Interactions between salivary Bifidobacterium adolescentis and other oral bacteria: in vitro coaggregation and coadhesion assays: Coaggregation and coadhesion of oral bifidobacteria
Abstract
Coaggregation assays were performed to investigate interactions between oral Bifidobacterium adolescentis and other oral bacterial species. Bifidobacterium adolescentis OLB6410 isolated from the saliva of healthy humans did not coaggregate with Actinomyces naeslundii JCM8350, Streptococcus mitis OLS3293, Streptococcus sanguinis JCM5708, Veillonella parvula ATCC17745 or Porphyromonas gingivalis OB7124, but it did coaggregate with Fusobacterium nucleatum JCM8532. Subsequent examination of biofilm formation on saliva-coated hydroxyapatite discs using FISH revealed that B. adolescentis OLB6410 could not directly adhere to the coated discs. It did, however, adhere to biofilms of A. naeslundii, V. parvula, and F. nucleatum, although it did not coaggregate with A. naeslundii nor with V. parvula. These results suggest that the adhesion of B. adolescentis to tooth surfaces is mediated by other oral bacteria. Heat- or proteinase K-treated F. nucleatum could not coaggregate with B. adolescentis. Similarly, the coaggregation and coadhesion of proteinase K-treated B. adolescentis were strongly inhibited. It is therefore probable that proteinaceous factors on the cellular surface of B. adolescentis and F. nucleatum are involved in their interaction. The data presented in this study add to our understanding of bifidobacterial colonization in the human oral cavity.
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Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have demonstrated a capacity to aggregate with oral pathogens such as Fusobacterium nucleatum or Streptococcus mutans in vitro and in vivo.
“Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have been applied as probiotics and have demonstrated a capacity to aggregate with oral pathogens, such as Fusobacterium nucleatum or Streptococcus mutans, in vitro [8–10] and in vivo [11].”
From Coaggregation of oral pathogens by postbiotic lactobacilli (2025) · cites this paper as [9]
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Coaggregation of oral pathogens by postbiotic lactobacilli
Golletz, P., Jensen, S. D., Collignon, M., Hall, C., Khamas, A. B., Møllebjerg, A., Schlafer, S., Meyer, R. L., & Tykwinska, K. (2025) · Journal of oral microbiology · doi:10.1080/20002297.2025.2508483 · Cited by 10 · see citing works
- Authors
- Nagaoka, S., Hojo, K., Murata, S., Mori, T., Ohshima, T., & Maeda, N.
- Affiliations
- Meiji (Japan) (JP)
- Tsurumi University (JP)
- Corresponding author
- Hojo, Kenichi
- ORCID
- Ohshima, Tomoko
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-04
- Volume
- 281
- Issue
- 2
- Pages
- 183-189
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W1838542403
- Cited by
- 46
- References
- 19
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 5 · 2014: 3 · 2012: 2
- FWCI
- 2.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- Bifidobacterium adolescentis, oral bacteria, co-aggregation, Fusobacterium nucleatum, Actinomyces naeslundii, Veillonella parvula, Streptococcus sanguinis, Streptococcus mitis, Porphyromonas gingivalis, biofilm formation, bacterial adhesion, saliva, oral colonization, proteinase K, heat treatment (food and biological processing)
- MeSH terms
- Bacteria, Bacterial Adhesion, Bifidobacterium, Hot Temperature, Humans, Mouth, Saliva, Fusobacterium nucleatum, In Situ Hybridization, Fluorescence, Bacterial Physiological Phenomena, Biofilms, Endopeptidase K
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