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Nuclear factor interleukin 3 (NFIL3) participates in regulation of the NF-κB-mediated inflammation and antioxidant system in Litopenaeus vannamei under ammonia-N stress
Abstract
Nuclear factor interleukin 3 (NFIL3) is a critical upstream regulator of the NF-κB pathway. Nevertheless, the detailed molecular mechanism of NFIL3 and its function in shrimp have not been well characterized. In the present study, NFIL3 was identified and characterized from Litopenaeus vannamei. Molecular feature analysis revealed that the open reading frame (ORF) of LvNFIL3 was 2963 bp, which codes for a polypeptide of 516 amino acids with a conserved basic region leucine zipper (bZIP) domain. Sequence alignments and phylogenetic tree analysis showed that the amino acid sequence of LvNFIL3 shared 18.82%-98.07% identity with that of NFIL3 in other species, and was closely related to Penaeus monodon NFIL3. A core promoter in the 5' flanking region of LvNFIL3 was essential for regulation of transcription. LvNFIL3 mRNA was highly expressed in gills and hepatopancreas. Subcellular localization of the protein was observed almost exclusively in the nucleus. Amplification of mRNA by RT-qPCR showed that LvNFIL3 was induced in shrimp gills, hepatopancreas, and muscle after ammonia-N stress. Moreover, silencing of LvNFIL3 increased the mortality of shrimp exposed to ammonia-N. Furthermore, dual-luciferase reporter assay data suggested that LvNFIL3 was capable of activating the NF-κB pathway. Conversely, knockdown of LvNFIL3 decreased NF-κB homolog (Dorsal and Relish) and IkB homolog (Cactus) expression, as well as expression of anti-inflammatory cytokine (IL-16) and five antioxidant-re…
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“The suppression of pro-inflammatory genes was exemplified by the significant downregulation of both TLR4, a key activator of the immune response [65], and NFIL3, which is an upstream regulator of NF-κB-mediated inflammation [66] (Fig. 3E).”
From microRNA-132 attenuates inflammation in induced pluripotent stem cell-derived microglia from Alzheimer’s disease patients (2026) · cites this paper as [66]
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Proceedings of the National Academy of Sciences of the United States of America
Dowling, J. E., & Wald, G. (2009) · Nutrition Reviews · doi:10.1111/j.1753-4887.1981.tb06752.x · Cited by 17,893
T H 17 Cell Differentiation Is Regulated by the Circadian Clock
Yu, X., Rollins, D., Ruhn, K. A., Stubblefield, J. J., Green, C. B., Kashiwada, M., Rothman, P. B., Takahashi, J. S., & Hooper, L. V. (2013) · Science · doi:10.1126/science.1243884 · Cited by 441
Predicting Pol II Promoter Sequences using Transcription Factor Binding Sites
Prestridge, D. S. (1995) · Journal of Molecular Biology · doi:10.1006/jmbi.1995.0349 · Cited by 438
The nuclear signaling of NF-κB: current knowledge, new insights, and future perspectives
Wan, F., & Lenardo, M. J. (2010) · Cell Research · doi:10.1038/cr.2009.137 · Cited by 370
Hegazi, M. M., Attia, Z. I., & Ashour, O. A. (2010) · Aquatic Toxicology · doi:10.1016/j.aquatox.2010.04.007 · Cited by 304
Braissant, O., McLin, V. A., & Cudalbu, C. (2012) · Journal of Inherited Metabolic Disease · doi:10.1007/s10545-012-9546-2 · Cited by 288
Liang, Z., Liu, R., Zhao, D., Wang, L., Sun, M., Wang, M., & Song, L. (2016) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2016.05.009 · Cited by 286
Geiger, T. L., Abt, M. C., Gasteiger, G., Firth, M. A., O’Connor, M. H., Geary, C. D., O’Sullivan, T. E., van den Brink, M. R., Pamer, E. G., Hanash, A. M., et al. (2014) · Journal of Experimental Medicine · doi:10.1084/jem.20140212 · Cited by 269
Liu, Z., Huang, Y., Jiao, Y., Chen, Q., Wu, D., Yu, P., Li, Y., Cai, M., & Zhao, Y. (2020) · Aquatic Toxicology · doi:10.1016/j.aquatox.2020.105420 · Cited by 253
Research works printed but not linked: 2
- Si, 2019, Ammonia-N exposure alters neurohormone levels in the hemolymph and mRNA abundance of neurohormone receptors and associated downstream factors in the gills of Litopenaeus vannamei, J. Exp. Biol., 222
- Hidi, 2000, Role of B7-CD28/CTLA-4 costimulation and NF-κB in allergen-induced T cell chemotaxis by IL-16 and, RANTES, 164, 412
Cited by
Penning, A., Snoeck, S., Ormaechea, O. R., Ayyildiz, D., Polzer, O., Buitrago-Arango, M., Capobianco, R., de Winter, F., Balusu, S., Verhaagen, J., et al. (2026) · Acta Neuropathologica Communications · doi:10.1186/s40478-026-02228-8 · Cited by 6
Metadata
- Authors
- Zhuo, H., & Liu, J.
- ORCID
- Liu, Jianyong
- Venue
- Fish & Shellfish Immunology
- Publisher
- Elsevier BV
- Published
- 2022-12
- Volume
- 131
- Pages
- 1192-1205
- ISSN
- 1050-4648
- Cited by
- 19
- References
- 50
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Funders
- Ministry of Science and Technology of the People's Republic of China
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