Coaggregation ability of Weissella cibaria isolates with Fusobacterium nucleatum and their adhesiveness to epithelial cells
Abstract
In the present study, we evaluated the ability of Weissella cibaria isolated from the oral cavity to coaggregate with Fusobacterium nucleatum, and the adhesiveness of these strains to epithelial cells. W. cibaria efficiently coaggregated with F. nucleatum, and adhered to epithelial cells. We tested the effects of various factors on the coaggregation. The coaggregation and adhesiveness of W. cibaria disappeared upon exposure to pronase or LiCl, suggesting that proteinaceous components on the surface of W. cibaria mediated the coaggregation and adhesiveness. In conclusion, W. cibaria may serve as a potential probiotic with the ability to establish an oral flora protecting against oral pathogens.
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Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have demonstrated a capacity to aggregate with oral pathogens such as Fusobacterium nucleatum or Streptococcus mutans in vitro and in vivo.
“Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have been applied as probiotics and have demonstrated a capacity to aggregate with oral pathogens, such as Fusobacterium nucleatum or Streptococcus mutans, in vitro [8–10] and in vivo [11].”
From Coaggregation of oral pathogens by postbiotic lactobacilli (2025) · cites this paper as [8]
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
16S ribosomal DNA amplification for phylogenetic study
Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.2.697-703.1991 · Cited by 11,970 · see citing works
Rapid extraction of bacterial genomic DNA with guanidium thiocyanate
Pitcher, D. G., Saunders, N. A., & Owen, R. J. (1989) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1989.tb00262.x · Cited by 2,148 · see citing works
Lactic acid bacteria of foods and their current taxonomy
Stiles, M. E., & Holzapfel, W. H. (1997) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(96)01233-0 · Cited by 1,100 · see citing works
Oral Microbial Communities: Biofilms, Interactions, and Genetic Systems
Kolenbrander, P. E. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.413 · Cited by 894 · see citing works
Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum
Del Re, B., Sgorbati, B., Miglioli, M., & Palenzona, D. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1365-2672.2000.00845.x · Cited by 731 · see citing works
Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells
Scaletsky, I. C. A., SILVA, M. L. M., & Trabulsi, L. R. (1984) · Infection and Immunity · doi:10.1128/iai.45.2.534-536.1984 · Cited by 585 · see citing works
Crystalline surface layers on bacteria.
Sleytr, U., & Messner, P. (1983) · Annual Review of Microbiology · doi:10.1146/annurev.mi.37.100183.001523 · Cited by 565 · see citing works
Cisar, J. O., Kolenbrander, P. E., & McIntire, F. C. (1979) · Infection and Immunity · doi:10.1128/iai.24.3.742-752.1979 · Cited by 494 · see citing works
Björkroth, J. K., Schillinger, U., Geisen, R. M., Weiss, N., Hoste, B., Holzapfel, W. H., Korkeala, H. J., & Vandamme, P. A. R. (2002) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/00207713-52-1-141 · Cited by 290 · see citing works
Reid, G., McGroarty, J. A., Angotti, R., & Cook, R. L. (1988) · Canadian Journal of Microbiology · doi:10.1139/m88-063 · Cited by 239 · see citing works
Vuyst, L. D., Schrijvers, V., Paramithiotis, S., Hoste, B., Vancanneyt, M., Swings, J., Kalantzopoulos, G., Tsakalidou, E., & Messens, W. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.12.6059-6069.2002 · Cited by 201 · see citing works
Handley, P. S., Harty, D. W. S., Wyatt, J. E., Brown, C. R., Doran, J. P., & Gibbs, A. C. (1987) · Microbiology · doi:10.1099/00221287-133-11-3207 · Cited by 187 · see citing works
Gill, H. S., Shu, Q., Lin, H., Rutherfurd, K. J., & Cross, M. L. (2001) · Medical Microbiology and Immunology · doi:10.1007/s004300100095 · Cited by 163 · see citing works
Drago, L., Gismondo, M. R., Lombardi, A., de Haën, C., & Gozzini, L. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1997.tb12610.x · Cited by 158 · see citing works
Characterization of the aggregation promoting factor from Lactobacillus gasseri , avaginal isolate
Boris, S., Suárez, J. E., & Barbés, C. (1997) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1997.00250.x · Cited by 139 · see citing works
Schneitz, C., Nuotio, L., & Lounatma, K. (1993) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1993.tb03028.x · Cited by 121 · see citing works
Kolenbrander, P. E., Parrish, K., Andersen, R. N., & Greenberg, E. P. (1995) · Infection and Immunity · doi:10.1128/iai.63.12.4584-4588.1995 · Cited by 121 · see citing works
Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi.
Choi, H.-J., Cheigh, C.-I., Kim, S.-B., Lee, J.-C., Lee, D., Choi, S.-W., Park, J. M., & Pyun, Y. (2002) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/00207713-52-2-507 · Cited by 92 · see citing works
Babaahmady, K. G., Challacombe, S., Marsh, P. D., & Newman, H. N. (1997) · Caries Research · doi:10.1159/000016430 · Cited by 86 · see citing works
Characterization and identification of lactic acid bacteria in “morcilla de Burgos”
Santos, E. M., Jaime, I., Rovira, J., Lyhs, U., Korkeala, H. J., & Björkroth, J. K. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.04.021 · Cited by 86 · see citing works
Weiss, E. I., Shaniztki, B., Dotan, M., Ganeshkumar, N., Kolenbrander, P. E., & Metzger, Z. (2000) · Oral Microbiology and Immunology · doi:10.1034/j.1399-302x.2000.150606.x · Cited by 73 · see citing works
Messner, P., Allmaier, G. M., Schäffer, C., Wugeditsch, T., Lortal, S., König, H., Niemetz, R., & Dorner, M. (1997) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1997.tb00303.x · Cited by 67 · see citing works
Oral bacterial ecology: the molecular basis.
Kuramitsu, H. K., & Ellen, R. P. (2000) · Cited by 53 · see citing works
Analysis of S-layer proteins ofLactobacillus brevis
Yasui, T., Yoda, K., & Kamiya, T. (1995) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1995.tb07881.x · Cited by 43 · see citing works
Rosen, G., Nisimov, I., Helcer, M., & Sela, M. N. (2003) · Infection and Immunity · doi:10.1128/iai.71.6.3652-3656.2003 · Cited by 37 · see citing works
Coaggregation between bacterial species
Kinder, S. A., & Holt, S. C. (1994) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(94)36020-0 · Cited by 29 · see citing works
Metzger, Z., Featherstone, L. G., Ambrose, W. W., Trope, M., & Arnold, R. R. (2001) · Oral Microbiology and Immunology · doi:10.1034/j.1399-302x.2001.016003163.x · Cited by 19 · see citing works
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International Journal of Current Research
(2018) · International Journal of Current Research · doi:10.24941/ijcr · Cited by 1,547 · see citing works
Strauss, J., Kaplan, G. G., Beck, P. L., Rioux, K. P., Panaccione, R., DeVinney, R., Lynch, T., & Allen‐Vercoe, E. (2011) · Inflammatory Bowel Diseases · doi:10.1002/ibd.21606 · Cited by 574 · see citing works
Bacterial interactions in dental biofilm
Huang, R., Li, M., & Gregory, R. L. (2011) · Virulence · doi:10.4161/viru.2.5.16140 · Cited by 493 · see citing works
The genus Weissella: taxonomy, ecology and biotechnological potential
Fusco, V., Quero, G. M., Cho, G., Kabisch, J., Meske, D., Neve, H., Bockelmann, W., & Franz, C. M. A. P. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00155 · Cited by 465 · see citing works
Bacterial Interactions in Dental Biofilm Development
Hojo, K., Nagaoka, S., Ohshima, T., & Maeda, N. (2009) · Journal of Dental Research · doi:10.1177/0022034509346811 · Cited by 424 · see citing works
Probiotics: contributions to oral health
Meurman, J. H., & Stamatova, I. (2007) · Oral Diseases · doi:10.1111/j.1601-0825.2007.01386.x · Cited by 337 · see citing works
Haukioja, A. (2010) · European Journal of Dentistry · doi:10.1055/s-0039-1697851 · Cited by 239 · see citing works
Probiotics: health benefits in the mouth.
Stamatova, I., & Meurman, J. H. (2009) · PubMed · Cited by 151 · see citing works
Lim, H.-S., Yeu, J.-E., Hong, S.-P., & Kang, M. (2018) · Molecules · doi:10.3390/molecules23081984 · Cited by 73 · see citing works
Self-citationPresence of non-oral bacteria in the oral cavity
Zaatout, N. (2021) · Archives of Microbiology · doi:10.1007/s00203-021-02300-y · Cited by 73 · see citing works
Inter‐bacterial correlations in subgingival biofilms: a large‐scale survey
Loozen, G., Özçelik, O., Boon, N., Mol, A. D., Schoen, C., Quirynen, M., & Teughels, W. (2013) · Journal Of Clinical Periodontology · doi:10.1111/jcpe.12167 · Cited by 70 · see citing works
The Mechanisms of Probiotics, Prebiotics, Synbiotics, and Postbiotics in Oral Cancer Management
Fuad, A. S. M., Amran, N. A., Nasruddin, N. S., Burhanudin, N. A., Dashper, S. G., & Arzmi, M. H. (2022) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-022-09985-7 · Cited by 63 · see citing works
Kang, M., Jang, H.-S., Oh, J., Yang, K.-H., Choi, N., Lim, H.-S., & Kim, S.-M. (2009) · The Journal of Microbiology · doi:10.1007/s12275-009-0097-7 · Cited by 51 · see citing works
Self-citationOccurrence, Structure, Chemistry, Genetics, Morphogenesis, and Functions of S-Layers
Messner, P., Schäffer, C., Egelseer, E. M., & Sleytr, U. B. (2010) · Springer eBooks · doi:10.1007/978-3-642-05062-6_2 · Cited by 49 · see citing works
Nagaoka, S., Hojo, K., Murata, S., Mori, T., Ohshima, T., & Maeda, N. (2008) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2008.01092.x · Cited by 46 · see citing works
Lee, D.-S., Lee, S.-A., Kim, M., Nam, S., & Kang, M. (2020) · Journal of Medicinal Food · doi:10.1089/jmf.2019.4603 · Cited by 44 · see citing works
Self-citationThe importance of the S-layer on the adhesion and aggregation ability of Lactic acid bacteria
Alp, D., Kuleaşan, H., & Korkut Altıntaş, A. (2020) · Molecular Biology Reports · doi:10.1007/s11033-020-05430-6 · Cited by 42 · see citing works
Kang, M., Lim, H.-S., Kim, S.-M., Lee, H., & Oh, J. (2011) · Journal of Bacteriology and Virology · doi:10.4167/jbv.2011.41.1.9 · Cited by 42 · see citing works
Self-citationOral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D
Mann, S.-Y., Park, M. S., Johnston, T. V., Ji, G. E., Hwang, K. T., & Ku, S. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01563-w · Cited by 42 · see citing works
Meurman, J. H. (2009) · Elsevier eBooks · doi:10.1533/9781845696290.3.421 · Cited by 33 · see citing works
Bacteria in oral health – probiotics and prebiotics a review.
Sudhakar Reddy, R., La, S., Ramesh, T., Rajesh Singh, T., Vijayalaxmi, N., & Lavanya, R. (2011) · Cited by 33 · see citing works
Effect of Weissella cibaria on the reduction of periodontal tissue destruction in mice
Kim, J., Jung, B. H., Lee, J. H., Yoo, K., Lee, H., Kang, M., & Lee, J. (2020) · Journal of Periodontology · doi:10.1002/jper.19-0288 · Cited by 32 · see citing works
Self-citationPhylogenesis and Evolution of Lactic Acid Bacteria
Sun, Z., Yu, J. Z., Tong, D., Zhang, W., & Zhang, H. (2014) · Springer eBooks · doi:10.1007/978-94-017-8841-0_1 · Cited by 31 · see citing works
Mann, S.-Y., Park, M. S., Johnston, T. V., Ji, G. E., Hwang, K. T., & Ku, S. (2021) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-021-09761-z · Cited by 29 · see citing works
Do, K.-H., Park, H.-E., Kang, M., Kim, J., Yeu, J.-E., & Lee, W.-K. (2019) · Journal of Veterinary Dentistry · doi:10.1177/0898756419872562 · Cited by 29 · see citing works
Self-citationFhoula, I., Rehaiem, A., Najjari, A., Usai, D., Boudabous, A., Sechi, L. A., & Ouzari, H. (2018) · BioMed Research International · doi:10.1155/2018/1654151 · Cited by 28 · see citing works
Improvement of halitosis by probiotic bacterium Weissella cibaria CMU: A randomized controlled trial
Han, H., Yum, H., Cho, Y., & Kim, S. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1108762 · Cited by 29 · see citing works
Gut microbiota and the periodontal disease: role of hyperhomocysteinemia
Stanišić, D., Jovanović, M., George, A. K., Homme, R. P., Tyagi, N., Singh, M., & Tyagi, S. C. (2020) · Canadian Journal of Physiology and Pharmacology · doi:10.1139/cjpp-2020-0215 · Cited by 23 · see citing works
Dai, J., Chen, T., Guo, X., Dai, Z., He, Z., & Hu, Y. (2023) · Aquaculture · doi:10.1016/j.aquaculture.2023.739379 · Cited by 21 · see citing works
Developing Lactic Acid Bacteria as an Oral Healthy Food
Lai, W.-K., Lu, Y.-C., Hsieh, C.-R., Wei, C.-K., Tsai, Y., Chang, F., & Chan, Y. (2021) · Life · doi:10.3390/life11040268 · Cited by 17 · see citing works
The impact of probiotics on oral cancer: Mechanistic insights and therapeutic strategies
Priya, K. S. L., Datchanamoorthy, M., Ravi, S. S. S., Vajravelu, L. K., Lathakumari, R. H., & Malavika, G. (2025) · Oral Oncology Reports · doi:10.1016/j.oor.2025.100715 · Cited by 14 · see citing works
Zhang, J., & Duan, Z. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1000309 · Cited by 13 · see citing works
Kim, M.-J., Kim, W.-B., Koo, K. Y., Kim, B. R., Kim, D., Lee, S., Son, H. J., Hwang, D. Y., Kim, D. S., Lee, C. Y., et al. (2017) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1611.11051 · Cited by 12 · see citing works
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Golletz, P., Jensen, S. D., Collignon, M., Hall, C., Khamas, A. B., Møllebjerg, A., Schlafer, S., Meyer, R. L., & Tykwinska, K. (2025) · Journal of oral microbiology · doi:10.1080/20002297.2025.2508483 · Cited by 10 · see citing works
“Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have been applied as probiotics and have demonstrated a capacity to aggregate with oral pathogens, such as Fusobacterium nucleatum or Streptococcus mutans , in vitro [8–10] and in vivo [11].”
Macaya, C. C., Méndez, V., Durán, R. E., Aguila‐Torres, P., Salvà‐Serra, F., Jaén‐Luchoro, D., Moore, E. R. B., & Seeger, M. (2019) · Microbiology Resource Announcements · doi:10.1128/mra.00601-19 · Cited by 9 · see citing works
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Kang, M., Piao, M. S., Shin, B.-A., Lee, H., & Oh, J. (2006) · Journal of Bacteriology and Virology · doi:10.4167/jbv.2006.36.3.151 · Cited by 8 · see citing works
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Kang, M., Lim, H.-S., Kim, S.-M., Lim, Y.-J., Lee, H., & Oh, J. (2009) · Journal of Bacteriology and Virology · doi:10.4167/jbv.2009.39.4.295 · Cited by 8 · see citing works
Self-citationPark, H.-E., Kim, Y. J., Kim, M., Kim, H. R., Do, K.-H., Kim, J. K., Ham, J.-S., & Lee, W.-K. (2020) · Anaerobe · doi:10.1016/j.anaerobe.2020.102234 · Cited by 7 · see citing works
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Valencia‐Hernández, L. J., López‐López, K., & Serna‐Cock, L. (2016) · American Journal of Applied Sciences · doi:10.3844/ajassp.2016.1354.1364 · Cited by 7 · see citing works
Haukioja, A. L. (2009) · UTUPub (University of Turku) · Cited by 6 · see citing works
Preventive effects of Weissella cibaria CMU on the progression of periodontitis in a rat model
Do, K.-H., Boo, S., You, R. Y., Kim, S., Kim, S., Seo, K.-W., Kang, M., & Lee, W.-K. (2025) · Journal of Oral Microbiology · doi:10.1080/20002297.2025.2469895 · Cited by 5 · see citing works
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Ye, Q., Jia, D., Ji, J., Liu, Y., & Wu, G. (2024) · PLoS ONE · doi:10.1371/journal.pone.0298917 · Cited by 5 · see citing works
Baltakesmez, A., & Alp, D. (2025) · Journal of Sol-Gel Science and Technology · doi:10.1007/s10971-025-07012-z · Cited by 3 · see citing works
Lee, S.-K., Lim, K.-O., Yang, K.-I., You, S.-J., Kang, M.-S., & Lee, W. (2026) · BMC Oral Health · doi:10.1186/s12903-026-08003-4 · Cited by 3 · see citing works
Self-citationLactobacillus characterization and effects on oral biofilm composition
Vestman, N. R. (2013) · Cited by 3 · see citing works
Clinical significance of the genus Weissella: a brief review
Svishcheva, M. V., & Kolesnikova, E. A. (2025) · Clinical Microbiology and Antimicrobial Chemotherapy · doi:10.36488/cmac.2025.1.18-22 · Cited by 2 · see citing works
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Oranusi, S. U., Adedeji, O. M., & Olopade, B. K. (2014) · Covenant University Repository (Covenant University) · Cited by 2 · see citing works
Prebiotics and Probiotics and Oral Health
Meurman, J. H. (2009) · Springer eBooks · doi:10.1007/978-0-387-79058-9_28 · Cited by 1 · see citing works
Ribeiro, M., & Simões, M. V. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819497-3.00004-0 · Cited by 1 · see citing works
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Ye, Q., Jia, D., Ji, J., Liu, Y., & Wu, G. (2023) · Research Square · doi:10.21203/rs.3.rs-2847966/v1 · Cited by 1 · see citing works
Probiotics in Oral Biology and Dentistry
Meurman, J. H. (2014) · ASM Press eBooks · doi:10.1128/9781555815462.ch19
Oral Microfl ora, Oral Health and Probiotics
Verma, M. K., & Nawal, R. R. (2011) · doi:10.1201/b10770-13
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Verma, M. K., & Nawal, R. R. (2011) · Science Publishers eBooks · doi:10.1201/b10770-11
- Authors
- Kang, M.-S., Na, H.-S., & Oh, J.-S.
- Affiliations
- Chonnam National University (KR)
- Corresponding author
- Oh, Jong-Suk
- ORCID
- Kang, Mi-Sun
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-12
- Volume
- 253
- Issue
- 2
- Pages
- 323-329
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2018567182
- Cited by
- 53
- References
- 28
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 2
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Weissella cibaria, Fusobacterium nucleatum, bacterial coaggregation, bacterial adhesion, epithelial cells, oral cavity, probiotics, oral microbiota, surface proteins, oral pathogens
- MeSH terms
- Amino Acids, Bacillus, Bacterial Adhesion, Child, Child, Preschool, Electrophoresis, Polyacrylamide Gel, Epithelial Cells, Hot Temperature, Humans, Membrane Proteins, Mouth, Phylogeny, Pronase, RNA, Ribosomal, 16S, Bacterial Typing Techniques, Fusobacterium nucleatum, Sequence Analysis, DNA, Biofilms
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