Augmentation and proliferation of T lymphocytes and Th-1 cytokines by Withania somnifera in stressed mice
Abstract
Stress has been associated with reports of both greater severity and prolongation of diseases in patients with the infectious origin as well as other immune-mediated diseases. Withania somnifera, an Indian medicinal plant used widely in the treatment of many clinical conditions in India, was investigated for its anti-stress properties using BALB/c mice subjected to chronic stress. The study aimed to investigate chronic stress-induced alterations on Th1 lymphocyte subset distribution and corresponding cytokine secretion patterns. Oral administration of chemically standardized and identified aqueous fraction of W. somnifera root (WS) at the graded doses of 25, 50, 100 and 200 mg/kg p.o. caused significant increase in the stress-induced depleted T-cell population and increased the expression of Th1 cytokines in chronically stressed mice.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Leaf extract in distilled water at 140 mg/kg/day alters the signaling pathway involving proapoptotic and tumor-promoting proteins to suppress the tumor growth of glioma cells.
“Alteration of the signaling”
From Withania somnifera (L.) Dunal (Ashwagandha): A comprehensive review on ethnopharmacology, pharmacotherapeutics, biomedicinal and toxicological aspects (2021) · cites this paper as [153]
Cites
Psychological Stress and Susceptibility to the Common Cold
Cohen, S., Tyrrell, D. A. J., & Smith, A. P. (1991) · New England Journal of Medicine · doi:10.1056/nejm199108293250903 · Cited by 1,712 · see citing works
Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review.
Mishra, L. C., Singh, B. B., & Dagenais, S. (2000) · PubMed · Cited by 776 · see citing works
Effects of stress on immune cell distribution. Dynamics and hormonal mechanisms.
Dhabhar, F. S., Miller, A. H., McEwen, B. S., & Spencer, R. L. (1995) · The Journal of Immunology · doi:10.4049/jimmunol.154.10.5511 · Cited by 730 · see citing works
Enhancing versus suppressive effects of stress hormones on skin immune function
Dhabhar, F. S., & McEwen, B. S. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.3.1059 · Cited by 672 · see citing works
Effects of stress on the immune system
Khansari, D. N., Murgo, A. J., & Faith, R. E. (1990) · Immunology Today · doi:10.1016/0167-5699(90)90069-l · Cited by 664 · see citing works
OECD guidelines for testing of chemicals
Wilhelm, K.-P., & Maibach, H. I. (2012) · CRC Press eBooks · doi:10.3109/9781841848570-66 · Cited by 629 · see citing works
Catecholamine-Induced Leukocytosis: Early Observations, Current Research, and Future Directions
Benschop, R. J., Rodriguez-Feuerhahn, M., & Schedlowski, M. (1996) · Brain Behavior and Immunity · doi:10.1006/brbi.1996.0009 · Cited by 521 · see citing works
Adams, R. (1982) · Immunology Today · doi:10.1016/0167-5699(82)90023-8 · Cited by 338 · see citing works
Moraska, A. F., Terrence Deak, B., Spencer, R. L., Roth, D., & Fleshner, M. (2000) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2000.279.4.r1321 · Cited by 325 · see citing works
Restraint stress in biomedical research: An update
Glavin, G. B., Paré, W. P., Sandbak, T., Bakke, H.-K., & Murison, R. (1994) · Neuroscience & Biobehavioral Reviews · doi:10.1016/0149-7634(94)90027-2 · Cited by 304 · see citing works
Social stress and the reactivation of latent herpes simplex virus type 1
Padgett, D. A., Sheridan, J. F., Dorne, J., Berntson, G. G., Candelora, J., & Glaser, R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.7231 · Cited by 266 · see citing works
Ayala, A., Herdon, C. D., Lehman, D. L., Ayala, C. A., & Ih, C. (1996) · Blood · doi:10.1182/blood.v87.10.4261.bloodjournal87104261 · Cited by 259 · see citing works
Stress, behavior, and immunity: Animal models and mediating mechanisms
Borysenko, M., & Borysenko, J. Z. (1982) · General Hospital Psychiatry · doi:10.1016/0163-8343(82)90028-7 · Cited by 160 · see citing works
Mastorakos, G. P., & Ilias, Ι. (2000) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2000.tb06220.x · Cited by 116 · see citing works
Wu, W., Yamaura, T., Murakami, K., Murata, J., Matsumoto, K., Watanabe, H., & Saiki, I. (2000) · Life Sciences · doi:10.1016/s0024-3205(00)00506-3 · Cited by 116 · see citing works
Stress, Affective Disorders, and Immune Function
Schindler, B. A. (1985) · Medical Clinics of North America · doi:10.1016/s0025-7125(16)31034-3 · Cited by 63 · see citing works
Valiathan, M. S. (2003) · Orient Longman eBooks · Cited by 53 · see citing works
Immunosuppressive properties of an ethyl acetate fraction from Euphorbia royleana
Bani, S., Kaul, A., Khan, B., Ahmad, S. F., Suri, K. A., Satti, N. K., Amina, M., & Qazi, G. N. (2005) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2004.12.017 · Cited by 51 · see citing works
Self-citationSingh, B., Chandan, B., Sharma, N., Singh, S., Khajuria, A., & Gupta, D. (2005) · Phytomedicine · doi:10.1016/j.phymed.2005.01.009 · Cited by 42 · see citing works
Adaptogenic activity of a fraction from the alcoholic extract of Gaertn.
Singh, B. (2001) · Phytomedicine · doi:10.1078/0944-7113-00038 · Cited by 21 · see citing works
Flamini, G., Cioni, P. L., Puleio, R., Morelli, I., & Panizzi, L. (1999) · Phytotherapy Research · doi:10.1002/(sici)1099-1573(199906)13:4 · Cited by 10 · see citing works
Clinical trial of pentazocine as analgesic in paediatric cases.
Ray, A. D., & Gupta, M. (1994) · PubMed · Cited by 4 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Medicinal Plants and Cancer Chemoprevention
Desai, A., Qazi, G., Ganju, R., El-Tamer, M., Singh, J., Saxena, A., Bedi, Y. S., Taneja, S., & Bhat, H. (2008) · Current Drug Metabolism · doi:10.2174/138920008785821657 · Cited by 613 · see citing works
Self-citationPharmacologic overview of Withania somnifera, the Indian Ginseng
Dar, N. J., Hamid, A., & Ahmad, M. (2015) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-2012-1 · Cited by 391 · see citing works
Paul, S., Chakraborty, S., Anand, U., Dey, S., Nandy, S., Ghorai, M., Saha, S. C., Patil, M. T., Kandimalla, R., Proćków, J., et al. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2021.112175 · Cited by 306 · see citing works
Dhama, K., Latheef, S. K., Saminathan, M., Samad, H. A., Karthik, K., Tiwari, R., Khan, R. U., Alagawany, M. M., Farag, M. R., Alam, G. M., et al. (2015) · International Journal of Pharmacology · doi:10.3923/ijp.2015.152.176 · Cited by 287 · see citing works
Speers, A. B., Cabey, K., Soumyanath, A., & Wright, K. M. (2021) · Current Neuropharmacology · doi:10.2174/1570159x19666210712151556 · Cited by 242 · see citing works
Çakici, N., van Beveren, N. J. M., Judge-Hundal, G., Koola, M. M., & Sommer, I. E. (2019) · Psychological Medicine · doi:10.1017/s0033291719001995 · Cited by 215 · see citing works
Mandlik, D. S., & Namdeo, A. G. (2020) · Journal of Dietary Supplements · doi:10.1080/19390211.2020.1741484 · Cited by 149 · see citing works
Natural Withanolides in the Treatment of Chronic Diseases
White, P. T., Subramanian, C., Motiwala, H. F., & Cohen, M. S. (2016) · Advances in Experimental Medicine and Biology · doi:10.1007/978-3-319-41334-1_14 · Cited by 139 · see citing works
Neurodegenerative diseases and Withania somnifera (L.): An update
Dar, N. J., & Ahmad, M. (2020) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2020.112769 · Cited by 123 · see citing works
Ancient medicine, modern use: Withania somnifera and its potential role in integrative oncology.
Winters, M. (2006) · PubMed · Cited by 115 · see citing works
Withania somnifera (L.) Dunal: Opportunity for Clinical Repurposing in COVID-19 Management
Saggam, A., Limgaokar, K., Borse, S., Chavan, P., Dixit, S., Tillu, G. S., & Patwardhan, B. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.623795 · Cited by 99 · see citing works
Tiwari, R., Chakrabort, S., Saminathan, M., Dhama, K., & Singh, S. V. (2014) · Journal of Biological Sciences · doi:10.3923/jbs.2014.77.94 · Cited by 93 · see citing works
Chauhan, P. S., Satti, N. K., Suri, K. A., Amina, M., & Bani, S. (2010) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2010.02.016 · Cited by 65 · see citing works
Self-citationModi, S. J., Tiwari, A., Ghule, C., Pawar, S., Saste, G., Jagtap, S., Singh, R., Deshmukh, A., Girme, A., & Hingorani, L. (2022) · Molecules · doi:10.3390/molecules27051476 · Cited by 68 · see citing works
The Role of Adaptogens in Prophylaxis and Treatment of Viral Respiratory Infections
Panossian, A. G., & Brendler, T. (2020) · Pharmaceuticals · doi:10.3390/ph13090236 · Cited by 63 · see citing works
Munien, P., Naidoo, Y., & Naidoo, G. (2015) · Planta · doi:10.1007/s00425-015-2341-1 · Cited by 61 · see citing works
Kour, K., Pandey, A., Suri, K. A., Satti, N. K., Gupta, K. K., & Bani, S. (2009) · International Immunopharmacology · doi:10.1016/j.intimp.2009.05.011 · Cited by 46 · see citing works
Self-citationIn Vivo Effects of Ashwagandha ( Withania somnifera ) Extract on the Activation of Lymphocytes
Mikolai, J., Erlandsen, A., Murison, A., Brown, K., Gregory, W. L., Raman-Caplan, P., & Zwickey, H. L. (2009) · The Journal of Alternative and Complementary Medicine · doi:10.1089/acm.2008.0215 · Cited by 43 · see citing works
Vaidya, V. G., Naik, N., Ganu, G. P., Parmar, V., Jagtap, S., Saste, G., Bhatt, A., Mulay, V. P., Girme, A., Modi, S. J., et al. (2023) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2023.117603 · Cited by 43 · see citing works
A unique immuno-stimulant steroidal sapogenin acid from the roots of Asparagus racemosus
Sharma, P., Chauhan, P. S., Dutt, P., Amina, M., Suri, K. A., Gupta, B. D., Suri, O. P., Dhar, K. L., Sharma, D., Gupta, V. K., et al. (2010) · Steroids · doi:10.1016/j.steroids.2010.12.006 · Cited by 36 · see citing works
Self-citationYoussouf, M. S., Kaiser, P. J., Singh, G. D., Singh, S. K., Bani, S., Gupta, V., Satti, N. K., Suri, K. A., & Johri, R. K. (2008) · International Immunopharmacology · doi:10.1016/j.intimp.2008.03.015 · Cited by 34 · see citing works
Self-citationCinnamomum zeylanicum extract inhibits proinflammatory cytokine TNF: in vitro and in vivo studies
Joshi, K., Awte, S., Bhatnagar, P., Walunj, S. S., Gupta, R., Joshi, S., Sabharwal, S. G., Bani, S., & Padalkar, A. S. (2010) · Research in Pharmaceutical Biotechnology · doi:10.5897/rpb.9000004 · Cited by 32 · see citing works
Self-citationLatheef, S. K., Dhama, K., Samad, H. A., Wani, M. Y., Kumar, M. A., Palanivelu, M., Malik, Y. S., Singh, S. D., & Singh, R. P. (2017) · VirusDisease · doi:10.1007/s13337-016-0355-3 · Cited by 31 · see citing works
Lee, S. H., Lillehoj, H. S., Chun, H., Tuo, W., Park, H., Cho, S., Lee, Y., & Lillehoj, E. P. (2007) · Nutrition Research · doi:10.1016/j.nutres.2007.04.001 · Cited by 26 · see citing works
Phytochemical Genomics of Ashwagandha
Sangwan, N. S., Tripathi, S., Srivastava, Y., Mishra, B., & Pandey, N. (2017) · Springer eBooks · doi:10.1007/978-3-319-59192-6_1 · Cited by 25 · see citing works
Biodiversity, Biology and Conservation of Medicinal Plants of the Thar Desert
Arora, J., Goyal, S., & Ramawat, K. G. (2009) · Springer eBooks · doi:10.1007/978-3-642-02550-1_1 · Cited by 25 · see citing works
Ahirwal, L., Singh, S., Dubey, M. K., Bharti, V., Mehta, A., & Shukla, S. (2015) · Archives of Biological Sciences · doi:10.2298/abs141027018a · Cited by 23 · see citing works
Hanieh, H., Narabara, K., Tanaka, Y., Gu, Z., Asaki, A. B. E., & Kondo, Y. (2011) · Animal Science Journal · doi:10.1111/j.1740-0929.2011.00918.x · Cited by 18 · see citing works
Mofed, D., Ahmed, W., Zekri, A. N., Said, O., Rahouma, M., & Faraag, A. H. I. (2020) · Advances in Microbiology · doi:10.4236/aim.2020.109035 · Cited by 17 · see citing works
TNF-α inhibitory effect ofEuphorbia hirtain rats
Ahmad, S. F., Bani, S., Sultan, P., Ali, S. A., Bakheet, S. A., Attia, S. M., & Abd‐Allah, A. R. A. (2013) · Pharmaceutical Biology · doi:10.3109/13880209.2012.734315 · Cited by 17 · see citing works
Self-citationEl‐Boshy, M., Abdalla, O. M., Risha, A., & Moustafa, F. A. (2013) · ISRN Veterinary Science · doi:10.1155/2013/153427 · Cited by 13 · see citing works
Bharani, K. K., Devarasetti, A. K., Carey, L., Khurana, A., Kollipaka, R., Hanuman, D. D. V., Chetla, V. S., & Banothu, A. K. (2024) · Veterinary Medicine and Science · doi:10.1002/vms3.1556 · Cited by 10 · see citing works
Kiriacos, C. J., Khedr, M. R., Tadros, M., & Youness, R. A. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.837408 · Cited by 10 · see citing works
Ashwagandha (Withania somnifera L.) - the plant with proven health-promoting properties
Połumackanycz, M., Forencewicz, A., Wesołowski, M., & Viapiana, A. (2020) · Farmacja Polska · doi:10.32383/farmpol/127824 · Cited by 9 · see citing works
Trivedi, M. K., Gangwar, M., Mondal, S. C., & Jana, S. (2017) · Oriental Pharmacy and Experimental Medicine · doi:10.1007/s13596-016-0255-5 · Cited by 7 · see citing works
Kubukgolla, W. W., Attanayake, A. M. H. S., & Jayaweera, J. A. A. S. (2021) · International Journal of Ayurvedic Medicine · doi:10.47552/ijam.v12i4.2220 · Cited by 5 · see citing works
Withanolides: A Prospective Drug for Infectious and Tropical Diseases
Maurya, R. (2017) · Springer eBooks · doi:10.1007/978-3-319-59192-6_5 · Cited by 5 · see citing works
Nobarı, H., Parnian-Khajehdizaj, N., Johnson, K. E., & Ramos-Álvarez, J. J. (2026) · Nutrition & Metabolism · doi:10.1186/s12986-026-01093-5 · Cited by 5 · see citing works
Charmas, M., Jówko, E., Długołęcka, B., Klusiewicz, A., Przybylska, I., & Galczak-Kondraciuk, A. (2025) · Nutrients · doi:10.3390/nu17203245 · Cited by 4 · see citing works
Zoheir, K. M. A., Abdrabou, A., Darwish, A. M., Abdelhafez, M. A., & Mahrous, K. F. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.998247 · Cited by 4 · see citing works
Mahadevan, M. V., Kumarpillai, G., Gupta, R., Morde, A. A., Patni, P., Srinivas, S. S., Bhuvanendran, A., & Phanindra, A. (2025) · Advances in Therapy · doi:10.1007/s12325-025-03327-z · Cited by 3 · see citing works
Suresh Ramnath, N. A. I. K., Gavankar, C., & Thakare, V. N. (2015) · Pharmacologia · doi:10.5567/pharmacologia.2015.168.177 · Cited by 3 · see citing works
Yadav, A. K., Ojha, M. D., & Hariprasad, P. (2022) · Computers in Biology and Medicine · doi:10.1016/j.compbiomed.2022.105598 · Cited by 3 · see citing works
Sehrawat, A., Samanta, S. K., Kim, S., Hahm, E., & Singh, S. V. (2017) · Springer eBooks · doi:10.1007/978-3-319-59192-6_8 · Cited by 3 · see citing works
Hussain, S. M. (2013) · Al-Mustansiriyah Journal of Sciences · Cited by 2 · see citing works
Ethnopharmacology Approaches for Botanical Immunomodulators and Chemoprotectants in Cancer Therapy
Patwardhan, B., & Gautam, M. (2007) · Annals of traditional chinese medicine · doi:10.1142/9789812709301_0011 · Cited by 2 · see citing works
Some Representative Medicinal Halophytes in Asia
Öztürk, M., Altay, V., Nazish, M., Ahmad, M., & Zafar, M. (2023) · Springer eBooks · doi:10.1007/978-3-031-21944-3_2 · Cited by 1 · see citing works
Chengappa, K. N. R., Gannon, J. M., Acharya, L., & Rai, A. (2017) · Springer eBooks · doi:10.1007/978-3-319-59192-6_17 · Cited by 1 · see citing works
A REVIEW ON WITHANIA SOMNIFERA (L.) DUNAL- AS AN IMPORTANT AYURVEDA PLANT
Pandya, D., Mankad, A., & Pandya, H. (2022) · International Association of Biologicals and Computational Digest · doi:10.56588/iabcd.v1i1.28 · Cited by 2 · see citing works
Withania somnifera (L.) Dunal (Solanaceae)
Akbar, S. (2020) · Springer eBooks · doi:10.1007/978-3-030-16807-0_197 · Cited by 2 · see citing works
(2013) · Elsevier eBooks · doi:10.1016/b978-0-443-06992-5.00010-4 · Cited by 1 · see citing works
The morphology and chemical composition of the trichomes of Withania somnifera (Solanaceae)
Munien, P. (2014) · ResearchSpace (University of KwaZulu-Natal)
Examples of Useful Nutritional Supplement Formulations
(2007) · Nutrition in exercise and sport · doi:10.1201/9781420043815.ch13
Kumar, S. S., Sharma, B., & Suhail, M. (2013) · International Journal of Pharma and Bio Sciences
Anti-inflammatory Agents for Patients with Schizophrenia
van Beveren, N. J. M., Çakici, N., & Sommer, I. E. (2021) · Springer eBooks · doi:10.1007/978-3-030-71229-7_22
Como os adaptógenos influenciam a performance cognitivo-mental: uma revisão integrativa
Zago, Â., & Negro‐Dellacqua, M. (2022) · Research Society and Development · doi:10.33448/rsd-v11i10.32635
Adoption of biotechnologies for Withania somnifera (L.) Dunal for sustainable utilization
Shah, S., Vashisth, D., Shasany, A. K., Ambati, R. R., & Ravishankar, G. A. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-21818-7.00013-7
Marder, S. R., Kelly, D. L., DeVera, G., Zaranski, J., Whelan, F., Sugar, C. A., & Buchanan, R. W. (2026) · Journal of Clinical Psychopharmacology · doi:10.1097/jcp.0000000000002229
Allemailem, K. S. (2026) · Journal of King Saud University - Science · doi:10.25259/jksus_1523_2025
- Authors
- Khan, B., Ahmad, S. F., Bani, S., Kaul, A., Suri, K. A., Satti, N. K., Athar, M., & Qazi, G. N.
- Affiliations
- National Institute for Interdisciplinary Science and Technology (IN)
- Indian Institute of Integrative Medicine (IN)
- Jamia Hamdard (IN)
- Corresponding author
- Bani, Sarang
- Venue
- International Immunopharmacology
- Publisher
- Elsevier BV
- Published
- 2006-09
- Volume
- 6
- Issue
- 9
- Pages
- 1394-1403
- ISSN
- 1567-5769 (ISSN-L)
- OpenAlex
- W2064718322
- Cited by
- 61
- References
- 22
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 6 · 2021: 5 · 2020: 6 · 2019: 1 · 2017: 6 · 2016: 1 · 2015: 5 · 2014: 3 · 2013: 6
- FWCI
- 7.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Immunopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Medicinal Plants and Bioactive Compounds
- Health Sciences › Medicine › Physiology › Biofield Effects and Biophysics
- Keywords
- Withania somnifera, chronic stress, Th1 cells, Th1 cytokines, T cell proliferation, immunomodulation, anti-stress activity, aqueous root extract, stress-induced immunosuppression, BALB/c mice
- MeSH terms
- Adjuvants, Immunologic, Administration, Oral, Animals, Male, Mice, Inbred BALB C, Plant Extracts, Stress, Physiological, Cytokines, Th1 Cells, Plant Roots, Withania, Cell Proliferation, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
