Cited by 102Nutrition and Health in AgingSirtuins and Resveratrol in MedicineMuscle Physiology and Disorders

The effect of exercise, resveratrol or their combination on Sarcopenia in aged rats via regulation of AMPK/Sirt1 pathway

Liao, Zhi-Yin, Chen, Jin-Liang, Xiao, Ming-Han, Sun, Yue, Zhao, Yu-Xing, Pu, Die, Lv, An-Kang, Wang, Mei-Li +4 more·2017·Experimental Gerontology

Abstract

Sarcopenia is an age-related syndrome characterized by progressive loss of muscle mass and function. Exercise is an important strategy to prolong life and increase muscle mass, and resveratrol has been shown a variety beneficial effects on skeletal muscle. In the present study, we investigated the potential efficacy of using short-term exercise (six weeks), resveratrol (150mg/kg/day), or combined exercise+resveratrol (150mg/kg/day) on gastrocnemius muscle mass, grip strength, cross-sectional area and microscopic morphology in aged rats, and explored the potential mechanism at the apoptosis level. Six months old SD rats were used as young control group and 24months old SD rats were adopted as aged group. After six weeks intervention, the data provide evidence that exercise, resveratrol or their combination significantly increase the relative grip strength and muscle mass in aged rats (P<0.05). Electron microscopy discovered a significant increase in sarcomere length, I-band and H-zone in aged rats (P<0.05), and exercise, resveratrol or their combination significantly reduced the increasement (P<0.05). Moreover, light microscopy revealed a significant increase on Feret's diameter and cross-sectional area (CSA) in aged rats (P<0.05), but exercise and resveratrol did not show significant effects on them (P>0.05). Furthermore, exercise, resveratrol or their combination significantly increased the expression of p-AMPK and SIRT1, decreased the expression of acetyl P53 and Bax/Bcl-2 ratio in aged rats (P<0.05). These findings show that aged rats show significant changes in gastrocnemius muscle morphology and ultrastructure, and the protective effects of exercise, resveratrol and their combination are probably associated with anti-apoptotic signaling pathways through activation of AMPK/Sirt1.

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Crossref · 10.1016/j.exger.2017.08.032

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Authors
Liao, Z.-Y., Chen, J.-L., Xiao, M.-H., Sun, Y., Zhao, Y.-X., Pu, D., Lv, A.-K., Wang, M.-L., Zhou, J., Zhu, S.-Y., Zhao, K.-X., & Xiao, Q.
Affiliations
The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
Corresponding author
Xiao, Qian
Venue
Experimental Gerontology
Publisher
Elsevier BV
Published
2017-11
Volume
98
Pages
177-183
ISSN
0531-5565 (ISSN-L)
OpenAlex
W2746104974
Cited by
102
References
49
Citations by year
2026: 6 · 2025: 14 · 2024: 12 · 2023: 12 · 2022: 9 · 2021: 17 · 2020: 6 · 2019: 21 · 2018: 5
FWCI
6.47
Type
journal article
Language
en
Open access
closed
Locations
  • Experimental Gerontology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Chongqing Health and Family Planning Commission (2015MSXM016)
  • Chongqing Development and Reform Commission ([2013]1420)
  • National Key Clinical Specialties Construction Program of China ([2013]544)
Topics
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
sarcopenia, physical activity, resveratrol, AMPK/SIRT1 pathway, aged rats, gastrocnemius, muscle mass, grip strength, muscle ultrastructure, sarcomere length, apoptosis, Bax/Bcl-2 ratio, combination therapy
MeSH terms
Resveratrol, Age Factors, Aging, Animals, Combined Modality Therapy, Disease Models, Animal, Exercise Therapy, Male, Physical Conditioning, Animal, Stilbenes, Time Factors, Signal Transduction, Tumor Suppressor Protein p53, Rats, Sprague-Dawley, Apoptosis, Muscle, Skeletal, Muscle Fibers, Skeletal, Proto-Oncogene Proteins c-bcl-2, bcl-2-Associated X Protein, Muscle Strength, AMP-Activated Protein Kinases, Sarcopenia, Sirtuin 1

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