Dietary administration of PVC and PE microplastics produces histological damage, oxidative stress and immunoregulation in European sea bass (Dicentrarchus labrax L.)

Abstract

Worldwide, plastic waste is increasingly being discharged into the oceans, where it breaks down into smaller particles. Of these particles, the ingestion of microplastics (MPs; particles smaller than 5 mm) have been documented in some aquatic animals, including fish, whose health and welfare suffer as a consequence. However, their precise effects are not completely understood. To shed light on this issue, European sea bass (Dicentrarchus labrax L.) specimens were fed diets containing 0 (control), 100 or 500 mg polyvinylchloride (PVC) or polyethylene (PE) MPs kg-1 diet for three weeks, after which samples of liver, intestine, skin mucus and head kidney (HK) were obtained. A histological study of the liver and intestine revealed important alterations in the fish fed the MP diets, compared with control fish. At a functional level, PE-MPs, but not PVC-MPs, decreased the activity of antioxidant enzymes, suggesting a certain level of oxidative stress. As regards immunity, the intake of PVC-MPs increased the phagocytic and respiratory burst activities of HK leucocytes whilst the intake of PE-MPs increased skin mucus immunoglobulin M levels and the respiratory burst activity of leucocytes. The results suggest that the short-medium term intake of PVC- or PE-MPs by fish slightly depresses their immunity and produces oxidative stress. However, based on the histological alterations found, it seems that longer exposure times might lead to irreversible damage that could compromise fish health and welfare.