Cited by 61Sulfur Compounds in BiologyInflammatory Bowel DiseaseGastrointestinal motility and disorders

Hydrogen sulfide improves colonic barrier integrity in DSS-induced inflammation in Caco-2 cells and mice

Zhao, Hongyu, Yan, Rui, Zhou, Xiaogang, Ji, Fang, Zhang, Bing·2016·International Immunopharmacology

Abstract

Intestinal barrier involves in the pathogeny of inflammatory bowel disease (IBD) and hydrogen sulfide (H2S) has been reported to improve intestinal barrier integrity. Thus, this study investigated the effects of GYY4137, a slow-release H2S donor, on DSS-induced inflammation and intestinal dysfunction. In vitro model, cellular permeability was significantly increased and expression of tight junctions (ZO-1, Cauldin4, and Occludin) was downregulated in Caco-2 cells. GYY4137 treatment markedly attenuated DSS-induced inflammation and barrier dysfunction. Cystathionine β-synthase (CBS)-siRNA transfection further demonstrated that endogenous H2S system involves in DSS-induced inflammation and mediates barrier function. In vivo model, DSS exposure caused colonic inflammation and injury in mice and GYY4137 injection alleviated inflammatory response and improved intestinal barrier via reducing intestinal permeability and upregulating of tight junctions. In conclusion, endogenous H2S system involves in DSS-induced inflammation and H2S addition alleviated inflammation and intestinal dysfunction in vitro and in vivo.

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Crossref · 10.1016/j.intimp.2016.07.020

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Authors
Zhao, H., Yan, R., Zhou, X., Ji, F., & Zhang, B.
Affiliations
Xinjiang Medical University (CN)
Corresponding author
Zhang, Bing
Venue
International Immunopharmacology
Publisher
Elsevier BV
Published
2016-10
Volume
39
Pages
121-127
ISSN
1567-5769 (ISSN-L)
OpenAlex
W2499516840
Cited by
61
References
33
Citations by year
2026: 6 · 2025: 10 · 2024: 8 · 2023: 9 · 2022: 7 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 2 · 2017: 5 · 2016: 1
FWCI
2.87
Type
journal article
Language
en
Open access
closed
Locations
  • International Immunopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
hydrogen sulfide, GYY4137, intestinal barrier integrity, DSS-induced colitis, inflammatory bowel disease, tight junctions, intestinal permeability, cystathionine beta-synthase, Caco-2 cells, colonic inflammation
MeSH terms
Animals, Cell Membrane Permeability, Colitis, Colon, Cystathionine beta-Synthase, Female, Humans, Hydrogen Sulfide, Mice, Inbred Strains, Morpholines, Organothiophosphorus Compounds, Dextran Sulfate, Caco-2 Cells, Tight Junctions, RNA, Small Interfering, Mice

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