Comparison of Immortalized bEnd5 and Primary Mouse Brain Microvascular Endothelial Cells as in vitro Blood–Brain Barrier Models for the Study of T Cell Extravasation

Structure and function of the blood–brain barrier

Abbott, N. J., Patabendige, A. A. K., Dolman, D. E. M., Yusof, S. R., & Begley, D. J. (2010) · Neurobiology of Disease · doi:10.1016/j.nbd.2009.07.030 · Cited by 5,185 · see citing works

Getting to the site of inflammation: the leukocyte adhesion cascade updated

Ley, K., Laudanna, C., Cybulsky, M. I., & Nourshargh, S. (2007) · Nature Reviews Immunology · doi:10.1038/nri2156 · Cited by 4,354 · see citing works

Untitled work

Vestweber (2008) · doi:10.1161/atvbaha.107.158014 · Cited by 806 · see citing works

Effector T cell interactions with meningeal vascular structures in nascent autoimmune CNS lesions.

Bartholomäus, I., Kawakami, N., Odoardi, F., Schläger, C., Miljkovic, D., Ellwart, J. W., Klinkert, W. E. F., Flügel-Koch, C., Issekutz, T. B., Wekerle, H., et al. (2009) · Nature · doi:10.1038/nature08478 · Cited by 685 · see citing works

Endothelial adherens junctions control tight junctions by VE-cadherin-mediated upregulation of claudin-5

Taddei, A., Giampietro, C., Conti, A., Orsenigo, F., Breviario, F., Pirazzoli, V., Potente, M., Daly, C., Dimmeler, S., & Dejana, E. (2008) · Nature Cell Biology · doi:10.1038/ncb1752 · Cited by 648 · see citing works

Permeability Studies on In Vitro Blood–Brain Barrier Models: Physiology, Pathology, and Pharmacology

Deli, M. A., Ábrahám, C. S., Kataoka, Y., & Niwa, M. (2005) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-004-1377-8 · Cited by 643 · see citing works

Regulation of Endothelial Junctional Permeability

Vandenbroucke, E. E., Mehta, D., Minshall, R. D., & Malik, A. B. (2008) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1420.016 · Cited by 598 · see citing works

Transcellular Diapedesis Is Initiated by Invasive Podosomes

Carman, C. V., Sage, P. T., Sciuto, T. E., de la Fuente, M. Á., Geha, R. S., Ochs, H. D., Dvorak, H. F., Dvořàk, A. M., & Springer, T. A. (2007) · Immunity · doi:10.1016/j.immuni.2007.04.015 · Cited by 497 · see citing works

Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola- and F-actin-rich domains

Millán, J., Hewlett, L. J., Glyn, M., Toomre, D. K., Clark, P., & Ridley, A. J. (2006) · Nature Cell Biology · doi:10.1038/ncb1356 · Cited by 416 · see citing works

Shear forces promote lymphocyte migration across vascular endothelium bearing apical chemokines

Cinamon, G., Shinder, V., & Alon, R. (2001) · Nature Immunology · doi:10.1038/88710 · Cited by 402 · see citing works

In vitro model for evaluating drug transport across the blood–brain barrier

Cecchelli, R., Dehouck, B., Descamps, L., Fénart, L., Buée‐Scherrer, V., Duhem, C., Lundquist, S., Rentfel, M., Torpier, G., & Dehouck, M. (1999) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(98)00083-0 · Cited by 383 · see citing works

Effect of venous shear stress on CD18-mediated neutrophil adhesion to cultured endothelium

Lawrence, M., Smith, C., Eskin, S. G., et al. (1990) · Blood · doi:10.1182/blood.v75.1.227.227 · Cited by 357 · see citing works

α4-integrin-VCAM-1 binding mediates G protein–independent capture of encephalitogenic T cell blasts to CNS white matter microvessels

Vajkoczy, P., Laschinger, M., & Engelhardt, B. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12440 · Cited by 357 · see citing works

Mini‐review: Transendothelial migration of leukocytes: through the front door or around the side of the house?

Engelhardt, B., & Wolburg, H. (2004) · European Journal of Immunology · doi:10.1002/eji.200425327 · Cited by 334 · see citing works

Epithelial transport and barrier function in occludin-deficient mice

Schulzke, J., Gitter, A. H., Mankertz, J., Spiegel, S., Seidler, U. E., Amasheh, S., Saitou, M., Tsukita, S., & Fromm, M. (2005) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2005.01.008 · Cited by 320 · see citing works

Lymphocyte Crawling and Transendothelial Migration Require Chemokine Triggering of High-Affinity LFA-1 Integrin

Shulman, Z., Shinder, V., Klein, E., Grabovsky, V., Yeger, O., Geron, E., Montresor, A., Bolomini-Vittori, M., Feigelson, S. W., Kirchhausen, T., et al. (2009) · Immunity · doi:10.1016/j.immuni.2008.12.020 · Cited by 268 · see citing works

β1 Integrin deletion from the basal compartment of the mammary epithelium affects stem cells

Hosseraye, I. T.-D. L., Deugnier, M., Faraldo, M. M., Petit, V., Bouvard, D., Medina, D., Fässler, R., Thiery, J. P., & Glukhova, M. A. (2008) · Nature Cell Biology · doi:10.1038/ncb1734 · Cited by 264 · see citing works

Organization and signaling of endothelial cell-to-cell junctions in various regions of the blood and lymphatic vascular trees

Dejana, E., Orsenigo, F., Molendini, C., Bałuk, P., & McDonald, D. M. (2008) · Cell and Tissue Research · doi:10.1007/s00441-008-0694-5 · Cited by 211 · see citing works

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes

Smith, A. M., Carrasco, Y. R., Stanley, P., et al. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200412032 · Cited by 208 · see citing works

The development of experimental autoimmune encephalomyelitis in the mouse requires alpha4-integrin but not alpha4beta7-integrin.

Engelhardt, B., Laschinger, M., Schulz, M., Samulowitz, U., Vestweber, D., & Hoch, G. (1998) · Journal of Clinical Investigation · doi:10.1172/jci4271 · Cited by 203 · see citing works

Mechanisms for transcellular diapedesis: probing and pathfinding by `invadosome-like protrusions'

Carman, C. V. (2009) · Journal of Cell Science · doi:10.1242/jcs.047522 · Cited by 198 · see citing works

The role of cytoskeleton in the regulation of vascular endothelial barrier function

Bogatcheva, N. V., & Verin, A. D. (2008) · Microvascular Research · doi:10.1016/j.mvr.2008.06.003 · Cited by 194 · see citing works

Mouse syngenic in vitro blood–brain barrier model: a new tool to examine inflammatory events in cerebral endothelium

Coisne, C., Dehouck, L., Faveeuw, C., Delplace, Y., Miller, F., Landry, C., Morissette, C., Fénart, L., Cecchelli, R., Tremblay, P. J., et al. (2005) · Laboratory Investigation · doi:10.1038/labinvest.3700281 · Cited by 183 · see citing works

Immune cell entry into the central nervous system: Involvement of adhesion molecules and chemokines

Engelhardt, B. (2008) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2008.05.019 · Cited by 179 · see citing works

Phosphorylation of vascular endothelial cadherin controls lymphocyte emigration

Turowski, P., Martinelli, R., Crawford, R., Wateridge, D., Papageorgiou, A.-P., Lampugnani, M. G., Gamp, A. C., Vestweber, D., Adamson, P., Dejana, E., et al. (2008) · Journal of Cell Science · doi:10.1242/jcs.022681 · Cited by 169 · see citing works

Interaction of α4-integrin with VCAM-1 is involved in adhesion of encephalitogenic T cell blasts to brain endothelium but not in their transendothelial migration in vitro

Laschinger, M., & Engelhardt, B. (2000) · Journal of Neuroimmunology · doi:10.1016/s0165-5728(99)00156-3 · Cited by 149 · see citing works

T-cell interaction with ICAM-1/ICAM-2 double-deficient brain endothelium in vitro: the cytoplasmic tail of endothelial ICAM-1 is necessary for transendothelial migration of T cells

Lyck, R., Reiss, Y., Gerwin, N., et al. (2003) · Blood · doi:10.1182/blood-2003-02-0358 · Cited by 144 · see citing works

Culture-Induced Changes in Blood—Brain Barrier Transcriptome: Implications for Amino-Acid Transporters in vivo

Lyck, R., Ruderisch, N., Moll, A. G., Steiner, O., Cohen, C. D., Engelhardt, B., Makrides, V., & Verrey, F. (2009) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2009.72 · Cited by 140 · see citing works

Myosin-IIA and ICAM-1 Regulate the Interchange between Two Distinct Modes of T Cell Migration

Jacobelli, J., Bennett, F. C., Pandurangi, P., et al. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0803267 · Cited by 130 · see citing works

Intermediate‐affinity LFA‐1 binds α‐actinin‐1 to control migration at the leading edge of the T cell

Stanley, P., Smith, A. M., McDowall, A., et al. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601959 · Cited by 119 · see citing works

Human Brain Microvascular Endothelial Cells and Umbilical Vein Endothelial Cells Differentially Facilitate Leukocyte Recruitment and Utilize Chemokines for T Cell Migration

Man, S., Ubogu, E. E., Williams, K. A., Tucky, B. H., Callahan, M. K., & Ransohoff, R. M. (2008) · Clinical and Developmental Immunology · doi:10.1155/2008/384982 · Cited by 100 · see citing works

Human Blood-Brain Barrier Disruption by Retroviral-Infected Lymphocytes: Role of Myosin Light Chain Kinase in Endothelial Tight-Junction Disorganization

Afonso, P. V., Ozden, S., Prévost, M., Schmitt, C., Seilhean, D., Weksler, B. B., Couraud, P., Antoine Gessain, A., Romero, I. A., & Ceccaldi, P. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.4.2576 · Cited by 97 · see citing works

CCR7-mediated physiological lymphocyte homing involves activation of a tyrosine kinase pathway

Stein, J. V., Soriano, S. F., M’Rini, C., Nombela‐Arrieta, C., de Buitrago, G. G., Rodrı́guez-Frade, J. M., Mellado, M., Girard, J., & Martínez‐A, C. (2002) · Blood · doi:10.1182/blood-2002-03-0841 · Cited by 92 · see citing works

Shear flow–dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm

Schreiber, T. H., Shinder, V., Cain, D. W., et al. (2006) · Blood · doi:10.1182/blood-2006-07-032995 · Cited by 86 · see citing works

ICAM-1–mediated Endothelial Nitric Oxide Synthase Activation via Calcium and AMP-activated Protein Kinase Is Required for Transendothelial Lymphocyte Migration

Martinelli, R., Gegg, M. E., Longbottom, R. E., Adamson, P., Turowski, P., & Greenwood, J. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-06-0636 · Cited by 82 · see citing works

Pharmacological Targeting of ICAM-1 Signaling in Brain Endothelial Cells: Potential for Treating Neuroinflammation

Turowski, P., Adamson, P., & Greenwood, J. R. (2005) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-004-1380-0 · Cited by 81 · see citing works

Immunosurveillance modelled in vitro: naive and memory T cells spontaneously migrate across unstimulated microvascular endothelium

Rohnelt, R. (1997) · International Immunology · doi:10.1093/intimm/9.3.435 · Cited by 77 · see citing works

T cell interaction with ICAM-1-deficient endothelium in vitro: transendothelial migration of different T cell populations is mediated by endothelial ICAM-1 and ICAM-2

Reiss, Y., & Engelhardt, B. (1999) · International Immunology · doi:10.1093/intimm/11.9.1527 · Cited by 67 · see citing works

Peripheral blood CD4+ T lymphocytes from multiple sclerosis patients are characterized by higher PSGL-1 expression and transmigration capacity across a human blood-brain barrier-derived endothelial cell line

Bahbouhi, B., Berthelot, L., Pettré, S., Michel, L., Wiertlewski, S., Weksler, B. B., Romero, I. A., Miller, F., Couraud, P., Brouard, S., et al. (2009) · Journal of Leukocyte Biology · doi:10.1189/jlb.1008666 · Cited by 61 · see citing works

Evaluation of bEnd5 cell line as an in vitro model for the blood–brain barrier under normal and hypoxic/aglycemic conditions

Yang, T., Roder, K. E., & Abbruscato, T. J. (2007) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.21002 · Cited by 55 · see citing works

Differential expression of selectins by mouse brain capillary endothelial cells in vitro in response to distinct inflammatory stimuli

Coisne, C., Faveeuw, C., Delplace, Y., Dehouck, L., Miller, F., Cecchelli, R., & Dehouck, B. (2005) · Neuroscience Letters · doi:10.1016/j.neulet.2005.09.028 · Cited by 35 · see citing works

Impaired Induction of Adhesion Molecule Expression in Immortalized Endothelial Cells Leads to Functional Defects in Dynamic Interactions With Lymphocytes

Oostingh, G. J., Schlickum, S., Friedl, P., et al. (2007) · Journal of Investigative Dermatology · doi:10.1038/sj.jid.5700828 · Cited by 24 · see citing works

Regulation of fucosyltransferase-VII expression in peripheral lymph node high endothelial venules

(1998) · doi:10.1002/(sici)1521-4141(199810)28:10

Capture, crawl, cross: the T cell code to breach the blood–brain barriers

Engelhardt, B., & Ransohoff, R. M. (2012) · Trends in Immunology · doi:10.1016/j.it.2012.07.004 · Cited by 686 · see citing works

The anatomy and immunology of vasculature in the central nervous system

Mastorakos, P., & McGavern, D. (2019) · Science Immunology · doi:10.1126/sciimmunol.aav0492 · Cited by 333 · see citing works

Immune cell trafficking across the blood-brain barrier in the absence and presence of neuroinflammation

Marchetti, L., & Engelhardt, B. (2020) · Vascular Biology · doi:10.1530/vb-19-0033 · Cited by 304 · see citing works

Review: Leucocyte–endothelial cell crosstalk at the blood–brain barrier: A prerequisite for successful immune cell entry to the brain

Greenwood, J., Heasman, S. J., Alvarez, J. I., Prat, A., Lyck, R., & Engelhardt, B. (2011) · Neuropathology and Applied Neurobiology · doi:10.1111/j.1365-2990.2010.01140.x · Cited by 240 · see citing works

Human Brain Microvascular Endothelial Cells Derived from the BC1 iPS Cell Line Exhibit a Blood-Brain Barrier Phenotype

Katt, M. E., Xu, Z. S., Gerecht, S., & Searson, P. C. (2016) · PLOS ONE · doi:10.1371/journal.pone.0152105 · Cited by 129 · see citing works

ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier

Lyck, R., Lécuyer, M.-A., Abadier, M., Wyss, C. B., Matti, C., Rosito, M., Enzmann, G., Zeis, T., Michel, L., García Martín, A. B., et al. (2017) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x16678639 · Cited by 75 · see citing works

Co-Culture Models: Key Players in In Vitro Neurotoxicity, Neurodegeneration and BBB Modeling Studies

Monteiro, A. R., Barbosa, D. J., Remião, F., & Silva, R. (2024) · Biomedicines · doi:10.3390/biomedicines12030626 · Cited by 27 · see citing works

Viral interactions with the blood-brain barrier: old dog, new tricks

Hou, J., Baker, L. A., Zhou, L., & Klein, R. S. (2016) · Tissue Barriers · doi:10.1080/21688370.2016.1142492 · Cited by 24 · see citing works

Using Evans Blue Dye to Determine Blood‐Brain Barrier Integrity in Rodents

Goldim, M. P. D. S., Giustina, A. D., & Petronilho, F. C. (2019) · Current Protocols in Immunology · doi:10.1002/cpim.83 · Cited by 169 · see citing works

Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay

Waithe, O. Y., Peng, X., Childs, E. W., et al. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3429-5_14 · Cited by 16 · see citing works

MMP-Mediated Disruption of Claudin-5 in the Blood–Brain Barrier of Rat Brain After Cerebral Ischemia

Yang, Y. R., & Rosenberg, G. A. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-185-7_24 · Cited by 144 · see citing works

Factors affecting microvascular endothelial tight junction assembly and barrier function in the blood-brain barrier

Colgan, O. C. (2006) · Arrow@dit (Dublin Institute of Technology)