The Influence of Fermentation Condition on Production and Molecular Mass of EPS Produced by Streptococcus thermophilus 05-34 in Milk-Based Medium
Abstract
The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition. Structure analysis showed that EPS produced under the optimal fermentation condition was composed of galactose and glucose in a molar ratio of 1.0:0.8. This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change. Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide. These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
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MRS media supplemented with sucrose, fructose or maltose is generally used to screen HoPS production.
“For instance, de Man Rogosa and Sharpe (MRS) media supplemented with sucrose, fructose or maltose is generally used to screen HoPS production [37,55,56].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [37]
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“thermophilus 05-34 55–120 Skim milk Glucose or Galactose or Lactose or Sucrose or Fructose Glucose, Galactose [37]”
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- Authors
- Li, D., Li, J., Zhao, F., Wang, G., Qin, Q., & Hao, Y.
- Affiliations
- China Agricultural University (CN)
- Corresponding author
- Hao, Yanling
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2016-04
- Volume
- 197
- Issue
- Pt A
- Pages
- 367-372
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W2161644923
- Cited by
- 69
- References
- 26
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 11 · 2023: 7 · 2022: 4 · 2021: 10 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 3
- FWCI
- 5.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (21476250)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
- Keywords
- exopolysaccharides, Streptococcus thermophilus, fermentation optimization, molecular weight, degree of polymerization, rheological properties, starter lactic acid bacteria
- MeSH terms
- Animals, Cattle, Fermentation, Milk, Molecular Weight, Polysaccharides, Bacterial, Rheology, Streptococcus thermophilus
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