Sodium butyrate supplementation in high-soybean meal diets for turbot (Scophthalmus maximus L.): Effects on inflammatory status, mucosal barriers and microbiota in the intestine

Abstract

ABSTRACT A 12‐week feeding trial was conducted to evaluate the effects of dietary sodium butyrate (NaBT) on the intestinal health of juvenile turbot (Scophthalmus maximus L.), in terms of inflammatory status, mucosal barriers and microbiota. Three isonitrogenous and isolipidic practical diets were used: (1) fish meal based group (FM); (2) soybean meal group (SBM), soy protein replacing 40% fish meal protein in FM; (3) NaBT group, 0.2% NaBT supplemented in SBM. Each diet was fed to triplicate tanks (30 fish in each tank). The current results showed that 0.2% dietary NaBT improved the growth performance of fish and alleviated the enteropathy, increasing the absorptive surface and mitigating the infiltration of mixed leukocytes in lamina propria. Fish fed the NaBT diet presented increased activities of intestinal brush border enzyme and similar nutrient digestibility with the FM group. Compared to SBM, the inclusion of 0.2% NaBT in diet significantly up‐regulated the intestinal gene expression of tight junction proteins and down‐regulated the gene expression of TNF‐&agr; and NF‐&kgr;B. The gut microbial communities of the NaBT group were closer to the FM group than to the SBM group, in terms of PCoA, UPGMA and Heatmap analyses based on weighted Unifrac distance. The relative abundance of several dominant bacteria at the phylum (Proteobacteria, Bacteroidetes, Deinococcus‐Thermus and Actinobacteria) and genus level (Thermus, Acinetobacter, Bacteroides and Silanimonas) were altered by dietary NaBT. In conclusion, dietary NaBT had positive roles in protecting the intestinal health of turbot from the impairment of soybean meal. HighlightsSupplementing 0.2% dietary NaBT could alleviate soybean‐induced enteropathy of turbot.NaBT enhanced intestinal mucosal tight junction and relieved inflammation of turbot.NaBT in diet led to closer intestinal microbial composition to the fish fed fish meal.