Cited by 113biodegradable polymer synthesis and propertiesBiopolymer Synthesis and ApplicationsMicrobial bioremediation and biosurfactants

Poly-β-hydroxyalkanoates production from cassava starch hydrolysate by Cupriavidus sp. KKU38

Poomipuk, Natthaporn, Reungsang, Alissara, Plangklang, Pensri·2014·International Journal of Biological Macromolecules

Abstract

A bacterium capable of accumulating polyhydroxyalkanoates (PHAs), Cupriavidus sp. KKU38 (GenBank Accession no. AM260479), was isolated from cassava starch wastewater of cassava starch manufacturing process. KKU38 can utilize glucose, fructose, maltose and xylose for PHA production. Glucose was the most suitable sugar for PHA production giving the highest PHA content and yield of 73.88% and 0.16 g/g-total sugar consumed, respectively. Lactose and sucrose were not suitable carbon sources for both biomass and PHA productions. PHA production from cassava starch hydrolysate (CSH) by the strain KKU38 was maximized under N-limited conditions (COD:N:P of 100:1:2.43). The moderately high biomass concentrations of 5.97 g/L with PHA content and yield of 61.60% and 0.20 g/g-total sugar consumed, respectively, were obtained under the optimum conditions. The analysis of PHA produced under the optimum conditions by (1)H NMR, (13)C NMR and FT-IR indicated that the produced polymer is homopolymer polyhydroxybutyrate (PHB).

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Crossref · 10.1016/j.ijbiomac.2014.01.002

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Authors
Poomipuk, N., Reungsang, A., & Plangklang, P.
Affiliations
Khon Kaen University (TH)
Corresponding author
Reungsang, Alissara
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2014-04
Volume
65
Pages
51-64
ISSN
0141-8130 (ISSN-L)
OpenAlex
W2066371418
Cited by
113
References
56
Citations by year
2025: 11 · 2024: 12 · 2023: 16 · 2022: 15 · 2021: 18 · 2020: 16 · 2019: 8 · 2018: 5 · 2017: 6 · 2016: 1 · 2015: 4 · 2014: 1
FWCI
1.52
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Office of National Research Council of Thailand, Bangkok, Thailand
  • Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen, Thailand
Topics
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
Keywords
polyhydroxyalkanoates, polyhydroxybutyrate, PHA production, cassava starch wastewater, glucose utilization, xylose utilization, nitrogen limitation, carbon source screening, PHA yield, biopolymer characterization
MeSH terms
Bacterial Proteins, Manihot, Feasibility Studies, Genotype, Hydrolysis, Nitrogen, Phenotype, Phosphorus, RNA, Ribosomal, 16S, Sewage, Species Specificity, Starch, Cupriavidus, Polyhydroxyalkanoates

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