Cited by 16Fatty Acid Research and HealthInflammatory mediators and NSAID effectsEdible Oils Quality and Analysis

Eicosapentaenoic acid monoglyceride resolves inflammation in an ex vivo model of human peripheral blood mononuclear cell

Morin, Caroline, Charbonneau, Léane, Ouellet, Nicole, Ouellet, Hélène, Blier, Pierre U., Dufresne, France, Fortin, Samuel·2017·European Journal of Pharmacology

Abstract

Phosphorylation and activation of p38 MAPK and NFκB pathways, along with the resulting overproduction of interleukin IL-1β, IL-6, and tumor necrosis factor a (TNFα) is a hallmark of inflammatory disorders. Omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementations are known to exert anti-inflammatory properties by reduction of keys cytokines and enzymes involved in inflammation. Here, we investigated the anti-inflammatory pathways and mediators modulated by eicosapentaenoic acid monoglyceride (MAG-EPA) on human peripheral blood mononuclear cells (PBMCs) from healthy donors and stimulated, ex vivo, with lipopolysaccharide (LPS). LPS stimulation increased p38 MAPK and NFκB phosphorylation, which was abolished by MAG-EPA treatments. Concomitantly, MAG-EPA also abolished LPS-induced inflammation in PBMCs by reducing IL-1β, IL-6, and TNFα cytokines at protein and transcript levels. Moreover, MAG-EPA decreased the levels of HIF1α in LPS-induced human PBMCs. Results also revealed a decreased of pro-inflammatory enzymes such as Cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) in LPS-induced PBMCs. Altogether, the present data suggest that MAG-EPA, represents a new potential therapeutic strategy for resolving inflammation in inflammatory disorders including autoimmune diseases, allergies, asthma, arthritis and cancer.

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Crossref · 10.1016/j.ejphar.2017.05.018

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Authors
Morin, C., Charbonneau, L., Ouellet, N., Ouellet, H., Blier, P. U., Dufresne, F., & Fortin, S.
Affiliations
Université du Québec à Rimouski (CA)
Corresponding author
Fortin, Samuel
Venue
European Journal of Pharmacology
Publisher
Elsevier BV
Published
2017-07
Volume
807
Pages
205-211
ISSN
0014-2999 (ISSN-L)
OpenAlex
W2613043864
Cited by
16
References
26
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 1
FWCI
2.73
Type
journal article
Language
en
Open access
closed
Locations
  • European Journal of Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Keywords
omega-3 polyunsaturated fatty acids, anti-inflammatory activity, peripheral blood mononuclear cells, lipopolysaccharide-induced inflammation, p38 MAPK, NF-κB signaling, inflammatory cytokines, IL-1β, interleukin-6, TNF-α, HIF-1α, cyclooxygenase-2, 5-lipoxygenase
MeSH terms
Adult, Arachidonate 15-Lipoxygenase, Arachidonate 5-Lipoxygenase, Enzyme Activation, Humans, Inflammation, Leukocytes, Mononuclear, Mitogens, RNA, Messenger, Biomarkers, Gene Expression Regulation, Enzymologic, Cytokines, MAP Kinase Signaling System, Cell Proliferation, Monoglycerides, Hypoxia-Inducible Factor 1, alpha Subunit, Young Adult

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