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Cited by 256

The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.

PLGA–PEI nanoparticles for gene delivery to pulmonary epithelium

Open access
Bivas-Benita, Maytal, Romeijn, Stefan, Junginger, Hans E., Borchard, Gerrit·2004·European Journal of Pharmaceutics and Biopharmaceutics
DOI 10.1016/j.ejpb.2004.03.008
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Abstract

Pulmonary gene delivery is thought to play an important role in treating genetically related diseases and may induce immunity towards pathogens entering the body via the airways. In this study we prepared poly (d,l-lactide-co-glycolide) (PLGA) nanoparticles bearing polyethyleneimine (PEI) on their surface and characterized them for their potential in serving as non-viral gene carriers to the pulmonary epithelium. Particles that were synthesized at different PLGA–PEI ratios and loaded with DNA in several PEI–DNA ratios, exhibited narrow size distribution in all formulations, with mean particle sizes ranging between 207 and 231 nm. Zeta potential was strongly positive (above 30 mV) for all the PEI–DNA ratios examined and the loading efficiency exceeded 99% for all formulations. Internalization of the DNA-loaded PLGA–PEI nanoparticles was studied in the human airway submucosal epithelial cell line, Calu-3, and DNA was detected in the endo-lysosomal compartment 6 h after particles were applied. Cytotoxicity of these nanoparticles was dependent on the PEI–DNA ratio and best cell viability was achieved by PEI–DNA ratios 1:1 and 0.5:1. These findings demonstrate that PLGA–PEI nanoparticles are a potential new delivery system to carry genes to the lung epithelium.

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Metadata

Authors
Bivas-Benita, M., Romeijn, S., Junginger, H. E., & Borchard, G.
Venue
European Journal of Pharmaceutics and Biopharmaceutics
Publisher
Elsevier BV
Published
2004-07
Volume
58
Issue
1
Pages
1-6
ISSN
0939-6411 (ISSN-L)
DOI
10.1016/j.ejpb.2004.03.008
OpenAlex
W2086886701
Cited by
256
References
28
FWCI
3.50
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: PLGA–PEI nanoparticles for gene delivery to pulmonary epithelium. https://app.cochat.ai/papers/10.1016/j.ejpb.2004.03.008
The paper
Bivas-Benita, M., Romeijn, S., Junginger, H. E., & Borchard, G. (2004). PLGA–PEI nanoparticles for gene delivery to pulmonary epithelium. European Journal of Pharmaceutics and Biopharmaceutics. https://doi.org/10.1016/j.ejpb.2004.03.008

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.