hScrib interacts with ZO-2 at the cell-cell junctions of epithelial cells
Abstract
In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins of the cell–cell junctions. We report the direct interaction between hScrib and ZO‐2, a junction‐associated protein. This interaction relies on two PDZ domains of hScrib and on the C‐terminal motif of ZO‐2. Both proteins localise at cell–cell junctions of epithelial cells. A point mutation in the LRR of hScrib delocalises the protein from the plasma membrane and abrogates the interaction with ZO‐2 but not with βPIX. Tyrosine phosphorylation of hScrib does not impair the interaction with ZO‐2. We show a direct link between two junctional proteins that are down‐regulated during cancer progression.
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In cultured mammalian cells and in the intestinal and cochlear epithelia in vivo, Scribble localizes to the basolateral membrane and appears to overlap adherens junctions, although its localization relative to tight junctions remains less clear.
“In cultured mammalian cells and in the intestinal and cochlear epithelia in vivo, Scribble localizes to the basolateral membrane and appears to overlap adherens junctions, although its localization relative to tight junctions remains less clear (Métais et al., 2005; Navarro et al., 2005; Montcouquiol et al., 2006; Ivanov et al., 2010; Yoshihara et al., 2011; Chen et al., 2016).”
From Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation (2019) · cites this paper as Métais et al., 2005
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“…Scribble localizes to the basolateral membrane and appears to overlap adherens junctions, although its localization relative to tight junctions remains less clear (Métais et al., 2005; Navarro et al., 2005; Montcouquiol et al., 2006; Ivanov et al., 2010; Yoshihara et al., 2011; Chen et al., 2016).”
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Self-citation
- Authors
- Métais, J.-Y., Navarro, C., Santoni, M.-J., Audebert, S., & Borg, J.-P.
- Affiliations
- Inserm (FR)
- Corresponding author
- Borg, Jean-Paul
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2005-06-13
- Volume
- 579
- Issue
- 17
- Pages
- 3725-3730
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1965912855
- Cited by
- 77
- References
- 39
- Citations by year
- 2024: 2 · 2023: 2 · 2022: 4 · 2021: 1 · 2020: 2 · 2019: 5 · 2018: 3 · 2017: 2 · 2015: 3 · 2014: 5 · 2013: 8 · 2012: 5
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hippo pathway signaling and YAP/TAZ
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- Scribble, ZO-2, PDZ domain, cell-cell contact, epithelial cells, septate junctions, protein-protein interaction, tyrosine phosphorylation, LRR domain, betaPIX, tumor progression, tumor suppressor
- MeSH terms
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Epithelial Cells, Humans, Intercellular Junctions, Membrane Proteins, Molecular Sequence Data, Phosphorylation, Tyrosine, Protein Structure, Tertiary, COS Cells, Tumor Suppressor Proteins, Protein Interaction Mapping, Zonula Occludens-2 Protein
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