Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes
Open accessAbstract
Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in the cells, which induces the cellular senescence and photoaging. The present study investigated the protective effects of garlic on photo-damage and cellular senescence in UVB-exposed human keratinocytes, HaCaT cells. An in vitro cell free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and nitric oxide (NO). The effect of garlic extract on ROS formation, MMP-1 protein and mRNA expressions, cytokines such as interleukin (IL)-1β and IL-6, senescence associated-β-galactosidase (SA-β-gal) activity, and silent information regulator T1 (SIRT1) activity were determined in UVB-irradiated HaCaT cells. Garlic exhibited strong DPPH radical and NO scavenging activity in cell free system exhibiting IC50 values of 2.50 mg/mL and 4.38 mg/mL, respectively. Garlic pretreatment attenuated the production of UVB-induced intracellular ROS. MMP-1 level, which has been known to be induced by ROS, was dramatically elevated by UVB irradiation, and UVB-induced MMP-1 mRNA and protein expressions were significantly reduced by garlic treatment (50 µg/mL) comparable to those of UV-unexposed control cells. UV-induced pro-inflammatory cytokine productions (IL-6 and IL-1β) were significantly inhibited by pretreatment with garlic in a dose-dependent manner. SA-β-gal activity, a classical biomarker of cellular senescence, and SIRT1 activity, which has attracted attention as an anti-aging factor in recent years, were ameliorated by garlic treatment in UV-irradiated HaCaT cells. The present study provides the first evidence of garlic inhibiting UVB-induced photoaging as a result of augmentation of cellular senescence in HaCaT human keratinocytes.
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When used as a whole, garlic extract exerts strong NO scavenging function, reduces MMP-1 expression and ROS levels, inhibits SASP and improves SIRT1 activity, thus alleviating UVB-induced senescence in keratinocytes.
“Still, when used as a whole, garlic extract exerts a strong NO scavenging function, reduces MMP-1 expression and ROS levels, inhibits SASP and improves SIRT1 activity, thus alleviating UVB-induced senescence in keratinocytes [212].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [212]
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- Authors
- Kim, H.
- Affiliations
- Hanseo University (KR)
- Corresponding author
- Kim, Hye
- ORCID
- Kim, Hye
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2016-07-29
- Volume
- 8
- Issue
- 8
- Pages
- 464
- ISSN
- 2072-6643 (ISSN-L)
- PMC
- PMC4997377
- OpenAlex
- W2493916098
- Cited by
- 67
- References
- 48
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 4 · 2023: 4 · 2022: 14 · 2021: 10 · 2020: 8 · 2019: 12 · 2018: 5 · 2016: 1
- FWCI
- 7.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nutrients (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- 2015 Fundamental R&D Program of Hanseo University
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- garlic, cellular senescence, UVB irradiation, photoaging, HaCaT keratinocytes, reactive oxygen species, MMP-1, inflammatory cytokines, IL-1β, interleukin-6, senescence-associated β-galactosidase, SIRT1, antioxidant activity, nitric oxide scavenging, DPPH radical scavenging
- MeSH terms
- beta-Galactosidase, Cell Line, Transformed, Garlic, Gene Expression Regulation, Humans, Plant Extracts, Radiation-Protective Agents, RNA, Messenger, Ultraviolet Rays, Biomarkers, Skin Aging, Keratinocytes, Free Radical Scavengers, Cytokines, Cellular Senescence, Reactive Oxygen Species, Plant Roots, Dietary Supplements, Matrix Metalloproteinase 1, Sirtuin 1
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