Ascorbic acid extends replicative life span of human embryonic fibroblast by reducing DNA and mitochondrial damages
Open accessAbstract
Ascorbic acid has been reported to extend replicative life span of human embryonic fibroblast (HEF). Since the detailed molecular mechanism of this phenomenon has not been investigated, we attempted to elucidate. Continuous treatment of HEF cells with ascorbic acid (at 200 microM) from 40 population doubling (PD) increased maximum PD numbers by 18% and lowered SA-beta-gal positive staining, an aging marker, by 2.3 folds, indicating that ascorbic acid extends replicative life span of HEF cells. Ascorbic acid treatment lowered DCFH by about 7 folds and Rho123 by about 70%, suggesting that ascorbic acid dramatically decreased ROS formation. Ascorbic acid also increased aconitase activity, a marker of mitochondrial aging, by 41%, indicating that ascorbic acid treatment restores age-related decline of mitochondrial function. Cell cycle analysis by flow cytometry revealed that ascorbic acid treatment decreased G1 population up to 12%. Further western blot analysis showed that ascorbic acid treatment decreased levels of p53, phospho-p53 at ser 15, and p21, indicating that ascorbic acid relieved senescence-related G1 arrest. Analysis of AP (apurinic/apyrimidinic) sites showed that ascorbic acid treatment decreased AP site formation by 35%. We also tested the effect of hydrogen peroxide treatment, as an additional oxidative stress. Continuous treatment of 20 microM of hydrogen peroxide from PD 40 of HEF cells resulted in premature senescence due to increased ROS level, and increased AP sites. Taken together, the results suggest that ascorbic acid extends replicative life span of HEF cells by reducing mitochondrial and DNA damages through lowering cellular ROS.
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Ascorbic acid extends replicative lifespan of human embryonic fibroblasts by restoring age-related decline of mitochondrial function and lowering cellular ROS, therefore reducing mitochondrial and DNA damages with decelerated telomere shortening.
“For example, AA has been reported to extend replicative lifespan of human embryonic fibroblasts by restoring age-related decline of mitochondrial function and lowering cellular ROS, therefore reducing mitochondrial and DNA damages with decelerated telomere shortening [123,124].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [123]
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- Authors
- Hwang, W.-S., Park, S.-H., Kim, H.-S., Kang, H.-J., Kim, M.-J., Oh, S.-J., Park, J.-B., Kim, J., Kim, S. C., & Lee, J.-Y.
- Affiliations
- Hallym University (KR)
- Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Gangwon 200-702, Korea.
- Corresponding author
- Lee, Jae-Yong
- Venue
- Nutrition Research and Practice
- Publisher
- XMLink
- Published
- 2007
- Volume
- 1
- Issue
- 2
- Pages
- 105
- ISSN
- 1976-1457 (ISSN-L)
- PMC
- PMC2882584
- OpenAlex
- W2163530436
- Cited by
- 21
- References
- 35
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2020: 3 · 2018: 1 · 2016: 1 · 2015: 4 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nutrition Research and Practice (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Hallym University
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- vitamin C, replicative lifespan, cellular senescence, human embryonic fibroblasts, reactive oxygen species, oxidative stress, DNA damage, abasic sites, mitochondrial damage, aconitase activity, p53, p21, G1 cell cycle arrest, hydrogen peroxide, SA-β-galactosidase
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