Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications
Abstract
The present study highlights the microbial synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 strain, in an efficient way. The synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. The spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. The energy dispersive X-ray spectroscopy and elemental mapping were displayed the purity and maximum elemental distribution of silver and gold elements in the respective nanoproducts. The X-ray diffraction spectroscopy results demonstrate the crystalline nature of synthesized nanoparticles. The particle size analysis demonstrate the nanoparticles distribution with respect to intensity, volume and number of nanoparticles. For biological applications, the silver nanoparticles have been explored in terms of MIC and MBC against pathogenic microorganisms such as Vibrio parahaemolyticus, Escherichia coli, Salmonella enterica, Bacillus anthracis, Bacillus cereus and Staphylococcus aureus. Moreover, the silver nanoparticles in combination with commercial antibiotics, such as vancomycin, rifampicin, oleandomycin, penicillin G, novobiocin, and lincomycin have been explored for the enhancement of antibacterial activity and the obtained results showed that 3μg concentration of silver nanoparticles sufficiently enhance the antimicrobial efficacy of commercial antibiotics against pathogenic microorganism. Furthermore, the silver nanoparticles potential has been reconnoitered for the biofilm inhibition by S. aureus, Pseudomonas aeruginosa and E. coli and the results revealed sufficient activity at 6μg concentration. In addition, gold nanoparticles have been applied for catalytic activity, for the reduction of 4-nitrophenol to 4-aminophenol using sodium borohydride and positive results were attained.
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Gold nanoparticles are appealing for targeted drug delivery, photothermal therapy (PTT), photodynamic therapy (PDT), photoimaging, biosensors, and photothermal therapy.
“They are appealing for targeted drug delivery, photothermal therapy (PTT), photodynamic therapy (PDT), photoimaging, biosensors, and photothermal therapy.84”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [84]
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Self-citation
- Authors
- Singh, P., Singh, H., Kim, Y. J., Mathiyalagan, R., Wang, C., & Yang, D. C.
- Affiliations
- Kyung Hee University (KR)
- Corresponding author
- Kim, Yeon Ju
- Yang, Deok Chun
- Venue
- Enzyme and Microbial Technology
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 86
- Pages
- 75-83
- ISSN
- 0141-0229 (ISSN-L)
- OpenAlex
- W2284055764
- Cited by
- 189
- References
- 40
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 18 · 2023: 13 · 2022: 28 · 2021: 30 · 2020: 15 · 2019: 21 · 2018: 13 · 2017: 18 · 2016: 12
- FWCI
- 9.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Enzyme and Microbial Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korea Institute of Planning & Evaluation for Technology in Food, Agriculture, and Forestry & Fisheries (313038-03-2-SB020)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Laser-Ablation Synthesis of Nanoparticles
- Keywords
- silver nanoparticles, gold nanoparticles, green synthesis, extracellular synthesis, antibacterial activity, antibiotic enhancement, biofilm inhibition, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, 4-nitrophenol reduction, catalytic reduction, vancomycin, rifampicin, lincomycin
- MeSH terms
- Anti-Infective Agents, Gold, Microbial Sensitivity Tests, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Silver, Nanotechnology, Metal Nanoparticles, Sporosarcina
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