Anti-Oxidative and Anti-Aging Activities of 2-O-α-Glucopyranosyl-L-Ascorbic Acid on Human Dermal Fibroblasts
Abstract
A stable ascorbic acid derivative, 2-O-α-glucopyranosyl-l-ascorbic acid (AA-2G), was evaluated and compared with ascorbic acid for its protective effect against cellular damage and senescence induced by hydrogen peroxide (H(2)O(2)). Pretreatment with AA-2G for 72 h promoted the proliferation of normal human dermal fibroblasts (NHDF) and protected against cell damage induced by H(2)O(2). In contrast, ascorbic acid increased the proliferation and protected against cell damage, only when culture medium containing ascorbic acid was replaced every 24 h during the pretreatment period. These results suggest that the effect of AA-2G is longer-lasting compared to that of ascorbic acid. Senescence associated-β-galactosidase (SA-β-gal) activity, a classical biomarker of cellular senescence, was increased in H(2)O(2)-exposed NHDF cells, but pretreatment or posttreatment with ascorbic acid or AA-2G significantly inhibited the increase in SA-β-gal levels. AA-2G was more potent than ascorbic acid in down-regulating SA-β-gal activity. Expression of SIRT1, which has attracted attention as an anti-aging factor in recent years, was significantly decreased in H(2)O(2)-exposed NHDF cells compared to untreated cells. However, pretreatment NHDF cells with AA-2G before H(2)O(2) exposure significantly inhibited this decrease in SIRT1 expression, whereas ascorbic acid had no effect. After H(2)O(2) exposure, the expression levels of p53 and p21 were increased in NHDF cells and pretreatment with AA-2G inhibited this increase. Together, these results suggest that AA-2G protects dermal fibroblasts from oxidative stress and cellular senescence. These characteristics indicate that AA-2G could become a promising material for its anti-aging properties.
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The effect of AA-2G is longer-lasting compared to that of AA against cellular damage and senescence induced by hydrogen peroxide.
“The results suggest that the effect of AA-2G is longer-lasting compared to that of AA and this derivative might therefore be considered as a more stable form of vitamin C [130].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [130]
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The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence
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- Authors
- Taniguchi, M., Arai, N., Kohno, K., Ushio, S., & Fukuda, S.
- Affiliations
- Hayashibara (Japan) (JP)
- Corresponding author
- Taniguchi, Mutsuko
- ORCID
- Kohno, Keizo
- Venue
- European Journal of Pharmacology
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 674
- Issue
- 2-3
- Pages
- 126-131
- ISSN
- 0014-2999 (ISSN-L)
- OpenAlex
- W2044836520
- Cited by
- 62
- References
- 38
- Citations by year
- 2026: 1 · 2024: 6 · 2023: 8 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 2 · 2017: 10 · 2016: 5 · 2015: 3 · 2014: 6 · 2013: 2 · 2012: 3
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Keywords
- ascorbic acid derivatives, oxidative stress, cellular senescence, dermal fibroblasts, hydrogen peroxide, SIRT1, p53, p21, SA-β-galactosidase, anti-aging, antioxidant activity, cell proliferation
- MeSH terms
- Antioxidants, Ascorbic Acid, Cell Cycle, DNA Repair, Fibroblasts, Gene Expression Regulation, Humans, Hydrogen Peroxide, Skin, Transcription, Genetic, Biomarkers, Tumor Suppressor Protein p53, Cellular Senescence, Cyclin-Dependent Kinase Inhibitor p21, Sirtuin 1
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