Successful creation of pancreatic cancer organoids by means of EUS-guided fine-needle biopsy sampling for personalized cancer treatment
Open accessAbstract
Background and aims Pancreatic cancer organoids are tumor models of individualized human pancreatic ductal adenocarcinoma (PDA), created from surgical specimens and used for personalized treatment strategies. Unfortunately, most patients with PDA are not operative candidates. Creation of human PDA organoids at the time of initial tumor diagnosis is therefore critical. Our aim was to assess the feasibility of creating human PDA organoids by EUS fine-needle biopsy (EUS-FNB) sampling in patients with PDA.Methods In this prospective clinical trial in patients referred to evaluate a pancreatic mass, EUS-FNA was performed for initial onsite diagnosis. Two additional needle passes were performed with a 22-gauge FNB needle for organoid creation. Primary outcome was successful isolation of organoids within 2 weeks of EUS-FNB sampling (P0, no passages), confirmed by organoid morphology and positive genotyping.Results Thirty-seven patients with 38 PDA tumors were enrolled. Successful isolation of organoids (P0) was achieved in 33 of 38 tumors (87%). Establishment of PDA organoid lines for ≥5 passages of growth (P5, five passages) was reached in 25 of 38 tumors (66%). In the single patient with successful P5 FNB sampling-derived and P5 surgically derived organoids, there was identical matching of specimens. There were no serious adverse events. Two patients developed bleeding at the EUS-FNB puncture site requiring hemostasis clips.Conclusions Pancreatic cancer organoids can be successfully and rapidly created by means of EUS-FNB sampling using a 22-gauge needle at the time of initial diagnosis. Successful organoid generation is essential for precision medicine in patients with pancreatic cancer in whom most are not surgically resectable. (Clinical trial registration number: NCT03140592.).
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Tumor tissue from biopsies or surgical resections is typically processed similarly to normal tissue to isolate tumor cells for organoid growth.
“Tumor tissue, derived from either biopsies or surgical resections, is also typically processed akin to normal tissue to isolate tumour cells to grow as organoids16,49–51.”
From Organoids (2022) · cites this paper as [50]
The Methods (Organoid Generation and Maintenance; page unavailable) describe dissociating fine-needle biopsy tissue and growing the resulting cells and tissue fragments as organoids; they also say organoids were made from surgically resected tumors. The paper does not compare this processing with normal-tissue processing or establish that the approach is typical across tumor types. (LLM, conf=0.85)
Experimental — accuracy not yet measuredTumor cells from liquid samples such as peripheral blood, ascites, and pleural effusions can be used as starting material to generate organoids.
“Tumor cells isolated from liquid samples such as peripheral blood52, ascites50,53, and pleural effusions54 can also be used as starting material to generate organoids.”
From Organoids (2022) · cites this paper as [50]
Citation 53 is attached to ascites. The Methods (Organoid Generation and Maintenance; page unavailable) describe organoids grown from fine-needle biopsy tissue and surgically resected tumors, but our copy does not address generating organoids from ascites. Searches for ascites and other liquid samples found no supporting passage. (LLM, conf=0.76)
Experimental — accuracy not yet measured
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- Authors
- Tiriac, H., Bucobo, J. C., Tzimas, D., Grewel, S., Lacomb, J. F., Rowehl, L. M., Nagula, S., Wu, M., Kim, J., Sasson, A., Vignesh, S., Martello, L., Munoz-Sagastibelza, M., Somma, J., Tuveson, D. A., Li, E., & Buscaglia, J. M.
- Affiliations
- Stony Brook School (US)
- SUNY Downstate Health Sciences University (US)
- Cold Spring Harbor Laboratory (US)
- Corresponding author
- Buscaglia, Jonathan M.
- ORCID
- Venue
- Gastrointestinal Endoscopy
- Publisher
- Elsevier BV
- Published
- 2018-06
- Volume
- 87
- Issue
- 6
- Pages
- 1474-1480
- ISSN
- 0016-5107 (ISSN-L)
- PMC
- PMC6143289
- OpenAlex
- W2608725539
- Cited by
- 164
- References
- 30
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 20 · 2023: 12 · 2022: 31 · 2021: 26 · 2020: 30 · 2019: 17 · 2018: 9 · 2017: 2
- FWCI
- 7.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Gastrointestinal Endoscopy (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (submittedVersion)
- Funders
- Boston Scientific Corporation
- Stony Brook University
- National Institutes of Health (5p30ca045508-35)
- National Cancer Institute (P20CA192996, P50CA101955, R01CA188134, U10CA180944, P20CA192994, U01CA168409, P30-CA045508, R01CA190092)
- Topics
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- pancreatic ductal adenocarcinoma, pancreatic cancer organoids, EUS-guided fine-needle biopsy, precision oncology, precision medicine, unresectable pancreatic cancer, endoscopic ultrasound-guided fine-needle aspiration
- MeSH terms
- Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Organoids, Pancreatic Neoplasms, Tumor Cells, Cultured, Carcinoma, Pancreatic Ductal, Tissue Culture Techniques, Precision Medicine, Endoscopic Ultrasound-Guided Fine Needle Aspiration
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