CoChat
Sign Up
Cited by 37Genetically Modified Organisms ResearchIdentification and Quantification in FoodAgricultural safety and regulations

Detection of genetically modified DNA sequences in milk from The Italian market

Agodi, Antonella, Barchitta, Martina, Grillo, Agata, Sciacca, Salvatore·2006·International Journal of Hygiene and Environmental Health
DOI 10.1016/j.ijheh.2005.08.005
Open this paper in CoChat — free account

Abstract

The possible transfer and accumulation of novel DNA and/or proteins in food for human consumption derived from animals receiving genetically modified (GM) feed is at present the object of scientific dispute. A number of studies failed to identify GM DNA in milk, meat, or eggs derived from livestock receiving GM feed ingredients. The present study was performed in order to: (i) develop a valid protocol by PCR and multicomponent analysis for the detection of specific DNA sequences in milk, focused on GM maize and GM soybean; (ii) assess the stability of transgenic DNA after pasteurization treatment and (iii) determine the presence of GM DNA sequences in milk samples collected from the Italian market. Results from the screening of 60 samples of 12 different milk brands demonstrated the presence of GM maize sequences in 15 (25%) and of GM soybean sequences in 7 samples (11.7%). Our screening methodology shows a very high sensitivity and the use of an automatic identification of the amplified products increases its specificity and reliability. Moreover, we demonstrated that the pasteurization process is not able to degrade the DNA sequences in spiked milk samples. The detection of GM DNA in milk can be interpreted as an indicator of fecal or airborne contamination, respectively, with feed DNA or feed particles, although an alternative source of contamination, possibly recognizable in the natural environment can be suggested. Further studies, performed on a larger number of milk samples, are needed to understand the likely source of contamination of milk collected from the Italian market.

Integrity signals

Registry

Crossref · 10.1016/j.ijheh.2005.08.005

Cites

14 of 14 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Neither recombinant DNA nor protein has ever been found in milk, meat, or eggs from animals that have eaten GE feed, with the exception of a single study that reported fragments of transgenic DNA in both 'organic' and 'conventional' milk in Italy.

    “however, neither recombinant DNA nor protein has ever been found in milk, meat, or eggs from animals that have eaten GE feed with the exception of a single study that reported the presence of fragments of transgenic DNA in both 'organic' and 'conventional' milk in Italy (Agodi et al., 2006).”

    From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Agodi et al., 2006

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Agodi, A., Barchitta, M., Grillo, A., & Sciacca, S.
Affiliations
University of Catania (IT)
Corresponding author
Agodi, Antonella
ORCID
Venue
International Journal of Hygiene and Environmental Health
Publisher
Elsevier BV
Published
2006-01
Volume
209
Issue
1
Pages
81-88
ISSN
1438-4639 (ISSN-L)
DOI
10.1016/j.ijheh.2005.08.005
OpenAlex
W1988481880
Cited by
37
References
14
Citations by year
2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 4 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 2 · 2012: 1
FWCI
1.60
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Journal of Hygiene and Environmental Health (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
Keywords
milk, transgenic maize, transgenic soybean, pasteurization, food contamination
MeSH terms
Animals, Zea mays, DNA, Recombinant, Food Analysis, Italy, Milk, Glycine max, Sterilization, Polymerase Chain Reaction, DNA, Plant, Food, Genetically Modified

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review