Quantification and evaluation of thymoquinone loaded mucoadhesive nanoemulsion for treatment of cerebral ischemia
Abstract
Stroke is an important cause of deaths worldwide, resulting in an irreversible deterioration of the central nervous system. Finally, production of more free radicals. Therefore, Thymoquinone is having antioxidant property and reported to have a potential role in the amelioration of cerebral ischemia but due to low solubility and poor absorption; they exhibit low serum and tissue levels. Present work aims to prepare nanoemulsions in order enhance the bioavailability of drug and hence evaluate the drug targeting in brain via non-invasive nasal route administration. Thymoquinone Mucoadhesive Nanoemulsion (TMNE) was prepared by ionic gelation method; characterized for particles size, entrapment efficiency, zeta potential, and ex vivo permeation study. Optimized TMNE ended up with a mean globule size 94.8±6.61nm; zeta potential -13.5±1.01mV; drug content 99.86±0.35% and viscosity 110±12cp. Ultra Performance Liquid Chromatography-Photodiode Array (UPLC-PDA) based bioanalytical method was developed and validated for pharmacokinetics, biodistribution, brain-targeting efficiency (628.5786±44.79%) and brain drug-targeting potential (89.97±2.94%) studies via post intranasal administration which revealed enhanced bioavailability of TQ in brain as compared to intravenous administration. Improved neurobehavioural activity (locomotor and grip strength) was observed in middle cerebral artery occlusion induced cerebral ischemic rats after i.n. administration of TMNE.
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Thymoquinone mucoadhesive nanoemulsions had a mean globule size of about 100 nm.
“The NEs had a mean globule size of about 100 nm.”
From Nanoemulsions for “Nose-to-Brain” Drug Delivery (2019) · cites this paper as [68]
Incorporation of chitosan increased the permeability of thymoquinone nanoemulsions across goat nasal mucosa.
“Ex vivo permeation studies across goat nasal mucosa were performed showing that the incorporation of chitosan increased the permeability of the NEs, confirming the penetration enhancing properties of this polymer due to its transient opening of tight junctions.”
From Nanoemulsions for “Nose-to-Brain” Drug Delivery (2019) · cites this paper as [68]
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Nanoemulsions for “Nose-to-Brain” Drug Delivery
Bonferoni, M. C., Rossi, S., Sandri, G., Ferrari, F., Gavini, E., Rassu, G., & Giunchedi, P. (2019) · Pharmaceutics · doi:10.3390/pharmaceutics11020084 · Cited by 269 · see citing works
- Authors
- Ahmad, N., Ahmad, R., Alam, M. A., Samim, M., Iqbal, Z., & Ahmad, F. J.
- Affiliations
- Imam Abdulrahman Bin Faisal University (SA)
- Jamia Hamdard (IN)
- Corresponding author
- Ahmad, Niyaz
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2016-07
- Volume
- 88
- Pages
- 320-332
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2293607107
- Cited by
- 110
- References
- 40
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 10 · 2023: 9 · 2022: 9 · 2021: 12 · 2020: 21 · 2019: 10 · 2018: 12 · 2017: 9 · 2016: 4
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- University of Dammam
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Health Sciences › Medicine › Complementary and alternative medicine › Nigella sativa pharmacological applications
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- thymoquinone, cerebral ischemia, stroke, ionic gelation, intranasal drug delivery, brain targeting, pharmacokinetics, tissue distribution, middle cerebral artery occlusion, antioxidants, neurobehavioral recovery, drug solubility enhancement
- MeSH terms
- Administration, Intranasal, Animals, Antioxidants, Behavior, Animal, Biological Availability, Brain Ischemia, Drug Carriers, Drug Compounding, Emulsions, Male, Particle Size, Psychomotor Performance, Tissue Distribution, Benzoquinones, Oxidative Stress, Neuroprotective Agents, Middle Cerebral Artery, Rats, Nanoparticles, Coronary Occlusion
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