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Cited by 174Mycotoxins in Agriculture and FoodNanoparticles: synthesis and applicationsAntifungal resistance and susceptibility

The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.

Antifungal activity of silver nanoparticles and simvastatin against toxigenic species of Aspergillus

Bocate, Karla Paiva, Reis, Guilherme Fonseca, de Souza, Patricia Canteri, Oliveira Junior, Admilton G., Durán, Nelson, Nakazato, Gerson, Furlaneto, Márcia Cristina, de Almeida, Ricardo Sérgio, Panagio, Luciano Aparecido·2019·International Journal of Food Microbiology
DOI 10.1016/j.ijfoodmicro.2018.11.012
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Abstract

Aspergillus spp. are ubiquitous fungi that grow on stored grains. Some species produce toxins that can harm human and animal health, leading to hepato- and nephrotoxicity, immunosuppression and carcinogenicity. Major fungicides used to prevent fungal growth may be toxic to humans and their repeated use over time increases levels of resistance by microorganisms. Nanotechnology is an emerging field that allows use of antimicrobial compounds in a more efficient manner. In this study, was evaluated the antifungal activity of biogenic silver nanoparticles (AgNPs, synthesized by fungi) and simvastatin (SIM, a semi-synthetic drug), alone and in combination against three toxigenic species belonging to the genera Aspergillus section Flavi (Aspergillus flavus, Aspergillus nomius and Aspergillus. parasiticus) and two of section Circumdati (Aspergillus ochraceus and Aspergillus melleus). SIM exhibited a MIC 50 of 78 μg/mL against species of Section Flavi and a MIC 50 of 19.5 μg/mL against species of Section Circumdati. The MIC 50 of AgNPs against Aspergillus flavus, Aspergillus nomius and Aspergillus parasiticus was 8 μg/mL, while the MIC 50 was 4 μg/mL against Aspergillus melleus and Aspergillus ochraceus. Checkerboard assay showed that these compounds, used alone and in combination, have synergistic and additive effects against toxicogenic species of Aspergillus. Analysis by SEM gives an idea of the effect of SIM and AgNPs alone and in combination on spore germination and vegetative growth. Ultrastructural analysis revealed that spore germination was prevented, or aberrant hyphae were formed with multilateral branches upon treatment with SIM and AgNPs. These results reveal potential benefits of using combination of AgNPs and SIM to control fungal growth.

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Crossref · 10.1016/j.ijfoodmicro.2018.11.012

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Metadata

Authors
Bocate, K. P., Reis, G. F., de Souza, P. C., Oliveira Junior, A. G., Durán, N., Nakazato, G., Furlaneto, M. C., de Almeida, R. S., & Panagio, L. A.
Affiliations
Universidade Estadual de Londrina (BR)
Corresponding author
Panagio, Luciano Aparecido
ORCID
Venue
International Journal of Food Microbiology
Publisher
Elsevier BV
Published
2019-02
Volume
291
Pages
79-86
ISSN
0168-1605 (ISSN-L)
DOI
10.1016/j.ijfoodmicro.2018.11.012
OpenAlex
W2901812483
Cited by
174
References
47
Citations by year
2026: 6 · 2025: 23 · 2024: 17 · 2023: 17 · 2022: 35 · 2021: 38 · 2020: 26 · 2019: 12
FWCI
16.79
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Journal of Food Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
silver nanoparticles, simvastatin, antifungal activity, Aspergillus flavus, Aspergillus nomius, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus melleus, mycotoxigenic fungi, synergistic effect, combination therapy, spore germination inhibition, hyphal ultrastructure, stored grain fungi
MeSH terms
Antifungal Agents, Aspergillus, Aspergillus flavus, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Silver, Biofilms, Simvastatin, Metal Nanoparticles

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