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Polystyrene nanoplastic induces ROS production and affects the MAPK-HIF-1/NFkB-mediated antioxidant system in Daphnia pulex
Abstract
Recently, research on the biological effects of nanoplastics has grown exponentially. However, studies on the effects of nanoplastics on freshwater organisms and the mechanisms of the biological effects of nanoplastics are limited. In this study, the content of reactive oxygen species (ROS), gene and protein expression in the MAPK-HIF-1/NFkB pathway, and antioxidant gene expressions and enzyme activities were measured in Daphnia pulex exposed to polystyrene nanoplastic. In addition, the full-length extracellular signal-regulated kinases (ERK) gene, which plays an important role in the MAPK pathway, was cloned in D. pulex, and the amino acid sequence, function domain, and phylogenetic tree were analyzed. The results show that nanoplastic caused the overproduction of ROS along with other dose-dependent effects. Low nanoplastic concentrations (0.1 and/or 0.5 mg/L) significantly increased the expressions of genes of the MAPK pathway (ERK; p38 mitogen-activated protein kinases, p38; c-Jun amino-terminal kinases, JNK; and protein kinase B, AKT), HIF-1 pathway (prolyl hydroxylasedomain, PHD; vascular endothelial growth factor, VEGF; glucose transporter, GLUT; pyruvate kinase M, PKM; hypoxia-inducible factor 1, HIF1), and CuZn superoxide dismutase (SOD) along with the activity of glutathione-S-transferase. As the nanoplastic concentration increased, these indicators were significantly suppressed. The protein expression ratio of ERK, JNK, AKT, HIF1α, and NFkBp65 (nuclear transcription factor-kB p65) as well as the phosphorylation of ERK and NFkBp65 were increased in a dose-dependent manner. The activities of other antioxidant enzymes (catalase, total SOD, and CuZn SOD) were significantly decreased upon exposure to nanoplastic. Combined with our previous work, these results suggest that polystyrene nanoplastic causes the overproduction of ROS and activates the downstream pathway, resulting in inhibited growth, development, and reproduction. The present study fosters a better understanding of the biological effects of nanoplastics on zooplankton.
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Cites
Targeting HIF-1 for cancer therapy
Semenza, G. L. (2003) · Nature Reviews Cancer · doi:10.1038/nrc1187 · Cited by 6,717
Environmentally induced oxidative stress in aquatic animals
Lushchak, V. I. (2011) · Aquatic Toxicology · doi:10.1016/j.aquatox.2010.10.006 · Cited by 2,729
Interactions of microplastic debris throughout the marine ecosystem
Galloway, T. S., Cole, M., & Lewis, C. (2017) · Nature Ecology & Evolution · doi:10.1038/s41559-017-0116 · Cited by 1,955
Microplastic pollution in deep-sea sediments
Van Cauwenberghe, L., Vanreusel, A., Mees, J., & Janssen, C. R. (2013) · Environmental Pollution · doi:10.1016/j.envpol.2013.08.013 · Cited by 1,575
Emergence of Nanoplastic in the Environment and Possible Impact on Human Health
Lehner, R., Weder, C., Petri-Fink, A., & Rothen-Rutishauser, B. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.8b05512 · Cited by 1,242
Jeong, C.-B., Won, E.-J., Kang, H.-M., Lee, M.-C., Hwang, D.-S., Hwang, U.-K., Zhou, B., Souissi, S., Lee, S.-J., & Lee, J.-S. (2016) · Environmental Science & Technology · doi:10.1021/acs.est.6b01441 · Cited by 1,233
Physical Adsorption of Charged Plastic Nanoparticles Affects Algal Photosynthesis
Bhattacharya, P., Lin, S., Turner, J. P., & Ke, P. C. (2010) · The Journal of Physical Chemistry C · doi:10.1021/jp1054759 · Cited by 1,021
Bioavailability and effects of microplastics on marine zooplankton: A review
Botterell, Z. L. R., Beaumont, N., Dorrington, T., Steinke, M., Thompson, R. C., & Lindeque, P. K. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2018.10.065 · Cited by 966
Della Torre, C., Bergami, E., Salvati, A., Faleri, C., Cirino, P., Dawson, K. A., & Corsi, I. (2014) · Environmental Science & Technology · doi:10.1021/es502569w · Cited by 632
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Sangkham, S., Faikhaw, O., Munkong, N., Sakunkoo, P., Arunlertaree, C., Chavali, M., Mousazadeh, M., & Tiwari, A. (2022) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2022.113832 · Cited by 465
Huang, J.-N., Wen, B., Xu, L., Ma, H.-C., Li, X.-X., Gao, J.-Z., & Chen, Z.-Z. (2022) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.126830 · Cited by 202
Timilsina, A., Adhikari, K., Yadav, A. K., Joshi, P., Ramena, G., & Bohara, K. (2023) · Science of The Total Environment · doi:10.1016/j.scitotenv.2023.164999 · Cited by 104
Nogueira, D. J., Silva, A. C. D. O. D., da Silva, M. L. N., Vicentini, D. S., & Matias, W. G. (2022) · Science of The Total Environment · doi:10.1016/j.scitotenv.2021.151360 · Cited by 74
Zhuo, H., & Liu, J. (2022) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2022.11.028 · Cited by 19
Metadata
- Authors
- Liu, Z., Huang, Y., Jiao, Y., Chen, Q., Wu, D., Yu, P., Li, Y., Cai, M., & Zhao, Y.
- Venue
- Aquatic Toxicology
- Publisher
- Elsevier BV
- Published
- 2020-03
- Volume
- 220
- Pages
- 105420
- ISSN
- 0166-445X (ISSN-L)
- OpenAlex
- W3001331936
- Cited by
- 253
- References
- 9
- FWCI
- 8.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Liu, Z., Huang, Y., Jiao, Y., Chen, Q., Wu, D., Yu, P., Li, Y., Cai, M., & Zhao, Y. (2020). Polystyrene nanoplastic induces ROS production and affects the MAPK-HIF-1/NFkB-mediated antioxidant system in Daphnia pulex. Aquatic Toxicology. https://doi.org/10.1016/j.aquatox.2020.105420
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