Cited by 94Toxic Organic Pollutants ImpactEpigenetics and DNA MethylationHealth, Environment, Cognitive Aging

Global DNA hypermethylation is associated with high serum levels of persistent organic pollutants in an elderly population

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Lind, Lars, Penell, Johanna, Luttropp, Karin, Nordfors, Louise, Syvänen, Anne-Christine, Axelsson, Tomas, Salihovic, Samira, van Bavel, Bert +3 more·2013·Environment International

Abstract

Dioxin exposure has experimentally been associated with changes in DNA methylation, an epigenetic change that is associated with disease. The present study aims to investigate if serum levels of dioxin and other persistent environmental pollutants are related to global DNA methylation in a human sample. In the population-based Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study (all aged 70), global DNA methylation was measured by the Luminometric Methylation Assay in 524 subjects. Twenty-three different POPs, including 16 PCBs, five pesticides, one dioxin (OCDD) and one brominated flame retardant (BDE47) were analysed by HRGC/HRMS. Ten single nucleotide polymorphisms (SNPs) in the Aryl hydrocarbon (Ah)-receptor were analysed by mini-sequencing. High levels of toxic equivalency (TEQ) for PCBs and dioxin were associated with DNA hypermethylation (p=0.030). This was mainly attributed to coplanar non-ortho PCBs. While no significant associations were found between DNA methylation and SNPs in the Ah-receptor, an interaction was found between the SNP rs2237297 and TEQ so that TEQ was associated with hypermethylation (p=0.009) only in subjects with one G-allele (n=103). Also high levels of the PCB126 congener, the OCDD, and the pesticide metabolite p,p'-DDE were related to DNA hypermethylation (p=0.01, 0.03 and 0.003, respectively). In conclusion, in a sample of elderly subjects, high TEQ including PCBs and the dioxin OCDD and high serum levels of PCB126, OCDD, and p,p'-DDE were related to global DNA hypermethylation in a cross-sectional analysis.

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Crossref · 10.1016/j.envint.2013.07.008

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  • DL and NDL PCBs have opposing actions on DNA methylation, with DL PCBs shifting the balance toward DNA hypermethylation and NDL PCBs favoring DNA hypomethylation.

    “There is evidence to suggest that the DL and NDL PCBs have opposing actions on DNA methylation, with DL PCBs shifting the balance toward DNA hypermethylation, as was observed in the Sweden study [ 39 ], and NDL PCBs favoring DNA hypomethylation as observed in the Korean and Inuit studies [ 36 , 38 ].”
  • In a separate study of 524 elderly men and women (70 years of age) living in Uppsala, Sweden, high levels of DL PCBs were associated with global DNA hypermethylation.

    “Conversely, in a separate study of 524 elderly men and women (70 years of age) living in Uppsala, Sweden, high levels of DL PCBs were associated with global DNA hypermethylation [ 39 ].”

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Authors
Lind, L., Penell, J., Luttropp, K., Nordfors, L., Syvänen, A.-C., Axelsson, T., Salihovic, S., van Bavel, B., Fall, T., Ingelsson, E., & Lind, P. M.
Affiliations
  • Uppsala University (SE)
  • Karolinska Institutet (SE)
Corresponding author
Lind, P. Monica
Venue
Environment International
Publisher
Elsevier BV
Published
2013-09
Volume
59
Pages
456-461
ISSN
0160-4120 (ISSN-L)
OpenAlex
W2077502047
Cited by
94
References
33
Citations by year
2026: 2 · 2025: 4 · 2024: 7 · 2023: 6 · 2022: 10 · 2021: 7 · 2020: 8 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 15 · 2015: 5 · 2014: 4
FWCI
5.86
Type
journal article
Language
en
Open access
Locations
  • Environment International (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Open Research (University of Surrey) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Surrey Research Insight Open Access (The University of Surrey) (submittedVersion)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Health, Environment, Cognitive Aging
Keywords
persistent organic pollutants, dioxins, polychlorinated biphenyls, PCB 126, p,p'-DDE, brominated flame retardants, pesticides, toxic equivalents, aryl hydrocarbon receptor, single nucleotide polymorphisms, epigenetics, older adults, PIVUS study
MeSH terms
Polychlorinated Dibenzodioxins, Aged, Cross-Sectional Studies, Dichlorodiphenyl Dichloroethylene, Dioxins, Environmental Pollutants, Female, Humans, Male, Pesticides, Polychlorinated Biphenyls, Prospective Studies, Receptors, Aryl Hydrocarbon, DNA Methylation, Halogenated Diphenyl Ethers

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