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Cited by 20Bacterial Genetics and BiotechnologyProbiotics and Fermented FoodsAntimicrobial Resistance in Staphylococcus

Response of S. thermophilus LMD-9 to Bacitracin: Involvement of a BceRS/AB-like Module and of the Rhamnose-Glucose Polysaccharide Synthesis Pathway

Thevenard, B., Besset, C., Choinard, S., Fourcassié, P., Boyaval, P., Monnet, V., Rul, F.·2014·International Journal of Food Microbiology
DOI 10.1016/j.ijfoodmicro.2014.02.011
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Abstract

Streptococcus thermophilus is a lactic acid bacterium of major importance to the dairy industry as it is found in numerous cheeses and is one of the two bacterial species involved in the fermentation of yogurt. Bacterial two-component signal transduction systems (TCSs) play important roles in the process of bacterial environmental adaptation. S. thermophilus LMD-9 possesses eight such TCS systems; however, their functions have thus far been only poorly investigated. Here, we focused on two of the TCSs in LMD-9, TCS06 and TCS07, whose encoding genes are located close to each other on the chromosome, and are associated with those of ABC transporters. TCS06 homologs are frequently found in Lactobacillales, but their function has not yet been determined, while TCS07 and its upstream potential ABC transporter are homologous to the BceRS/AB system, which is involved in bacitracin resistance in Bacillus and Streptococcus species. To investigate the function(s) of TCS06 and TCS07, we constructed and characterized deletion mutants and performed transcriptional analysis in the presence and absence of bacitracin. We show here that both TCS06 and TCS07 regulate the genes in their close vicinity, in particular those encoding ABC transporters. We propose that the response of S. thermophilus to bacitracin includes i) a bacitracin export system, regulated by TCS07 and constituting a BceRS/AB-like detoxification module, and ii) the modification of cell-envelope properties via modulation of rhamnose-glucose polysaccharide synthesis, at least partially regulated by TCS06.

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  • CW-PS contain rhamnose and have multiple functions; in S. thermophilus they are involved in antibiotic stress response, while in Lact. lactis they act as phage receptors.

    “These molecules contain rhamnose and have multiple functions. In S. thermophilus, they are involved in antibiotic stress response, while in Lact. Lactis, they act as phage receptors [21,22,23].”

    From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [22]

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Authors
Thevenard, B., Besset, C., Choinard, S., Fourcassié, P., Boyaval, P., Monnet, V., & Rul, F.
Affiliations
  • AgroParisTech (FR)
  • DuPont (France) (FR)
Corresponding author
Rul, F.
ORCID
Venue
International Journal of Food Microbiology
Publisher
Elsevier BV
Published
2014-05
Volume
177
Pages
89-97
ISSN
0168-1605 (ISSN-L)
DOI
10.1016/j.ijfoodmicro.2014.02.011
OpenAlex
W2071420434
Cited by
20
References
39
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 3 · 2018: 1 · 2017: 3 · 2016: 3
FWCI
1.31
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Journal of Food Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
Keywords
Streptococcus thermophilus, bacitracin, two-component systems, ABC transporters, antibiotic resistance, deletion mutants, transcriptional regulation, lactic acid bacteria
MeSH terms
Anti-Bacterial Agents, Bacitracin, Genes, Bacterial, Microscopy, Electron, Scanning, Mutation, Polysaccharides, Bacterial, Rhamnose, Gene Expression Regulation, Bacterial, ATP-Binding Cassette Transporters, Gene Order, Drug Resistance, Bacterial, Microscopy, Electron, Transmission, Streptococcus thermophilus, Microbial Viability

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