Prevention of peroxynitrite-induced oxidation and nitration reactions by ellagic acid
Abstract
Four benzoic acid derivatives and three phenylpropanoids were tested for their ability to suppress oxidation of dichlorodihydrofluorescein (DCDHF) induced by 3-morpholinosydnonimine (SIN-1, a peroxynitrite donor). Ellagic acid was found to potently inhibit this oxidation and to inhibit oxidation of DCDHF induced by peroxynitrite itself. Moreover, this compound prevented peroxynitrite-induced oxidative single strand breaks in pTZ 18U plasmid DNA and nitration of tyrosyl residues in bovine serum albumin. These results show that ellagic acid protects biomolecules against oxidative and nitrative damage induced by peroxynitrite in vitro.
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High concentrations of NO can react with superoxide anion to produce peroxynitrite, which is involved in several disorders including lipid peroxidation, membrane destabilization, oxidation, protein nitration, and DNA damage.
“In high concentrations NO can react with superoxide anion and produce potentially harmful peroxynitrite (Peluffo and Radi 2007), which is involved in the genesis of several disorders including induced lipid peroxidation, membrane destabilization (Virág et al. 2003), oxidation, protein nitration, and DNA damage (Ippoushi et al. 2009).”
From Evaluation of chemical constituents and antioxidant activity of coconut water (Cocus nucifera L.) and caffeic acid in cell culture (2013) · cites this paper as Ippoushi et al. 2009
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- Authors
- Ippoushi, K., Takeuchi, A., & Azuma, K.
- Affiliations
- National Agriculture and Food Research Organization (JP)
- Corresponding author
- Ippoushi, Katsunari
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2009-01
- Volume
- 112
- Issue
- 1
- Pages
- 185-188
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W2045615188
- Cited by
- 16
- References
- 19
- Citations by year
- 2023: 1 · 2021: 1 · 2017: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- ellagic acid, peroxynitrite, oxidative DNA damage, nitration, SIN-1, benzoic acid derivatives, phenylpropanoids, tyrosine nitration, bovine serum albumin, single-strand breaks, DNA protection, antioxidants
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