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Cited by 36Folate and B Vitamins ResearchCardiovascular Function and Risk FactorsBiochemical effects in animals

Heart dysfunction induced by choline-deficiency in adult rats: The protective role of l-carnitine

Strilakou, Athina A., Lazaris, Andreas C., Perelas, Apostolos I., Mourouzis, Iordanis S., Douzis, Ioannis Ch., Karkalousos, Petros L., Stylianaki, Aikaterini Th., Pantos, Costas I. +1 more·2013·European Journal of Pharmacology
DOI 10.1016/j.ejphar.2013.03.025
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Abstract

Choline is a B vitamin co-factor and its deficiency seems to impair heart function. Carnitine, a chemical analog of choline, has been used as adjunct in the management of cardiac diseases. The study investigates the effects of choline deficiency on myocardial performance in adult rats and the possible modifications after carnitine administration. Wistar Albino rats (n=24), about 3 months old, were randomized into four groups fed with: (a) standard diet (control-CA), (b) choline deficient diet (CDD), (c) standard diet and carnitine in drinking water 0.15% w/v (CARN) and (d) choline deficient diet and carnitine (CDD+CARN). After four weeks of treatment, we assessed cardiac function under isometric conditions using the Langendorff preparations [Left Ventricular Developed Pressure (LVDP-mmHg), positive and negative first derivative of LVDP were evaluated], measured serum homocysteine and brain natriuretic peptide (BNP) levels and performed histopathology analyses. In the CDD group a compromised myocardium contractility compared to control (P=0.01), as assessed by LVDP, was noted along with a significantly impaired diastolic left ventricular function, as assessed by (-) dp/dt (P=0.02) that were prevented by carnitine. Systolic force, assessed by (+) dp/dt, showed no statistical difference between groups. A significant increase in serum BNP concentration was found in the CDD group (P<0.004) which was attenuated by carnitine (P<0.05), whereas homocysteine presented contradictory results (higher in the CDD+CARN group). Heart histopathology revealed a lymphocytic infiltration of myocardium and valves in the CDD group that was reduced by carnitine. In conclusion, choline deficiency in adult rats impairs heart performance; carnitine acts against these changes.

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Authors
Strilakou, A. A., Lazaris, A. C., Perelas, A. I., Mourouzis, I. S., Douzis, I. C., Karkalousos, P. L., Stylianaki, A. T., Pantos, C. I., & Liapi, C. A.
Affiliations
  • National and Kapodistrian University of Athens (GR)
  • Technological Educational Institute of Athens (GR)
Corresponding author
Liapi, Charis A.
ORCID
Venue
European Journal of Pharmacology
Publisher
Elsevier BV
Published
2013-06
Volume
709
Issue
1-3
Pages
20-27
ISSN
0014-2999 (ISSN-L)
DOI
10.1016/j.ejphar.2013.03.025
OpenAlex
W2023023873
Cited by
36
References
69
Citations by year
2026: 1 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 8 · 2019: 3 · 2018: 3 · 2017: 3 · 2015: 8 · 2013: 1
FWCI
2.45
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • European Journal of Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rheumatology › Folate and B Vitamins Research
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
  • Health Sciences › Medicine › Physiology › Biochemical effects in animals
Keywords
choline deficiency, L-carnitine, cardiac dysfunction, cardiac contractility, diastolic dysfunction, brain natriuretic peptide, homocysteine, choline-deficient diet, cardioprotection
MeSH terms
Animals, Cardiotonic Agents, Carnitine, Choline Deficiency, Edema, Cardiac, Fibrosis, Heart Valves, Heart Ventricles, Homocysteine, Lymphocytes, Male, Myocardial Contraction, Random Allocation, Rats, Wistar, Ventricular Dysfunction, Left, Dietary Supplements, Natriuretic Peptide, Brain, Hyperhomocysteinemia, Rats

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