Cognitive-enhancing and ameliorative effects of acanthoside B in a scopolamine-induced amnesic mouse model through regulation of oxidative/inflammatory/cholinergic systems and activation of the TrkB/CREB/BDNF pathway
Abstract
Recently, our research team reported the anti-amnesic potential of desalted-hydroethanolic extracts of Salicornia europaea L. (SE-EE). In this study, we performed bioactivity-guided isolation and identification of Acanthoside B (Aca.B), from SE-EE, as the potential bioactive candidate and examined anti-amnesic activity with its potential mechanism of action using an in vivo model. S7-L3-3 purified from SE-EE showed enhanced in vitro acetylcholinesterase (AChE) inhibitory activity. The isolated S7-L3-3 was identified and characterized as Aca.B using varied spectral analyses, i.e., Nuclear magnetic resonance (NMR), Ultraviolet-visible (UV-Vis), and Electrospray ionization-mass spectrometry (ESI-MS). In the in vitro studies, Aca.B exhibited negligible toxicity and showed a dose-dependent nitric oxide inhibitory potential in Lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. In the in vivo studies, the oral administration of Aca.B to mice showed enhanced bioavailability and dose-dependent repression of the behavioral/cognitive impairment by regulating the cholinergic function, restoring the antioxidant status, attenuating the inflammatory cytokines/mediators and actively enriching neurotropic proteins in the hippocampal regions of the scopolamine-administered mice.
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Salicornia europaea ethanol extract containing Acanthoside B at 10-20 mg/kg significantly decreased AChE levels in hippocampus and cortical regions compared to the scopolamine-treated group (p<0.05).
“oral administration (o.p.) of Salicornia europaea ethanol extract (SE-EE), which contains Acanthoside B (Aca.B), at doses of 10–20 mg/kg significantly decreased AChE levels in both the hippocampus and cortical regions compared to the scopolamine-treated group (p < 0.05) [27].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [27]
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Self-citation
- Authors
- Karthivashan, G., Kweon, M.-H., Park, S.-Y., Kim, J.-S., Kim, D.-H., Ganesan, P., & Choi, D.-K.
- Affiliations
- Konkuk University (KR)
- Corresponding author
- Choi, Dong-Kug
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2019-07
- Volume
- 129
- Pages
- 444-457
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W2944218266
- Cited by
- 34
- References
- 61
- Citations by year
- 2025: 5 · 2024: 3 · 2023: 9 · 2022: 6 · 2021: 7 · 2020: 3 · 2019: 1
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Konkuk University
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- Salicornia europaea, scopolamine-induced amnesia, antiamnesic activity, acetylcholinesterase inhibition, neuroinflammation, oxidative stress, nitric oxide, microglia, hippocampus, neuroprotection, bioactivity-guided isolation
- MeSH terms
- Amnesia, Animals, Disease Models, Animal, Furans, Glucosides, Inflammation, Membrane Glycoproteins, Oxidation-Reduction, Protein-Tyrosine Kinases, Receptors, Cholinergic, Scopolamine, Cyclic AMP Response Element-Binding Protein, Lignans, Brain-Derived Neurotrophic Factor, Receptor, trkB, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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