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Cited by 28

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Heme‐dependent autophosphorylation of a heme sensor kinase, ChrS, from Corynebacterium diphtheriae reconstituted in proteoliposomes

Ito, Yoko, Nakagawa, Shoko, Komagata, Ayako, Ikeda-Saito, Masao, Shiro, Yoshitsugu, Nakamura, Hiro·2009·FEBS Letters

Abstract

Corynebacterium diphteriae employs the response regulator, ChrA, and the sensor kinase, ChrS, of a two‐component signal transduction system to utilize host heme iron. Although ChrS is predicted to encode a heme sensor, the sensing mechanism remains to be characterized. In this report, ChrS expressed in Eshcherichia coli membranes was solubilized and purified using decylmaltoside. ChrS protein incorporated into proteoliposomes catalyzed heme‐dependent autophosphorylation by ATP. Other metalloporphyrins and iron did not stimulate kinase activity. The UV–Vis spectrum of hemin in the ChrS–proteoliposomes indicated that heme directly interacts with ChrS. This is the first functional reconstitution of a bacterial heme‐sensing protein.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.febslet.2009.06.001

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

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Citations

Cites

Metadata

Authors
Ito, Y., Nakagawa, S., Komagata, A., Ikeda-Saito, M., Shiro, Y., & Nakamura, H.
Venue
FEBS Letters
Publisher
Wiley
Published
2009-06-08
Volume
583
Issue
13
Pages
2244-2248
ISSN
  • 0014-5793
  • 1873-3468
Cited by
28
References
33
Language
en

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.