibrosis, Am. J. Physiol. Gastrointest. Liver Physiol., 310, G726, 10.1152/ajpgi.00436.2015 · DOI
  14. Listenberger, 2007, Adipocyte differentiation-related protein reduces the lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover, J. Lipid Res., 48, 2751, 10.1194/jlr.M700359-JLR200 · DOI
  15. Faulkner, 2020, A single nucleotide polymorphism of PLIN2 is associated with nonalcoholic steatohepatitis and causes phenotypic changes in hepatocyte lipid droplets: a pilot study, Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1865, 158637, 10.1016/j.bbalip.2020.158637 · DOI
  16. Magné, 2013, The minor allele of the missense polymorphism Ser251Pro in perilipin 2 (PLIN2) disrupts an α-helix, affects lipolysis, and is associated with reduced plasma triglyceride concentration in humans, FASEB J., 27, 3090, 10.1096/fj.13-228759 · DOI
  17. Sentinelli, 2016, The perilipin 2 (PLIN2) gene Ser251Pro missense mutation is associated with reduced insulin secretion and increased insulin sensitivity in Italian obese subjects, Diabetes Metab. Res. Rev., 32, 550, 10.1002/dmrr.2751 · DOI
  18. Gluchowski, 2017, Lipid droplets and liver disease: from basic biology to clinical implications, Nat. Rev. Gastroenterol. Hepatol., 14, 343, 10.1038/nrgastro.2017.32 · DOI
  19. Straub, 2008, Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis, Hepatology, 47, 1936, 10.1002/hep.22268 · DOI
  20. Cruz, 2020, Lipid droplets: platforms with multiple functions in cancer hallmarks, Cell Death Dis., 11, 105, 10.1038/s41419-020-2297-3 · DOI
  21. Xu, 2019, Perilipin 2 and lipid droplets provide reciprocal stabilization, Biophys. Rep., 5, 145, 10.1007/s41048-019-0091-5 · DOI
  22. Kaushik, 2015, Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis, Nat. Cell Biol., 17, 759, 10.1038/ncb3166 · DOI
  23. Masuda, 2006, ADRP/adipophilin is degraded through the proteasome-dependent pathway during regression of lipid-storing cells, J. Lipid Res., 47, 87, 10.1194/jlr.M500170-JLR200 · DOI
  24. Nguyen, 2019, N-terminal acetylation and the N-end rule pathway control degradation of the lipid droplet protein PLIN2, J. Biol. Chem., 294, 379, 10.1074/jbc.RA118.005556 · DOI · Paper page
  25. Xu, 2005, Post-translational regulation of adipose differentiation-related protein by the ubiquitin/proteasome pathway, J. Biol. Chem., 280, 42841, 10.1074/jbc.M506569200 · DOI
  26. Morgens, 2016, Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes, Nat. Biotechnol., 34, 634, 10.1038/nbt.3567 · DOI
  27. Morgens, 2017, Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens, Nat. Commun., 8, 15178, 10.1038/ncomms15178 · DOI
  28. Dubreuil, 2020, Systematic identification of regulators of oxidative stress reveals non-canonical roles for peroxisomal import and the pentose phosphate pathway, Cell Rep., 30, 1417, 10.1016/j.celrep.2020.01.013 · DOI
  29. Han, 2017, Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions, Nat. Biotechnol., 35, 463, 10.1038/nbt.3834 · DOI
  30. Parnas, 2015, A genome-wide CRISPR screen in primary immune cells to dissect regulatory networks, Cell, 162, 675, 10.1016/j.cell.2015.06.059 · DOI · Paper page
  31. Bersuker, 2018, A proximity labeling strategy provides insights into the composition and dynamics of lipid droplet proteomes, Dev. Cell, 44, 97, 10.1016/j.devcel.2017.11.020 · DOI · Paper page
  32. Bailey, 2015, Antioxidant role for lipid droplets in a stem cell niche of drosophila, Cell, 163, 340, 10.1016/j.cell.2015.09.020 · DOI
  33. Jin, 2018, Reactive oxygen species induces lipid droplet accumulation in HepG2 cells by increasing perilipin 2 expression, Int. J. Mol. Sci., 19, 3445, 10.3390/ijms19113445 · DOI
  34. Liu, 2017, The glia-neuron lactate shuttle and elevated ROS Promote Lipid synthesis in neurons and lipid droplet accumulation in glia via APOE/D, Cell Metab., 26, 719, 10.1016/j.cmet.2017.08.024 · DOI · Paper page
  35. Nowinski, 2020, Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria, eLife, 9, e58041, 10.7554/eLife.58041 · DOI
  36. Kaushik, 2016, AMPK-dependent phosphorylation of lipid droplet protein PLIN2 triggers its degradation by CMA, Autophagy, 12, 432, 10.1080/15548627.2015.1124226 · DOI
  37. Gill, 2011, Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase, Cell Metab., 13, 260, 10.1016/j.cmet.2011.01.015 · DOI · Paper page
  38. Stevenson, 2016, Endoplasmic reticulum-associated degradation and lipid homeostasis, Annu. Rev. Nutr., 36, 511, 10.1146/annurev-nutr-071715-051030 · DOI
  39. Romanauska, 2018, The inner nuclear membrane is a metabolically active territory that generates nuclear lipid droplets, Cell, 174, 700, 10.1016/j.cell.2018.05.047 · DOI
  40. Ghosh, 2016, Ubiquitin ligase COP1 controls hepatic fat metabolism by targeting ATGL for degradation, Diabetes, 65, 3561, 10.2337/db16-0506 · DOI
  41. Olzmann, 2013, Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover, Proc. Natl. Acad. Sci. USA, 110, 1345, 10.1073/pnas.1213738110 · DOI
  42. Goo, 2017, Lipid droplet-associated hydrolase promotes lipid droplet fusion and enhances ATGL degradation and triglyceride accumulation, Sci. Rep., 7, 2743, 10.1038/s41598-017-02963-y · DOI
  43. Patel, 2014, Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis, Proc. Natl. Acad. Sci. USA, 111, 9163, 10.1073/pnas.1318791111 · DOI
  44. Listenberger, 2003, Triglyceride accumulation protects against fatty acid-induced lipotoxicity, Proc. Natl. Acad. Sci. USA, 100, 3077, 10.1073/pnas.0630588100 · DOI · Paper page
  45. Rambold, 2015, Fatty acid trafficking in starved cells: regulation by lipid droplet lipolysis, autophagy, and mitochondrial fusion dynamics, Dev. Cell, 32, 678, 10.1016/j.devcel.2015.01.029 · DOI · Paper page
  46. Huang, 2021, TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis, Cell Metab., 33, 1655, 10.1016/j.cmet.2021.05.006 · DOI
  47. Li, 2021, TMEM41B and VMP1 are scramblases and regulate the distribution of cholesterol and phosphatidylserine, J. Cell Biol., 220, e202103105, 10.1083/jcb.202103105 · DOI
  48. Moretti, 2018, TMEM41B is a novel regulator of autophagy and lipid mobilization, EMBO Rep., 19, 10.15252/embr.201845889 · DOI
  49. Hayhurst, 2001, Hepatocyte nuclear factor 4α (nuclear receptor 2A1) is essential for maintenance of hepatic gene expression and lipid homeostasis, Mol. Cell. Biol., 21, 1393, 10.1128/MCB.21.4.1393-1403.2001 · DOI · Paper page
  50. Stoffel, 1997, The maturity-onset diabetes of the young (MODY1) transcription factor HNF4aregulates expression of genes required for glucose transport and metabolism, Proc. Natl. Acad. Sci. USA, 94, 13209, 10.1073/pnas.94.24.13209 · DOI
  51. Tian, 2021, Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis, Nat. Neurosci., 24, 1020, 10.1038/s41593-021-00862-0 · DOI · Paper page
  52. Bersuker, 2019, The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis, Nature, 575, 688, 10.1038/s41586-019-1705-2 · DOI · Paper page
  53. Zhu, 2019, CHP1 regulates compartmentalized glycerolipid synthesis by activating GPAT4, Mol. Cell, 74, 45, 10.1016/j.molcel.2019.01.037 · DOI
  54. Lu, 2022, CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol export, J. Cell Biol., 221, e202105060, 10.1083/jcb.202105060 · DOI
  55. Morris, 2022, A genome-wide CRISPR screen implicates plasma membrane asymmetry in exogenous C6-ceramide toxicity, Biol. Open, 11, bio059695, 10.1242/bio.059695 · DOI
  56. Wang, 2022, Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis, Proc. Natl. Acad. Sci. USA, 119
  57. Piccolis, 2019, Probing the global cellular responses to lipotoxicity caused by saturated fatty acids, Mol. Cell, 74, 32, 10.1016/j.molcel.2019.01.036 · DOI · Paper page
  58. Zhang, 2022, Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination, Mol. Cell, 82, 1528, 10.1016/j.molcel.2022.01.021 · DOI
  59. Stefanovic-Barrett, 2018, March6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins, EMBO Rep., 19, 10.15252/embr.201745603 · DOI
  60. Langmead, 2012, Fast gapped-read alignment with Bowtie 2, Nat. Methods, 9, 357, 10.1038/nmeth.1923 · DOI · Paper page
  61. Schindelin, 2012, Fiji: an open-source platform for biological-image analysis, Nat. Methods, 9, 676, 10.1038/nmeth.2019 · DOI · Paper page
  62. Koelmel, 2017, Expanding lipidome coverage using LC-MS/MS data-dependent acquisition with automated exclusion list generation, J. Am. Soc. Mass Spectrom., 28, 908, 10.1007/s13361-017-1608-0 · DOI · Paper page
  63. Chong, 2019, Using MetaboAnalyst 4.0 for comprehensive and integrative metabolomics data analysis, Curr. Protoc. Bioinformatics, 68, e86, 10.1002/cpbi.86 · DOI
  64. Yang, 2012, Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging, PLoS One, 7, e32693, 10.1371/journal.pone.0032693 · DOI
  65. Mathiowetz, 2023, Protocol for performing pooled CRISPR-Cas9 loss-of-function screens, Star Protoc., 4, 102201, 10.1016/j.xpro.2023.102201 · DOI
  66. Pang, 2022, Using MetaboAnalyst 5.0 for LC-HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data, Nat. Protoc., 17, 1735, 10.1038/s41596-022-00710-w · DOI

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Metadata

Authors
Roberts, M. A., Deol, K. K., Mathiowetz, A. J., Lange, M., Leto, D. E., Stevenson, J., Hashemi, S. H., Morgens, D. W., Easter, E., Heydari, K., Nalls, M. A., Bassik, M. C., Kampmann, M., Kopito, R. R., Faghri, F., & Olzmann, J. A.
ORCID
Olzmann, James A.
Venue
Developmental Cell
Publisher
Elsevier BV
Published
2023-09
Volume
58
Issue
18
Pages
1782-1800.e10
ISSN
1534-5807
DOI
10.1016/j.devcel.2023.07.001
Cited by
81
References
5
Language
en
Licence
Funders
  • U.S. Department of Health and Human Services (ZIAAG000534)
  • Cayman Biomedical Research Institute
  • National Institute on Aging
  • Osaka University
  • Alpha-1 Foundation
  • Universitat de Barcelona
  • Stanford University
  • Rockefeller University
  • National Institutes of Health (F31DK121477, R01DK128099, R01GM074874, R01GM112948, F32GM113370)
  • Chan Zuckerberg Initiative

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