Cited by 23Radiation Therapy and DosimetryNanoparticle-Based Drug DeliveryNanoplatforms for cancer theranostics

Zinc nanoparticles coated with doxorubicin-conjugated alginate as a radiation sensitizer in triple-negative breast cancer cells

Asadi, Niloofar, Gharbavi, Mahmoud, Rezaeejam, Hamed, Farajollahi, Alireza, Johari, Behrooz·2024·International Journal of Pharmaceutics

Abstract

The main treatment modalities for breast cancer include surgery, chemotherapy, and radiotherapy, and each treatment will bring different side effects. Design and synthesizing a novel nanostructure for chemo-radiotherapy has been proposed as an effective method in consideration to enhance the drug efficiency as well as improve the effect of radiotherapy. This study aimed to synthesize zinc nanoparticles (ZnNPs) coated with alginate conjugated with Doxorubicin (Dox) drug and investigate its effects along with X-irradiation on MDA-MB-231 triple-negative breast cancer cell line. ZnNPs coated with alginate were synthesized and conjugated to Dox by covalent bonding and characterized using various physicochemical tests. A hemolysis test was used to assess blood biocompatibility. The radiosensitization properties and anti-cancer effects of the synthesized nanostructures were tested by cell uptake, cell viability, apoptosis, cell cycle, and scratch assays with and without radiation exposure. The physicochemical characterization results showed that the synthesis of nanostructures was successfully carried out. The obtained results from the cell uptake assay showed the effective absorption of nanostructures by the cells. The Zn@Alg-Dox NPs significantly reduced cell growth, increased apoptosis, inhibited cell migration, and led to the arrest of different cell cycle phases in both conditions with and without X-ray exposure. Coating ZnNPs with alginate and Doxorubicin conjugation leads to an increase the radiation sensitivity in radiotherapy as well as therapeutic efficiency. Therefore, Zn@Alg-Dox NPs can be used as radiosensitizing nanomedicine for in vivo studies in the future.

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Crossref · 10.1016/j.ijpharm.2024.124285

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Authors
Asadi, N., Gharbavi, M., Rezaeejam, H., Farajollahi, A., & Johari, B.
Affiliations
  • Tabriz University of Medical Sciences (IR)
  • Ahvaz Jundishapur University of Medical Sciences (IR)
  • Zanjan University of Medical Sciences (IR)
Corresponding author
  • Farajollahi, Alireza
  • Johari, Behrooz
Venue
International Journal of Pharmaceutics
Publisher
Elsevier BV
Published
2024-06
Volume
659
Pages
124285
ISSN
0378-5173 (ISSN-L)
OpenAlex
W4399117900
Cited by
23
References
58
Citations by year
2026: 5 · 2025: 17 · 2024: 1
FWCI
6.68
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Pharmaceutics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Tabriz University of Medical Sciences
  • Drug Applied Research Center, Tabriz University of Medical Sciences (68532, IR.TBZMED.VCR.REC.1400.543)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Radiation Therapy and Dosimetry
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
Keywords
zinc nanoparticles, doxorubicin, alginate coating, radiosensitization, triple-negative breast cancer, MDA-MB-231 cells, chemoradiotherapy, X-ray irradiation, apoptosis, cell cycle arrest, cell migration inhibition
MeSH terms
Alginates, Antibiotics, Antineoplastic, Cell Movement, Cell Survival, Doxorubicin, Female, Hemolysis, Humans, Radiation-Sensitizing Agents, Zinc, Apoptosis, Cell Line, Tumor, Metal Nanoparticles, Triple Negative Breast Neoplasms

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