Persistence of plant DNA sequences in the blood of dairy cows fed with genetically modified (Bt176) and conventional corn silage
Abstract
To determine whether plant sequences, including transgenic sequences, are present in animal blood, we tested blood samples from Holstein cows fed with either Bt176 genetically modified corn or conventional corn. We used previously described sensitive real-time PCR assays targeting transgenic sequences (35S promoter and Bt176 specific junction sequence), a monocopy maize-specific sequence (ADH promoter), and two multicopy sequences from plant nucleus (26S rRNA gene) and chloroplast (psaB gene). The presence of Cry1A(b) protein in bovine blood samples was also tested using a sandwich ELISA kit. Our study shows the ability of plant nuclear and/or chloroplast DNA fragments to enter bovine blood circulation. However, maize nuclear DNA, both mono- and multicopy sequences, was less detected than chloroplast DNA, probably because the higher number of chloroplast copies and also possibly because nuclear DNA might be less protected by the nuclear membrane. Despite our data confirm the ability of small (ca.150 bp) plant DNA fragments to cross the intestinal barrier, we were unable to demonstrate clearly the presence of transgenic DNA or proteins in bovine blood. No sample tested positive with the two real-time PCR assays targeting transgenic sequences (35S promoter and Bt176 specific junction sequence). Only faint punctual positive results occurred randomly and were probably due to postsample collection or laboratory contamination or can be considered as artifact as they have never been confirmed. Our data highlight the difficulties to detect transgenic sequences in blood of dairy cows fed genetically modified corn (Bt176) silage. Those results show that in order to meet the consumers' demand of animals fed with GM products there is currently no cost-effective analytical procedure to replace documentary traceability.
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Given that there are no detectable and reliably quantifiable traces of GE materials in milk, meat, and eggs, any proposed labeling of animal products derived from GE-fed livestock would have to be based on documenting the absence of GE crops in the production chain.
“Given that there are no detectable and reliably quantifiable traces of GE materials in milk, meat, and eggs, any proposed labeling of animal products derived from GE-fed livestock would have to be based on documenting the absence of GE crops in the production chain, thereby necessitating the need for identity preservation and segregation requirements for producers and importers (Bertheau et al., 2”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Bertheau et al., 2009
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- Authors
- Bertheau, Y., Helbling, J. C., Fortabat, M. N., Makhzami, S., Sotinel, I., Audéon, C., Nignol, A. C., Kobilinsky, A., Petit, L., Fach, P., Brunschwig, P., Duhem, K., & Martin, P.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Corresponding author
- Bertheau, Y.
- Martin, P.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-01-05
- Volume
- 57
- Issue
- 2
- Pages
- 509-516
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2089932647
- Cited by
- 20
- References
- 32
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 2 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
- Keywords
- transgenic maize, dairy cattle, blood, corn silage, chloroplast DNA, 26S rDNA, transgene persistence, intestinal barrier, GMO traceability, Holstein cows
- MeSH terms
- Animals, Cattle, Zea mays, Female, Plant Proteins, Silage, DNA, Plant, Plants, Genetically Modified
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