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Cited by 480Mesenchymal stem cell researchReconstructive Surgery and Microvascular TechniquesBone Tissue Engineering Materials

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Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells

Mesimäki, K., Lindroos, B., Törnwall, J., Mauno, J., Lindqvist, C., Kontio, R., Miettinen, S., Suuronen, R.·2009·International Journal of Oral and Maxillofacial Surgery
DOI 10.1016/j.ijom.2009.01.001
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Abstract

Microvascular reconstruction is the state-of-the-art in many fields of defect surgery today. Currently, reconstruction of large bony defects involves harvesting of autologous bone causing donor site morbidity and risk of infection. Specifically, utilizing autologous adipose stem cells (autoASCs), large quantities of cells can be retrieved for cell therapy applications and the risk of tissue rejection is diminished. The authors describe the first case report of a microvascular custom-made ectopic bone flap employing good manufacturing practice (GMP) level ASCs. The patient underwent a hemimaxillectomy due to a large keratocyst. After 36 months of follow-up, the defect was reconstructed with a microvascular flap using autoASCs, beta-tricalcium phosphate and bone morphogenetic protein-2. ASCs were isolated and expanded in clean room facilities according to GMP standards and were characterized in vitro. After 8 months of follow-up, the flap had developed mature bone structures and vasculature and was transplanted into the defect area. Postoperative healing has been uneventful, and further rehabilitation with dental implants has been started. The in vitro characterization demonstrated multipotentiality and mesenchymal stem cell characteristics in ASCs. This is the first clinical case where ectopic bone was produced using autoASCs in microvascular reconstruction surgery and it will pave way for new clinical trials in the field.

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Metadata

Authors
Mesimäki, K., Lindroos, B., Törnwall, J., Mauno, J., Lindqvist, C., Kontio, R., Miettinen, S., & Suuronen, R.
Affiliations
  • Helsinki University Hospital (FI)
  • Tampere University (FI)
  • University of Helsinki (FI)
Corresponding author
Suuronen, R.
ORCID
Venue
International Journal of Oral and Maxillofacial Surgery
Publisher
Elsevier BV
Published
2009-03
Volume
38
Issue
3
Pages
201-209
ISSN
0901-5027 (ISSN-L)
DOI
10.1016/j.ijom.2009.01.001
OpenAlex
W2057425253
Cited by
480
References
0
Citations by year
2026: 7 · 2025: 12 · 2024: 6 · 2023: 21 · 2022: 21 · 2021: 20 · 2020: 26 · 2019: 31 · 2018: 30 · 2017: 35 · 2016: 36 · 2015: 36 · 2014: 46 · 2013: 47 · 2012: 42
FWCI
16.71
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Journal of Oral and Maxillofacial Surgery (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Health Sciences › Medicine › Surgery › Reconstructive Surgery and Microvascular Techniques
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
maxillary reconstruction, ectopic bone formation, adipose-derived stem cells, autologous stem cell therapy, microvascular reconstruction, hemimaxillectomy, keratocyst, beta-tricalcium phosphate, BMP-2, good manufacturing practices, mesenchymal stem cells, dental implants
MeSH terms
Adipose Tissue, Aged, Bone and Bones, Calcium Phosphates, Cell Differentiation, Humans, Male, Maxillary Diseases, Odontogenic Cysts, Ossification, Heterotopic, Osteocytes, Osteogenesis, Surgical Flaps, Treatment Outcome, Bone Substitutes, Oral Surgical Procedures, Plastic Surgery Procedures, Prostheses and Implants, Tissue Engineering, Multipotent Stem Cells, Abdominal Fat, Adult Stem Cells, Bone Morphogenetic Protein 2, Microvessels

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