Cited by 189Nanoparticles: synthesis and applicationsGold and Silver Nanoparticles Synthesis and ApplicationsLaser-Ablation Synthesis of Nanoparticles

Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications

Singh, Priyanka, Singh, Hina, Kim, Yeon Ju, Mathiyalagan, Ramya, Wang, Chao, Yang, Deok Chun·2016·Enzyme and Microbial Technology

Abstract

The present study highlights the microbial synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 strain, in an efficient way. The synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. The spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. The energy dispersive X-ray spectroscopy and elemental mapping were displayed the purity and maximum elemental distribution of silver and gold elements in the respective nanoproducts. The X-ray diffraction spectroscopy results demonstrate the crystalline nature of synthesized nanoparticles. The particle size analysis demonstrate the nanoparticles distribution with respect to intensity, volume and number of nanoparticles. For biological applications, the silver nanoparticles have been explored in terms of MIC and MBC against pathogenic microorganisms such as Vibrio parahaemolyticus, Escherichia coli, Salmonella enterica, Bacillus anthracis, Bacillus cereus and Staphylococcus aureus. Moreover, the silver nanoparticles in combination with commercial antibiotics, such as vancomycin, rifampicin, oleandomycin, penicillin G, novobiocin, and lincomycin have been explored for the enhancement of antibacterial activity and the obtained results showed that 3μg concentration of silver nanoparticles sufficiently enhance the antimicrobial efficacy of commercial antibiotics against pathogenic microorganism. Furthermore, the silver nanoparticles potential has been reconnoitered for the biofilm inhibition by S. aureus, Pseudomonas aeruginosa and E. coli and the results revealed sufficient activity at 6μg concentration. In addition, gold nanoparticles have been applied for catalytic activity, for the reduction of 4-nitrophenol to 4-aminophenol using sodium borohydride and positive results were attained.

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Crossref · 10.1016/j.enzmictec.2016.02.005

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  • Gold nanoparticles are appealing for targeted drug delivery, photothermal therapy (PTT), photodynamic therapy (PDT), photoimaging, biosensors, and photothermal therapy.

    “They are appealing for targeted drug delivery, photothermal therapy (PTT), photodynamic therapy (PDT), photoimaging, biosensors, and photothermal therapy.84”

    From Smart nanoparticles for cancer therapy (2023) · cites this paper as [84]

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Authors
Singh, P., Singh, H., Kim, Y. J., Mathiyalagan, R., Wang, C., & Yang, D. C.
Affiliations
Kyung Hee University (KR)
Corresponding author
  • Kim, Yeon Ju
  • Yang, Deok Chun
Venue
Enzyme and Microbial Technology
Publisher
Elsevier BV
Published
2016-05
Volume
86
Pages
75-83
ISSN
0141-0229 (ISSN-L)
OpenAlex
W2284055764
Cited by
189
References
40
Citations by year
2026: 10 · 2025: 11 · 2024: 18 · 2023: 13 · 2022: 28 · 2021: 30 · 2020: 15 · 2019: 21 · 2018: 13 · 2017: 18 · 2016: 12
FWCI
9.49
Type
journal article
Language
en
Open access
closed
Locations
  • Enzyme and Microbial Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, and Forestry & Fisheries (313038-03-2-SB020)
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Laser-Ablation Synthesis of Nanoparticles
Keywords
silver nanoparticles, gold nanoparticles, green synthesis, extracellular synthesis, antibacterial activity, antibiotic enhancement, biofilm inhibition, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, 4-nitrophenol reduction, catalytic reduction, vancomycin, rifampicin, lincomycin
MeSH terms
Anti-Infective Agents, Gold, Microbial Sensitivity Tests, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Silver, Nanotechnology, Metal Nanoparticles, Sporosarcina

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