The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes
Open accessAbstract
Background Patients with severe asthma have increased granulocytes in their sputum compared to patients with mild to moderate asthma. Objective We hypothesized that inflammatory granulocytes in sputum may identify specific asthma severity phenotypes and are associated with different patterns of inflammatory proteins in sputum supernatants. Methods This hypothesis was tested in 242 asthmatics enrolled in the Severe Asthma Research Program who provided sputum samples for cell count, differential cell determinations, cell lysates for Western blot, and supernatant analyses by inflammatory protein microarrays and ELISAs. ANOVA and multiple linear regression models tested mediator associations. Results Stratified by sputum granulocytes, < or ≥2%eosinophils and < or ≥40%neutrophils, subjects with both increased eosinophils and neutrophils had the lowest lung function, increased symptoms and healthcare utilization. Subjects with elevated eosinophils with or without increased neutrophils had significantly increased FeNO, serum eosinophils and greater frequency of daily β-agonist use. Microarray data, stratified by granulocytes revealed 25–28 inflammatory proteins increased >2-fold in sputa with ≥40% neutrophils. Microarray analyses stratified by severity of asthma, identified 6–9 proteins increased >2-fold in sputa in subjects with severe asthma compared to nonsevere asthma. ELISA data, stratified by sputum granulocytes, showed significant increases in BDNF, IL-1β, and MIP-3α/CCL20 for those with ≥40%neutrophils; these mediators demonstrated positive associations with neutrophil counts. Conclusion Combined increased sputum eosinophils and neutrophils identified asthmatics with the lowest lung function and worse asthma control, increased symptoms and healthcare requirements. Inflammatory protein analyses of sputum supernatants found novel mediators increased in asthmatics, predominantly associated with increased sputum neutrophils.
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Cluster analysis and clinical asthma phenotypes
Haldar, P., Pavord, I. D., Shaw, D. E., Berry, M. A., Thomas, M., Brightling, C. E., Wardlaw, A. J., & Green, R. H. (2008) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200711-1754oc · Cited by 1,987
Airway Epithelial Cells Release MIP-3α/CCL20 in Response to Cytokines and Ambient Particulate Matter
Reibman, J., Hsu, Y., Chen, L. C., Bleck, B., & Gordon, T. (2003) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2002-0095oc · Cited by 262
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International ERS/ATS guidelines on definition, evaluation and treatment of severe asthma
Chung, K. F., Wenzel, S. E., Brozek, J. L., Bush, A., Castro, M., Sterk, P. J., Adcock, I. M., Bateman, E. D., Bel, E. H., Bleecker, E. R., et al. (2014) · European Respiratory Journal · doi:10.1183/09031936.00202013 · Cited by 3,926
Self-citationSputum neutrophil counts are associated with more severe asthma phenotypes using cluster analysis
Moore, W. C., Hastie, A. T., Li, X., Li, H., Busse, W. W., Jarjour, N. N., Wenzel, S. E., Peters, S. P., Meyers, D. A., & Bleecker, E. R. (2014) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2013.10.011 · Cited by 600
Self-citationRole for NLRP3 Inflammasome–mediated, IL-1β–Dependent Responses in Severe, Steroid-Resistant Asthma
Kim, R. Y., Pinkerton, J. W., Essilfie, A. T., Robertson, A. A. B., Baines, K. J., Brown, A. C., Mayall, J. R., Ali, M. K., Starkey, M. R., Hansbro, N. G., et al. (2017) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201609-1830oc · Cited by 435
Szefler, S. J., Wenzel, S., Brown, R., Erzurum, S. C., Fahy, J. V., Hamilton, R. G., Hunt, J. F., Kita, H., Liu, A. H., Panettieri, R. A., et al. (2012) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2011.12.979 · Cited by 395
IL-17 in Severe Asthma. Where Do We Stand?
Chesné, J., Braza, F., Mahay, G., Brouard, S., Aronica, M., & Magnan, A. (2014) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201405-0859pp · Cited by 377
Metadata
- Authors
- Hastie, A. T., Moore, W. C., Meyers, D. A., Vestal, P. L., Li, H., Peters, S. P., & Bleecker, E. R.
- Venue
- Journal of Allergy and Clinical Immunology
- Publisher
- Elsevier BV
- Published
- 2010-05
- Volume
- 125
- Issue
- 5
- Pages
- 1028-1036.e13
- ISSN
- 0091-6749 (ISSN-L)
- OpenAlex
- W2051954214
- Cited by
- 463
- References
- 2
- FWCI
- 20.35
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Hastie, A. T., Moore, W. C., Meyers, D. A., Vestal, P. L., Li, H., Peters, S. P., & Bleecker, E. R. (2010). Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes. Journal of Allergy and Clinical Immunology. https://doi.org/10.1016/j.jaci.2010.02.008
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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