Endogenous hydrogen sulfide is an anti-inflammatory molecule in dextran sodium sulfate-induced colitis in mice
Abstract
Background Endogenous hydrogen sulfide (H(2)S) is increasingly being recognized as an important gaseous physiological mediator. Accumulating evidence shows the functions of H(2)S in various models of disease, but rarely in colitis. In this study, we investigated the role of endogenous H(2)S in a dextran sodium sulfate (DSS)-induced colitis model.Methods Acute colitis was induced using 8% DSS in male BALB/c mice. The mRNA expression of cystathionine γ-lyase (CSE), the primary synthetase of H(2)S in the gastrointestinal tract, and cystathionine-β-synthetase (CBS) was measured by real-time RT-PCR. The amount of H(2)S in the colonic mucosa was measured by gas chromatography. Colitis severity was evaluated clinically, histologically, and biochemically under the condition of co-treatment with DL-propargylglycine (PAG), an irreversible CSE inhibitor, and sodium sulfide (Na(2)S), an H(2)S donor.Results The mRNA expression levels of CSE and CBS, and the H(2)S content in the colonic mucosa were increased with time after DSS administration. The disease activity index, which was determined by weight loss, stool consistency, and intestinal bleeding, increased after DSS administration. PAG significantly enhanced the increase in the disease activity index scores. PAG also significantly increased tissue-associated myeloperoxidase activity and thiobarbituric acid-reactive substances in the inflamed mucosa. Moreover, Na(2)S counteracted these effects of PAG.Conclusions Taken together, the results indicated that the inhibition of endogenous H(2)S generation caused the deterioration of DSS-induced colitis. We conclude that physiological H(2)S might act as an anti-inflammatory molecule in colitis.
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Lower concentrations of endogenously derived H2S exert beneficial cytoprotective, antioxidant and anti-inflammatory actions.
“On one hand, it appears that lower concentrations of endogenously derived H2S exert beneficial cytoprotective, antioxidant and anti-inflammatory actions[344,348–351]”
From Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health (2017) · cites this paper as [351]
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- Authors
- Hirata, I., Naito, Y., Takagi, T., Mizushima, K., Suzuki, T., Omatsu, T., Handa, O., Ichikawa, H., Ueda, H., & Yoshikawa, T.
- Affiliations
- Kyoto Prefectural University of Medicine (JP)
- Doshisha University (JP)
- Corresponding author
- Naito, Yuji
- Venue
- Digestive Diseases and Sciences
- Published
- 2010-10-28
- Volume
- 56
- Issue
- 5
- Pages
- 1379-1386
- ISSN
- 0163-2116 (ISSN-L)
- 1573-2568
- OpenAlex
- W1999962647
- Cited by
- 72
- References
- 35
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 8 · 2022: 7 · 2021: 2 · 2020: 6 · 2019: 7 · 2018: 2 · 2017: 5 · 2016: 6 · 2015: 3 · 2014: 5 · 2013: 6 · 2012: 5
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Digestive Diseases and Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (22590706)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Health Sciences › Medicine › Surgery › Hydrogen's biological and therapeutic effects
- Keywords
- hydrogen sulfide, DSS-induced colitis, cystathionine gamma-lyase, cystathionine beta-synthase, anti-inflammatory activity, DL-propargylglycine, sodium sulfide, myeloperoxidase, oxidative stress, colonic mucosa, inflammatory bowel disease
- MeSH terms
- Alkynes, Animals, Colitis, Cystathionine beta-Synthase, Cystathionine gamma-Lyase, Glycine, Hydrogen Sulfide, Male, Mice, Inbred BALB C, RNA, Messenger, Sulfates, Time Factors, Gene Expression Regulation, Enzymologic, Dextran Sulfate, Mice
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