Cited by 178Viral Infectious Diseases and Gene Expression in InsectsMonoclonal and Polyclonal Antibodies ResearchProtein purification and stability

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Guidelines to cell engineering for monoclonal antibody production

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Rita Costa, A., Elisa Rodrigues, M., Henriques, Mariana, Azeredo, Joana, Oliveira, Rosário·2010·European Journal of Pharmaceutics and Biopharmaceutics

DOI 10.1016/j.ejpb.2009.10.002

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Abstract

Monoclonal antibodies (mAbs) are currently used for many diagnostic and therapeutic applications. The high demand for these biopharmaceuticals has led to the development of large-scale manufacturing processes, with productivity improvements being mainly achieved by optimization of bioreactor systems. However, more recently, the early steps of production, previous to bioreactor culture, have been presented as alternative areas where productivity enhancements can be achieved. Thus, this review describes the progress made for the improvement of productivity in mammalian expression systems for the high production of mAbs. Advances in the development of mAb-producing cell lines are being made, particularly regarding expression vector design and methods used for transfection, with the intent to create a reproducible methodology. Selection of the most suitable clones is also a critical step that can be improved, by including variables other than the expression level, which is still the common practice. Furthermore, strategies of cell engineering, although still mostly based on trial-and-error experimentation and not in standard protocols, hold great interest to improve cell growth and productivity, as well as product quality in the future. Improvements of the initial steps of the production process would not only result in cells with higher expression ability, but would also speed-up the process development.

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Trends in Upstream and Downstream Process Development for Antibody Manufacturing
Gronemeyer, P., Ditz, R., & Strube, J. (2014) · Bioengineering · doi:10.3390/bioengineering1040188 · Cited by 347
Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296
Perfusion mammalian cell culture for recombinant protein manufacturing – A critical review
Bielser, J., Wolf, M., Souquet, J., Broly, H., & Morbidelli, M. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.04.011 · Cited by 284
Advances in the production and downstream processing of antibodies
Chon, J. H., & Zarbis‐Papastoitsis, G. (2011) · New Biotechnology · doi:10.1016/j.nbt.2011.03.015 · Cited by 230
Gene Expression in Mammalian Cells and its Applications
Khan, K. H. (2013) · Europe PMC (PubMed Central) · doi:10.5681/apb.2013.042 · Cited by 166

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Authors: Rita Costa, A., Elisa Rodrigues, M., Henriques, M., Azeredo, J., & Oliveira, R.
Affiliations: Institute for Biotechnology and Bioengineering (PT)
Corresponding author: Henriques, Mariana
ORCID: Henriques, Mariana
Azeredo, Joana
Venue: European Journal of Pharmaceutics and Biopharmaceutics
Publisher: Elsevier BV
Published: 2010-02
Volume: 74
Issue: 2
Pages: 127-138
ISSN: 0939-6411 (ISSN-L)
DOI: 10.1016/j.ejpb.2009.10.002
OpenAlex: W2108422551
Cited by: 178
References: 220
Citations by year: 2026: 2 · 2025: 8 · 2024: 10 · 2023: 12 · 2022: 8 · 2021: 14 · 2020: 11 · 2019: 9 · 2018: 13 · 2017: 17 · 2016: 8 · 2015: 10 · 2014: 15 · 2013: 20 · 2012: 10
FWCI: 2.80
Type: journal article
Language: en
Open access: green
hdl.handle.net
Licence: other-oa
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: European Journal of Pharmaceutics and Biopharmaceutics (publishedVersion)
PubMed (publishedVersion)
RepositóriUM (Universidade do Minho) (submittedVersion, other-oa)
Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
Keywords: monoclonal antibodies, cell engineering, mammalian cell expression systems, expression vector design, transfection, clone selection, antibody productivity, biopharmaceutical manufacturing, cell growth
MeSH terms: Animals, Antibodies, Monoclonal, Biotechnology, Humans, Technology, Pharmaceutical, Transfection, Protein Engineering, Cell Culture Techniques

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Guidelines to cell engineering for monoclonal antibody production

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