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Cited by 268Cell Adhesion Molecules ResearchChemokine receptors and signalingCellular Mechanics and Interactions

Lymphocyte Crawling and Transendothelial Migration Require Chemokine Triggering of High-Affinity LFA-1 Integrin

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Shulman, Ziv, Shinder, Vera, Klein, Eugenia, Grabovsky, Valentin, Yeger, Orna, Geron, Erez, Montresor, Alessio, Bolomini-Vittori, Matteo +5 more·2009·Immunity
DOI 10.1016/j.immuni.2008.12.020PMC PMC2803105
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Abstract

Endothelial chemokines are instrumental for integrin-mediated lymphocyte adhesion and transendothelial migration (TEM). By dissecting how chemokines trigger lymphocyte integrins to support shear-resistant motility on and across cytokine-stimulated endothelial barriers, we found a critical role for high-affinity (HA) LFA-1 integrin in lymphocyte crawling on activated endothelium. Endothelial-presented chemokines triggered HA-LFA-1 and adhesive filopodia at numerous submicron dots scattered underneath crawling lymphocytes. Shear forces applied to endothelial-bound lymphocytes dramatically enhanced filopodia density underneath crawling lymphocytes. A fraction of the adhesive filopodia invaded the endothelial cells prior to and during TEM and extended large subluminal leading edge containing dots of HA-LFA-1 occupied by subluminal ICAM-1. Memory T cells generated more frequent invasive filopodia and transmigrated more rapidly than their naive counterparts. We propose that shear forces exerted on HA-LFA-1 trigger adhesive and invasive filopodia at apical endothelial surfaces and thereby promote lymphocyte crawling and probing for TEM sites.

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  • Both leukocytes and the endothelium become activated, causing upregulation of cell surface receptors mediating leukocyte-endothelial adhesion.

    “Both leukocytes and the endothelium become activated, causing upregulation of cell surface receptors mediating leukocyte-endothelial adhesion (215, 216, 537).”

    From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [537]

    The Discussion (page not available) describes chemokine-triggered activation of T-cell integrins and adhesion to cytokine-activated endothelial cells bearing ICAM-1 and VCAM-1. The Results (page not available) describe clustering of endothelial ICAM-1 beneath crawling T cells. These passages support activation and adhesion, but not the claim that activation of both cell types causes upregulation of cell-surface adhesion receptors. (LLM, conf=0.82)

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Cited by

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Authors
Shulman, Z., Shinder, V., Klein, E., Grabovsky, V., Yeger, O., Geron, E., Montresor, A., Bolomini-Vittori, M., Feigelson, S. W., Kirchhausen, T., Laudanna, C., Shakhar, G., & Alon, R.
Affiliations
  • Weizmann Institute of Science (IL)
  • University of Verona (IT)
  • Harvard University (US)
Corresponding author
Alon, Ronen
ORCID
Venue
Immunity
Publisher
Elsevier BV
Published
2009-03
Volume
30
Issue
3
Pages
384-396
ISSN
1074-7613 (ISSN-L)
DOI
10.1016/j.immuni.2008.12.020
PMC
PMC2803105
OpenAlex
W2101026205
Cited by
268
References
1
Citations by year
2026: 3 · 2025: 11 · 2024: 11 · 2023: 13 · 2022: 6 · 2021: 11 · 2020: 9 · 2019: 15 · 2018: 19 · 2017: 14 · 2016: 21 · 2015: 20 · 2014: 18 · 2013: 17 · 2012: 21
FWCI
20.92
Type
journal article
Language
en
Open access
Licence
Locations
  • Immunity (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Goethe-Universität Frankfurt am Main
  • Minerva Foundation
  • Israel Science Foundation
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
LFA-1 integrin, lymphocyte transendothelial migration, shear force, ICAM-1, memory T cells, naive T cells, lymphocyte adhesion, endothelial barrier
MeSH terms
Cell Movement, Cells, Cultured, Endothelium, Vascular, Humans, T-Lymphocytes, Lymphocyte Function-Associated Antigen-1, Intercellular Adhesion Molecule-1, Chemokines

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