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Cited by 35Probiotics and Fermented FoodsPolysaccharides Composition and ApplicationsAdvanced Scientific Research Methods

Growth and Exopolysaccharide Yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in Batch and Continuous Bioreactor Experiments at Constant PH

Mende, Susann, Krzyzanowski, Leona, Weber, Jost, Jaros, Doris, Rohm, Harald·2012·Journal of Bioscience and Bioengineering
DOI 10.1016/j.jbiosc.2011.10.012
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Abstract

Some Lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (EPS) and are therefore highly important for the dairy industry as starter cultures. The aim of this study was to investigate the nutritional requirements for growth and EPS production of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081. A medium was developed from a semi-defined medium (SDM) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, salts, sodium formate and orotic acid) were added. Constant pH batch fermentation with the modified medium resulted in an EPS yield of approximately 210 mg glucose equivalents per liter medium. This was a 10-fold increase over flask cultivation of this strain in SDM. Although not affecting cell growth, the mixture of salts enhanced the EPS synthesis. Whereas EPS production was approximately 12 mg/g dry biomass without salt supplementation, a significantly higher yield (approximately 20 mg/g dry biomass) was observed after adding the salt mixture. In continuous fermentation, a maximal EPS concentration was obtained at a dilution rate of 0.31/h (80 mg EPS/L), which corresponded to a specific EPS production of 49 mg/g dry biomass.

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  • For Lb. delbrüeckii subsp. bulgaricus DSM 20081, the EPS yield was higher when incubated between 30 °C to 40 °C, whereas EPS production was decreased by almost half when incubated at 45 °C.

    “For the Lb. delbrüeckii subsp. bulgaricus DSM 20081 strain, the EPS yield was higher when incubated between 30 °C to 40 °C, whereas the EPS production was decreased by almost half when incubated at 45 °C [67].”

    From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [67]

  • Sub-optimal temperature is the optimal temperature for higher EPS production.

    “In turn, several studies reported that sub-optimal temperature is the optimal temperature for higher EPS production [59,67]”

    From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [67]

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Authors
Mende, S., Krzyzanowski, L., Weber, J., Jaros, D., & Rohm, H.
Affiliations
Technische Universität Dresden (DE)
Corresponding author
Jaros, Doris
ORCID
Venue
Journal of Bioscience and Bioengineering
Publisher
Elsevier BV
Published
2012-02
Volume
113
Issue
2
Pages
185-191
ISSN
  • 1347-4421 (ISSN-L)
  • 1389-1723
DOI
10.1016/j.jbiosc.2011.10.012
OpenAlex
W2036159388
Cited by
35
References
55
Citations by year
2025: 3 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 2 · 2013: 3 · 2012: 1
FWCI
2.81
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Bioscience and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
Keywords
Lactobacillus bulgaricus, exopolysaccharide production, starter cultures, salt supplementation, continuous fermentation, dilution rate, cell growth
MeSH terms
Culture Media, Fermentation, Glucose, Hydrogen-Ion Concentration, Lactose, Polysaccharides, Bacterial, Biomass, Bioreactors, Lactobacillus delbrueckii, Batch Cell Culture Techniques

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