Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells

Mohan

doi:10.1002/biot.200700249

Cited by 102Viral Infectious Diseases and Gene Expression in InsectsProtein purification and stabilityTransgenic Plants and Applications

Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells

Mohan·2008·Biotechnology Journal

DOI 10.1002/biot.200700249

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Abstract

Recombinant glycoprotein therapeutics have proven to be invaluable pharmaceuticals for the treatment of various diseases. Chinese hamster ovary (CHO) cells are widely used in industry for the production of these proteins. Several strategies for engineering CHO cells for improved protein production have been tried with considerable results. The focus has mainly been to increase the specific productivity and to extend the culture longevity by preventing programmed cell death. These CHO cell engineering strategies, particularly those developed in Korea, are reviewed here.

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Chaperones have been employed by many groups as cell engineering tools but have given variable results.
“They have been employed by many groups as cell engineering tools but have given variable results (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
The BiP chaperone gave mixed results, with enhanced or decreased productivity.
“Similarly, the heavy chain-binding protein (BiP) chaperone gave mixed results, with enhanced or decreased productivity (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
Overexpression of ERp57 together with calnexin/calreticulin was able to increase thrombopoietin production in CHO cells.
“Moreover, overexpression of the endoplasmic-reticulum ERp57 protein, an isoform of PDI, together with calnexin/calreticulin was able to increase thrombopoietin production in CHO cells (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
Other studies observed either a negative or no effect on productivity with PDI overexpression.
“However, other studies observed either a negative or no effect on productivity (Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008
Translational and/or post-translational processes might also be rate-limiting for protein production, because of the lack of correlation between gene copy number and the amount of secreted proteins.
“It has been suggested that translational and/or post-translational processes might also be rate-limiting for protein production, because of the lack of correlation between gene copy number and the amount of secreted proteins (Wilson et al., 1990;Mohan et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Mohan et al., 2008

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Authors: Mohan
Venue: Biotechnology Journal
Publisher: Wiley
Published: 2008-05
Volume: 3
Issue: 5
Pages: 624-630
ISSN: 1860-6768 (ISSN-L)
1860-7314
DOI: 10.1002/biot.200700249
OpenAlex: W2077304355
Cited by: 102
References: 41
Citations by year: 2025: 2 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 8 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 4 · 2013: 12 · 2012: 9
FWCI: 0.67
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Biotechnology Journal (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
Keywords: CHO cell engineering, therapeutic proteins, specific productivity, culture longevity, programmed cell death, CHO cells, bioprocess optimization
MeSH terms: Animals, Cricetulus, Cricetinae, Recombinant Proteins, Technology Assessment, Biomedical, Protein Engineering, CHO Cells, Genetic Enhancement

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Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells

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