Protein derivatization and sequential ion/ion reactions to enhance sequence coverage produced by electron transfer dissociation mass spectrometry
Open accessAbstract
Previously, we described implementation of a front-end ETD (electron transfer dissociation) source for an Orbitrap instrument (1). This source facilitates multiple fills of the C-trap with product ions from ETD of intact proteins prior to mass analysis. The result is a dramatic enhancement of the observed ion current without the need for time consuming averaging of data from multiple mass measurements. Here we show that ion-ion proton transfer (IIPT) reactions can be used to simplify ETD spectra and to disperse fragment ions over the entire mass range in a controlled manner. We also show that protein derivatization can be employed to selectively enhance the sequence information observed at the N- and C-termini of a protein.
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Ion-ion proton transfer reactions have been used mainly to reduce the complexity of ETD fragmentation spectra by shifting charge-reduced product ions to higher m/z values where signal overlap is limited.
“ion–ion proton transfer reactions have found several applications, most importantly to reduce the complexity of fragmentation spectra generated by electron transfer dissociation (ETD) by distributing charge-reduced product ions toward higher m/z values, where signal overlap is limited.32-34”
From Proton Transfer Charge Reduction Enables High-Throughput Top-Down Analysis of Large Proteoforms (2019) · cites this paper as [32]
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Self-citation
- Authors
- Anderson, L. C., English, A. M., Wang, W.-H., Bai, D. L., Shabanowitz, J., & Hunt, D. F.
- Affiliations
- University of Virginia (US)
- Corresponding author
- Hunt, Donald F.
- Venue
- International Journal of Mass Spectrometry
- Publisher
- Elsevier BV
- Published
- 2015-02
- Volume
- 377
- Pages
- 617-624
- ISSN
- 1387-3806 (ISSN-L)
- PMC
- PMC4379715
- OpenAlex
- W2048083804
- Cited by
- 37
- References
- 16
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 5 · 2023: 3 · 2021: 4 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 4 · 2016: 4 · 2015: 5
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- International Journal of Mass Spectrometry (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health Grants (GM37537, AI33993)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- electron transfer dissociation, protein derivatization, sequencing depth, protein sequencing, N-terminus, C-terminus, Orbitrap
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